Cargando…

Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice

Type 2 diabetes mellitus (T2DM) is associated with an oxidative milieu that often leads to adverse health problems. Bioactive peptides of zein possess outstanding antioxidant activity; however, their effects on hyperglycemia-related oxidative stress remain elusive. In the present study, the dipeptid...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhai, Jinghui, Zhu, Yuhua, Wu, Yi, Li, Na, Cao, Yue, Guo, Yi, Xu, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219942/
https://www.ncbi.nlm.nih.gov/pubmed/35740008
http://dx.doi.org/10.3390/antiox11061111
_version_ 1784732247456219136
author Zhai, Jinghui
Zhu, Yuhua
Wu, Yi
Li, Na
Cao, Yue
Guo, Yi
Xu, Li
author_facet Zhai, Jinghui
Zhu, Yuhua
Wu, Yi
Li, Na
Cao, Yue
Guo, Yi
Xu, Li
author_sort Zhai, Jinghui
collection PubMed
description Type 2 diabetes mellitus (T2DM) is associated with an oxidative milieu that often leads to adverse health problems. Bioactive peptides of zein possess outstanding antioxidant activity; however, their effects on hyperglycemia-related oxidative stress remain elusive. In the present study, the dipeptide Tyr-Ala (YA), a functional peptide with typical health benefits, was applied to alleviate oxidative stress in pancreatic islets under hyperglycemic conditions. By detecting viability, antioxidant ability, and insulin secretion in INS-1 cells, YA showed excellent protection of INS-1 cells from H(2)O(2) oxidative stress, erasing reactive oxygen species (ROS) and promoting insulin secretion. Moreover, by Western blotting, we found that YA can regulate the PI3K/Akt signaling pathway associated with glycometabolism. After establishing a T2DM mice model, we treated mice with YA and measured glucose, insulin, hemoglobin A1C (HbA1c), total cholesterol (TC), triglyceride (TG), and malonaldehyde (MDA) levels and activities of superoxide dismutase (SOD) and glutathione (GSH) from blood samples. We observed that YA could reduce the production of glucose, insulin, HbA1c, TC, TG, and MDA, in addition to enhancing the activities of SOD and GSH. YA could also repair the function of the kidneys and pancreas of T2DM mice. Along with the decline in fasting blood glucose, the oxidative stress in islets was alleviated in T2DM mice after YA administration. This may improve the health situation of diabetic patients in the future.
format Online
Article
Text
id pubmed-9219942
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92199422022-06-24 Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice Zhai, Jinghui Zhu, Yuhua Wu, Yi Li, Na Cao, Yue Guo, Yi Xu, Li Antioxidants (Basel) Article Type 2 diabetes mellitus (T2DM) is associated with an oxidative milieu that often leads to adverse health problems. Bioactive peptides of zein possess outstanding antioxidant activity; however, their effects on hyperglycemia-related oxidative stress remain elusive. In the present study, the dipeptide Tyr-Ala (YA), a functional peptide with typical health benefits, was applied to alleviate oxidative stress in pancreatic islets under hyperglycemic conditions. By detecting viability, antioxidant ability, and insulin secretion in INS-1 cells, YA showed excellent protection of INS-1 cells from H(2)O(2) oxidative stress, erasing reactive oxygen species (ROS) and promoting insulin secretion. Moreover, by Western blotting, we found that YA can regulate the PI3K/Akt signaling pathway associated with glycometabolism. After establishing a T2DM mice model, we treated mice with YA and measured glucose, insulin, hemoglobin A1C (HbA1c), total cholesterol (TC), triglyceride (TG), and malonaldehyde (MDA) levels and activities of superoxide dismutase (SOD) and glutathione (GSH) from blood samples. We observed that YA could reduce the production of glucose, insulin, HbA1c, TC, TG, and MDA, in addition to enhancing the activities of SOD and GSH. YA could also repair the function of the kidneys and pancreas of T2DM mice. Along with the decline in fasting blood glucose, the oxidative stress in islets was alleviated in T2DM mice after YA administration. This may improve the health situation of diabetic patients in the future. MDPI 2022-06-02 /pmc/articles/PMC9219942/ /pubmed/35740008 http://dx.doi.org/10.3390/antiox11061111 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhai, Jinghui
Zhu, Yuhua
Wu, Yi
Li, Na
Cao, Yue
Guo, Yi
Xu, Li
Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice
title Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice
title_full Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice
title_fullStr Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice
title_full_unstemmed Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice
title_short Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice
title_sort antioxidant effect of tyr-ala extracted from zein on ins-1 cells and type 2 diabetes high-fat-diet-induced mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219942/
https://www.ncbi.nlm.nih.gov/pubmed/35740008
http://dx.doi.org/10.3390/antiox11061111
work_keys_str_mv AT zhaijinghui antioxidanteffectoftyralaextractedfromzeinonins1cellsandtype2diabeteshighfatdietinducedmice
AT zhuyuhua antioxidanteffectoftyralaextractedfromzeinonins1cellsandtype2diabeteshighfatdietinducedmice
AT wuyi antioxidanteffectoftyralaextractedfromzeinonins1cellsandtype2diabeteshighfatdietinducedmice
AT lina antioxidanteffectoftyralaextractedfromzeinonins1cellsandtype2diabeteshighfatdietinducedmice
AT caoyue antioxidanteffectoftyralaextractedfromzeinonins1cellsandtype2diabeteshighfatdietinducedmice
AT guoyi antioxidanteffectoftyralaextractedfromzeinonins1cellsandtype2diabeteshighfatdietinducedmice
AT xuli antioxidanteffectoftyralaextractedfromzeinonins1cellsandtype2diabeteshighfatdietinducedmice