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Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility

Infertility is one of the most rapidly increasing global health concerns of the 21st century. Embryo quality and endometrial thickness and receptivity are the main factors for successful embryo implantation and pregnancy development. Nevertheless, until now, there has been a lack of understanding ab...

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Autores principales: Skliutė, Giedrė, Baušytė, Raminta, Ramašauskaitė, Diana, Navakauskienė, Rūta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219839/
https://www.ncbi.nlm.nih.gov/pubmed/35740346
http://dx.doi.org/10.3390/biomedicines10061324
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author Skliutė, Giedrė
Baušytė, Raminta
Ramašauskaitė, Diana
Navakauskienė, Rūta
author_facet Skliutė, Giedrė
Baušytė, Raminta
Ramašauskaitė, Diana
Navakauskienė, Rūta
author_sort Skliutė, Giedrė
collection PubMed
description Infertility is one of the most rapidly increasing global health concerns of the 21st century. Embryo quality and endometrial thickness and receptivity are the main factors for successful embryo implantation and pregnancy development. Nevertheless, until now, there has been a lack of understanding about the regulation of human endometrium function and its structure. This raises the demand for more research of the human endometrium in these fields. In our study, we analyzed the genetic and epigenetic changes of endometrial tissue’s samples isolated from females admitted for treatment due to male infertility and females diagnosed with reproductive pathologies, who are preparing for assisted reproductive technologies procedures. Using real-time polymerase chain reaction method, we demonstrated that endometrium of females with reproductive pathology has significantly upregulated decidualization related genes HAND2, MUC1, CSF2, increased expression of angiogenesis related gene PDGFA, and increases of overall immune response and inflammation-related genes expression with significant changes of RELA and CXCL10 genes expression. Females with reproductive pathology have altered endometrium epigenetic regulation since expression of miRNAs—specifically, miRNA-34a, miRNA-223, and miRNA-125b—is lower in endometrium of females with reproductive pathology. Our findings suggest that the potential changes in genetic and epigenetic profile of endometrium from females with reproductive pathology could enrich the knowledge in the field of core biological knowledge and treatment of reproductive impairments.
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spelling pubmed-92198392022-06-24 Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility Skliutė, Giedrė Baušytė, Raminta Ramašauskaitė, Diana Navakauskienė, Rūta Biomedicines Article Infertility is one of the most rapidly increasing global health concerns of the 21st century. Embryo quality and endometrial thickness and receptivity are the main factors for successful embryo implantation and pregnancy development. Nevertheless, until now, there has been a lack of understanding about the regulation of human endometrium function and its structure. This raises the demand for more research of the human endometrium in these fields. In our study, we analyzed the genetic and epigenetic changes of endometrial tissue’s samples isolated from females admitted for treatment due to male infertility and females diagnosed with reproductive pathologies, who are preparing for assisted reproductive technologies procedures. Using real-time polymerase chain reaction method, we demonstrated that endometrium of females with reproductive pathology has significantly upregulated decidualization related genes HAND2, MUC1, CSF2, increased expression of angiogenesis related gene PDGFA, and increases of overall immune response and inflammation-related genes expression with significant changes of RELA and CXCL10 genes expression. Females with reproductive pathology have altered endometrium epigenetic regulation since expression of miRNAs—specifically, miRNA-34a, miRNA-223, and miRNA-125b—is lower in endometrium of females with reproductive pathology. Our findings suggest that the potential changes in genetic and epigenetic profile of endometrium from females with reproductive pathology could enrich the knowledge in the field of core biological knowledge and treatment of reproductive impairments. MDPI 2022-06-04 /pmc/articles/PMC9219839/ /pubmed/35740346 http://dx.doi.org/10.3390/biomedicines10061324 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skliutė, Giedrė
Baušytė, Raminta
Ramašauskaitė, Diana
Navakauskienė, Rūta
Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility
title Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility
title_full Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility
title_fullStr Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility
title_full_unstemmed Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility
title_short Characterization of Epigenetic and Molecular Factors in Endometrium of Females with Infertility
title_sort characterization of epigenetic and molecular factors in endometrium of females with infertility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219839/
https://www.ncbi.nlm.nih.gov/pubmed/35740346
http://dx.doi.org/10.3390/biomedicines10061324
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