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Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates

The World Health Organization has identified antimicrobial resistance as a public health emergency and developed a global priority pathogens list of antibiotic-resistant bacteria that can be summarized in the acronym ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acineto...

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Autores principales: Bivona, Dalida Angela, Mirabile, Alessia, Bonomo, Carmelo, Bonacci, Paolo Giuseppe, Stracquadanio, Stefano, Marino, Andrea, Campanile, Floriana, Bonaccorso, Carmela, Fortuna, Cosimo Gianluca, Stefani, Stefania, Musso, Nicolò, Bongiorno, Dafne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219758/
https://www.ncbi.nlm.nih.gov/pubmed/35740173
http://dx.doi.org/10.3390/antibiotics11060767
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author Bivona, Dalida Angela
Mirabile, Alessia
Bonomo, Carmelo
Bonacci, Paolo Giuseppe
Stracquadanio, Stefano
Marino, Andrea
Campanile, Floriana
Bonaccorso, Carmela
Fortuna, Cosimo Gianluca
Stefani, Stefania
Musso, Nicolò
Bongiorno, Dafne
author_facet Bivona, Dalida Angela
Mirabile, Alessia
Bonomo, Carmelo
Bonacci, Paolo Giuseppe
Stracquadanio, Stefano
Marino, Andrea
Campanile, Floriana
Bonaccorso, Carmela
Fortuna, Cosimo Gianluca
Stefani, Stefania
Musso, Nicolò
Bongiorno, Dafne
author_sort Bivona, Dalida Angela
collection PubMed
description The World Health Organization has identified antimicrobial resistance as a public health emergency and developed a global priority pathogens list of antibiotic-resistant bacteria that can be summarized in the acronym ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacterales species), reminding us of their ability to escape the effect of antibacterial drugs. We previously tested new heteroaryl-ethylene compounds in order to define their spectrum of activity and antibacterial capability. Now, we focus our attention on PB4, a compound with promising MIC and MBC values in all conditions tested. In the present study, we evaluate the activity of PB4 on selected samples of ESKAPE isolates from nosocomial infections: 14 S. aureus, 6 E. faecalis, 7 E. faecium, 12 E. coli and 14 A. baumannii. Furthermore, an ATCC control strain was selected for all species tested. The MIC tests were performed according to the standard method. The PB4 MIC values were within very low ranges regardless of bacterial species and resistance profiles: from 0.12 to 2 mg/L for S. aureus, E. faecalis, E. faecium and A. baumannii. For E. coli, the MIC values obtained were slightly higher (4–64 mg/L) but still promising. The PB4 heteroaryl-ethylenic compound was able to counteract the bacterial growth of both high-priority Gram-positive and Gram-negative clinical strains. Our study contributes to the search for new molecules that can fight bacterial infections, in particular those caused by MDR bacteria in hospitals. In the future, it would be interesting to evaluate the activity of PB4 in animal models to test for its toxicity.
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spelling pubmed-92197582022-06-24 Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates Bivona, Dalida Angela Mirabile, Alessia Bonomo, Carmelo Bonacci, Paolo Giuseppe Stracquadanio, Stefano Marino, Andrea Campanile, Floriana Bonaccorso, Carmela Fortuna, Cosimo Gianluca Stefani, Stefania Musso, Nicolò Bongiorno, Dafne Antibiotics (Basel) Article The World Health Organization has identified antimicrobial resistance as a public health emergency and developed a global priority pathogens list of antibiotic-resistant bacteria that can be summarized in the acronym ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacterales species), reminding us of their ability to escape the effect of antibacterial drugs. We previously tested new heteroaryl-ethylene compounds in order to define their spectrum of activity and antibacterial capability. Now, we focus our attention on PB4, a compound with promising MIC and MBC values in all conditions tested. In the present study, we evaluate the activity of PB4 on selected samples of ESKAPE isolates from nosocomial infections: 14 S. aureus, 6 E. faecalis, 7 E. faecium, 12 E. coli and 14 A. baumannii. Furthermore, an ATCC control strain was selected for all species tested. The MIC tests were performed according to the standard method. The PB4 MIC values were within very low ranges regardless of bacterial species and resistance profiles: from 0.12 to 2 mg/L for S. aureus, E. faecalis, E. faecium and A. baumannii. For E. coli, the MIC values obtained were slightly higher (4–64 mg/L) but still promising. The PB4 heteroaryl-ethylenic compound was able to counteract the bacterial growth of both high-priority Gram-positive and Gram-negative clinical strains. Our study contributes to the search for new molecules that can fight bacterial infections, in particular those caused by MDR bacteria in hospitals. In the future, it would be interesting to evaluate the activity of PB4 in animal models to test for its toxicity. MDPI 2022-06-03 /pmc/articles/PMC9219758/ /pubmed/35740173 http://dx.doi.org/10.3390/antibiotics11060767 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bivona, Dalida Angela
Mirabile, Alessia
Bonomo, Carmelo
Bonacci, Paolo Giuseppe
Stracquadanio, Stefano
Marino, Andrea
Campanile, Floriana
Bonaccorso, Carmela
Fortuna, Cosimo Gianluca
Stefani, Stefania
Musso, Nicolò
Bongiorno, Dafne
Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates
title Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates
title_full Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates
title_fullStr Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates
title_full_unstemmed Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates
title_short Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates
title_sort heteroaryl-ethylenes as new effective agents for high priority gram-positive and gram-negative bacterial clinical isolates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219758/
https://www.ncbi.nlm.nih.gov/pubmed/35740173
http://dx.doi.org/10.3390/antibiotics11060767
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