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Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts

Interest in plant extracts as a natural source of antioxidants has grown significantly in recent years. The tree species Koelreuteria paniculata deserves attention due to its wide distribution, good adaptability, and growth to the degree of invasiveness in a number of European countries. The purpose...

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Autores principales: Andonova, Tsvetelina, Muhovski, Yordan, Vrancheva, Radka, Slavov, Ilya, Apostolova, Elena, Naimov, Samir, Pavlov, Atanas, Dimitrova-Dyulgerova, Ivanka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219871/
https://www.ncbi.nlm.nih.gov/pubmed/35740051
http://dx.doi.org/10.3390/antiox11061154
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author Andonova, Tsvetelina
Muhovski, Yordan
Vrancheva, Radka
Slavov, Ilya
Apostolova, Elena
Naimov, Samir
Pavlov, Atanas
Dimitrova-Dyulgerova, Ivanka
author_facet Andonova, Tsvetelina
Muhovski, Yordan
Vrancheva, Radka
Slavov, Ilya
Apostolova, Elena
Naimov, Samir
Pavlov, Atanas
Dimitrova-Dyulgerova, Ivanka
author_sort Andonova, Tsvetelina
collection PubMed
description Interest in plant extracts as a natural source of antioxidants has grown significantly in recent years. The tree species Koelreuteria paniculata deserves attention due to its wide distribution, good adaptability, and growth to the degree of invasiveness in a number of European countries. The purpose of the present study was to analyze flavonoids and phenolic acids of the ethanol extracts from aerial parts of K. paniculata and to screen their antioxidant and DNA-protective activity. HPLC profiling revealed the presence of five flavonoids, with rutin (4.23 mg/g DW), hesperidin (2.97 mg/g DW), and quercetin (2.66 mg/g DW) as the major ones in the leaves, and (−)-epicatechin (2.69 mg/g DW) in the flower buds. Among the nine phenolic acids identified, rosmarinic, p-coumaric, salicylic, vanillic, and gallic acids were the best represented. All the extracts tested showed in vitro antioxidant activity that was determined by DPPH, ABTS, FRAP, and CUPRAC assays. The highest activity was recorded in the flower parts (in the range from 1133 to 4308 mmol TE/g DW). The DNA-protective capacity of the flower and stem bark extracts from the in vitro nicking assay performed, as well as the main diagnostic microscopic features of the plant substances, are given for the first time. According to the results obtained, the aerial parts of K. paniculata could be valuable sources of natural antioxidants.
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spelling pubmed-92198712022-06-24 Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts Andonova, Tsvetelina Muhovski, Yordan Vrancheva, Radka Slavov, Ilya Apostolova, Elena Naimov, Samir Pavlov, Atanas Dimitrova-Dyulgerova, Ivanka Antioxidants (Basel) Article Interest in plant extracts as a natural source of antioxidants has grown significantly in recent years. The tree species Koelreuteria paniculata deserves attention due to its wide distribution, good adaptability, and growth to the degree of invasiveness in a number of European countries. The purpose of the present study was to analyze flavonoids and phenolic acids of the ethanol extracts from aerial parts of K. paniculata and to screen their antioxidant and DNA-protective activity. HPLC profiling revealed the presence of five flavonoids, with rutin (4.23 mg/g DW), hesperidin (2.97 mg/g DW), and quercetin (2.66 mg/g DW) as the major ones in the leaves, and (−)-epicatechin (2.69 mg/g DW) in the flower buds. Among the nine phenolic acids identified, rosmarinic, p-coumaric, salicylic, vanillic, and gallic acids were the best represented. All the extracts tested showed in vitro antioxidant activity that was determined by DPPH, ABTS, FRAP, and CUPRAC assays. The highest activity was recorded in the flower parts (in the range from 1133 to 4308 mmol TE/g DW). The DNA-protective capacity of the flower and stem bark extracts from the in vitro nicking assay performed, as well as the main diagnostic microscopic features of the plant substances, are given for the first time. According to the results obtained, the aerial parts of K. paniculata could be valuable sources of natural antioxidants. MDPI 2022-06-13 /pmc/articles/PMC9219871/ /pubmed/35740051 http://dx.doi.org/10.3390/antiox11061154 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andonova, Tsvetelina
Muhovski, Yordan
Vrancheva, Radka
Slavov, Ilya
Apostolova, Elena
Naimov, Samir
Pavlov, Atanas
Dimitrova-Dyulgerova, Ivanka
Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts
title Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts
title_full Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts
title_fullStr Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts
title_full_unstemmed Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts
title_short Antioxidant and DNA-Protective Potentials, Main Phenolic Compounds, and Microscopic Features of Koelreuteria paniculata Aerial Parts
title_sort antioxidant and dna-protective potentials, main phenolic compounds, and microscopic features of koelreuteria paniculata aerial parts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219871/
https://www.ncbi.nlm.nih.gov/pubmed/35740051
http://dx.doi.org/10.3390/antiox11061154
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