Cargando…
Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts
Interest in plant extracts as a natural source of antioxidants has grown significantly in recent years. The tree species Koelreuteria paniculata deserves attention due to its wide distribution, good adaptability, and growth to the degree of invasiveness in a number of European countries. The purpose...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219871/ https://www.ncbi.nlm.nih.gov/pubmed/35740051 http://dx.doi.org/10.3390/antiox11061154 |
_version_ | 1784732228439244800 |
---|---|
author | Andonova, Tsvetelina Muhovski, Yordan Vrancheva, Radka Slavov, Ilya Apostolova, Elena Naimov, Samir Pavlov, Atanas Dimitrova-Dyulgerova, Ivanka |
author_facet | Andonova, Tsvetelina Muhovski, Yordan Vrancheva, Radka Slavov, Ilya Apostolova, Elena Naimov, Samir Pavlov, Atanas Dimitrova-Dyulgerova, Ivanka |
author_sort | Andonova, Tsvetelina |
collection | PubMed |
description | Interest in plant extracts as a natural source of antioxidants has grown significantly in recent years. The tree species Koelreuteria paniculata deserves attention due to its wide distribution, good adaptability, and growth to the degree of invasiveness in a number of European countries. The purpose of the present study was to analyze flavonoids and phenolic acids of the ethanol extracts from aerial parts of K. paniculata and to screen their antioxidant and DNA-protective activity. HPLC profiling revealed the presence of five flavonoids, with rutin (4.23 mg/g DW), hesperidin (2.97 mg/g DW), and quercetin (2.66 mg/g DW) as the major ones in the leaves, and (−)-epicatechin (2.69 mg/g DW) in the flower buds. Among the nine phenolic acids identified, rosmarinic, p-coumaric, salicylic, vanillic, and gallic acids were the best represented. All the extracts tested showed in vitro antioxidant activity that was determined by DPPH, ABTS, FRAP, and CUPRAC assays. The highest activity was recorded in the flower parts (in the range from 1133 to 4308 mmol TE/g DW). The DNA-protective capacity of the flower and stem bark extracts from the in vitro nicking assay performed, as well as the main diagnostic microscopic features of the plant substances, are given for the first time. According to the results obtained, the aerial parts of K. paniculata could be valuable sources of natural antioxidants. |
format | Online Article Text |
id | pubmed-9219871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92198712022-06-24 Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts Andonova, Tsvetelina Muhovski, Yordan Vrancheva, Radka Slavov, Ilya Apostolova, Elena Naimov, Samir Pavlov, Atanas Dimitrova-Dyulgerova, Ivanka Antioxidants (Basel) Article Interest in plant extracts as a natural source of antioxidants has grown significantly in recent years. The tree species Koelreuteria paniculata deserves attention due to its wide distribution, good adaptability, and growth to the degree of invasiveness in a number of European countries. The purpose of the present study was to analyze flavonoids and phenolic acids of the ethanol extracts from aerial parts of K. paniculata and to screen their antioxidant and DNA-protective activity. HPLC profiling revealed the presence of five flavonoids, with rutin (4.23 mg/g DW), hesperidin (2.97 mg/g DW), and quercetin (2.66 mg/g DW) as the major ones in the leaves, and (−)-epicatechin (2.69 mg/g DW) in the flower buds. Among the nine phenolic acids identified, rosmarinic, p-coumaric, salicylic, vanillic, and gallic acids were the best represented. All the extracts tested showed in vitro antioxidant activity that was determined by DPPH, ABTS, FRAP, and CUPRAC assays. The highest activity was recorded in the flower parts (in the range from 1133 to 4308 mmol TE/g DW). The DNA-protective capacity of the flower and stem bark extracts from the in vitro nicking assay performed, as well as the main diagnostic microscopic features of the plant substances, are given for the first time. According to the results obtained, the aerial parts of K. paniculata could be valuable sources of natural antioxidants. MDPI 2022-06-13 /pmc/articles/PMC9219871/ /pubmed/35740051 http://dx.doi.org/10.3390/antiox11061154 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Andonova, Tsvetelina Muhovski, Yordan Vrancheva, Radka Slavov, Ilya Apostolova, Elena Naimov, Samir Pavlov, Atanas Dimitrova-Dyulgerova, Ivanka Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts |
title | Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts |
title_full | Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts |
title_fullStr | Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts |
title_full_unstemmed | Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts |
title_short | Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts |
title_sort | antioxidant and dna-protective potentials, main phenolic compounds, and microscopic features of koelreuteria paniculata aerial parts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219871/ https://www.ncbi.nlm.nih.gov/pubmed/35740051 http://dx.doi.org/10.3390/antiox11061154 |
work_keys_str_mv | AT andonovatsvetelina antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts AT muhovskiyordan antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts AT vranchevaradka antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts AT slavovilya antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts AT apostolovaelena antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts AT naimovsamir antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts AT pavlovatanas antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts AT dimitrovadyulgerovaivanka antioxidantanddnaprotectivepotentialsmainphenoliccompoundsandmicroscopicfeaturesofkoelreuteriapaniculataaerialparts |