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Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus

OBJECTIVE: This study was conducted to identify the biomarkers and mechanisms associated with systemic lupus erythematosus(SLE) at a transcriptome level. METHODS: Microarray datasets were downloaded, and differentially expressed genes (DEGs) were identified. Enrichment and protein–protein interactio...

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Autores principales: Xiao, Lu, Zhan, Feng, Lin, Shudian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219551/
https://www.ncbi.nlm.nih.gov/pubmed/35757684
http://dx.doi.org/10.3389/fimmu.2022.844025
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author Xiao, Lu
Zhan, Feng
Lin, Shudian
author_facet Xiao, Lu
Zhan, Feng
Lin, Shudian
author_sort Xiao, Lu
collection PubMed
description OBJECTIVE: This study was conducted to identify the biomarkers and mechanisms associated with systemic lupus erythematosus(SLE) at a transcriptome level. METHODS: Microarray datasets were downloaded, and differentially expressed genes (DEGs) were identified. Enrichment and protein–protein interaction networks were analyzed, and hub genes were discovered. The levels of top 10 hub genes were validated by another dataset. The diagnostic accuracy of the hub genes was evaluated with the area under the curve of the receiver operating characteristic curve (ROC-AUC). The odds ratios (OR) and 95% confidence intervals (CI) of the relationship between clinical manifestations and hub genes were estimated with multivariable logistic regression. The relationships between the expression levels of the 10 identified hub genes and SLEDAI scores were subjected to linear correlation analysis. Changes in the expression levels of the hub genes during patient follow-up were examined through one-way repeated measures ANOVA. RESULTS: A total of 136 DEGs were identified. Enrichment analysis indicated that DEGs were primarily enriched in type I interferon-associated pathways. The identified hub genes were verified by the GSE65391 dataset. The 10 hub genes had good diagnostic performances. Seven (except IFI6, OAS1 and IFIT3) of the 10 hub genes were positively associated with SLEDAI. The combination models of IFIT3, ISG15, MX2, and IFIH1 were effective in diagnosing mucosal ulcers among patients with SLE. The expression levels of IRF7, IFI35, IFIT3, and ISG15 decreased compared with the baseline expression (not significantly). CONCLUSIONS: In this work, the clinical values of the identified hub genes in SLE were demonstrated.
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spelling pubmed-92195512022-06-24 Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus Xiao, Lu Zhan, Feng Lin, Shudian Front Immunol Immunology OBJECTIVE: This study was conducted to identify the biomarkers and mechanisms associated with systemic lupus erythematosus(SLE) at a transcriptome level. METHODS: Microarray datasets were downloaded, and differentially expressed genes (DEGs) were identified. Enrichment and protein–protein interaction networks were analyzed, and hub genes were discovered. The levels of top 10 hub genes were validated by another dataset. The diagnostic accuracy of the hub genes was evaluated with the area under the curve of the receiver operating characteristic curve (ROC-AUC). The odds ratios (OR) and 95% confidence intervals (CI) of the relationship between clinical manifestations and hub genes were estimated with multivariable logistic regression. The relationships between the expression levels of the 10 identified hub genes and SLEDAI scores were subjected to linear correlation analysis. Changes in the expression levels of the hub genes during patient follow-up were examined through one-way repeated measures ANOVA. RESULTS: A total of 136 DEGs were identified. Enrichment analysis indicated that DEGs were primarily enriched in type I interferon-associated pathways. The identified hub genes were verified by the GSE65391 dataset. The 10 hub genes had good diagnostic performances. Seven (except IFI6, OAS1 and IFIT3) of the 10 hub genes were positively associated with SLEDAI. The combination models of IFIT3, ISG15, MX2, and IFIH1 were effective in diagnosing mucosal ulcers among patients with SLE. The expression levels of IRF7, IFI35, IFIT3, and ISG15 decreased compared with the baseline expression (not significantly). CONCLUSIONS: In this work, the clinical values of the identified hub genes in SLE were demonstrated. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9219551/ /pubmed/35757684 http://dx.doi.org/10.3389/fimmu.2022.844025 Text en Copyright © 2022 Xiao, Zhan and Lin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Xiao, Lu
Zhan, Feng
Lin, Shudian
Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus
title Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus
title_full Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus
title_fullStr Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus
title_full_unstemmed Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus
title_short Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus
title_sort clinical values of the identified hub genes in systemic lupus erythematosus
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219551/
https://www.ncbi.nlm.nih.gov/pubmed/35757684
http://dx.doi.org/10.3389/fimmu.2022.844025
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