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Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso

The emergence of antimicrobial-resistant bacteria in developing countries increases risks to the health of both such countries’ residents and the global community due to international travel. It is consequently necessary to investigate antimicrobial-resistant pathogens in countries such as Burkina F...

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Autores principales: Kagambèga, Assèta, McMillan, Elizabeth A., Bouda, Soutongnooma C., Hiott, Lari M., Ramadan, Hazem, Soro, Daniel K., Sharma, Poonam, Gupta, Sushim K., Barro, Nicolas, Jackson, Charlene R., Frye, Jonathan G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219948/
https://www.ncbi.nlm.nih.gov/pubmed/35740187
http://dx.doi.org/10.3390/antibiotics11060782
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author Kagambèga, Assèta
McMillan, Elizabeth A.
Bouda, Soutongnooma C.
Hiott, Lari M.
Ramadan, Hazem
Soro, Daniel K.
Sharma, Poonam
Gupta, Sushim K.
Barro, Nicolas
Jackson, Charlene R.
Frye, Jonathan G.
author_facet Kagambèga, Assèta
McMillan, Elizabeth A.
Bouda, Soutongnooma C.
Hiott, Lari M.
Ramadan, Hazem
Soro, Daniel K.
Sharma, Poonam
Gupta, Sushim K.
Barro, Nicolas
Jackson, Charlene R.
Frye, Jonathan G.
author_sort Kagambèga, Assèta
collection PubMed
description The emergence of antimicrobial-resistant bacteria in developing countries increases risks to the health of both such countries’ residents and the global community due to international travel. It is consequently necessary to investigate antimicrobial-resistant pathogens in countries such as Burkina Faso, where surveillance data are not available. To study the epidemiology of antibiotic resistance in Salmonella, 102 Salmonella strains isolated from slaughtered chickens were subjected to whole-genome sequencing (WGS) to obtain information on antimicrobial resistance (AMR) genes and other genetic factors. Twenty-two different serotypes were identified using WGS, the most prevalent of which were Hato (28/102, 27.5%) and Derby (23/102, 22.5%). All strains analyzed possessed at least one and up to nine AMR genes, with the most prevalent being the non-functional aac(6′)-Iaa gene, followed by aph(6)-Id. Multi-drug resistance was found genotypically in 36.2% of the isolates for different classes of antibiotics, such as fosfomycin and β-lactams, among others. Plasmids were identified in 43.1% of isolates (44/102), and 25 plasmids were confirmed to carry AMR genes. The results show that chicken can be considered as a reservoir of antibiotic-resistant Salmonella strains. Due to the prevalence of these drug-resistant pathogens and the potential for foodborne illnesses, poultry processing and cooking should be performed with attention to prescribed safe handling methods to avoid cross-contamination with chicken products.
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spelling pubmed-92199482022-06-24 Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso Kagambèga, Assèta McMillan, Elizabeth A. Bouda, Soutongnooma C. Hiott, Lari M. Ramadan, Hazem Soro, Daniel K. Sharma, Poonam Gupta, Sushim K. Barro, Nicolas Jackson, Charlene R. Frye, Jonathan G. Antibiotics (Basel) Article The emergence of antimicrobial-resistant bacteria in developing countries increases risks to the health of both such countries’ residents and the global community due to international travel. It is consequently necessary to investigate antimicrobial-resistant pathogens in countries such as Burkina Faso, where surveillance data are not available. To study the epidemiology of antibiotic resistance in Salmonella, 102 Salmonella strains isolated from slaughtered chickens were subjected to whole-genome sequencing (WGS) to obtain information on antimicrobial resistance (AMR) genes and other genetic factors. Twenty-two different serotypes were identified using WGS, the most prevalent of which were Hato (28/102, 27.5%) and Derby (23/102, 22.5%). All strains analyzed possessed at least one and up to nine AMR genes, with the most prevalent being the non-functional aac(6′)-Iaa gene, followed by aph(6)-Id. Multi-drug resistance was found genotypically in 36.2% of the isolates for different classes of antibiotics, such as fosfomycin and β-lactams, among others. Plasmids were identified in 43.1% of isolates (44/102), and 25 plasmids were confirmed to carry AMR genes. The results show that chicken can be considered as a reservoir of antibiotic-resistant Salmonella strains. Due to the prevalence of these drug-resistant pathogens and the potential for foodborne illnesses, poultry processing and cooking should be performed with attention to prescribed safe handling methods to avoid cross-contamination with chicken products. MDPI 2022-06-08 /pmc/articles/PMC9219948/ /pubmed/35740187 http://dx.doi.org/10.3390/antibiotics11060782 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kagambèga, Assèta
McMillan, Elizabeth A.
Bouda, Soutongnooma C.
Hiott, Lari M.
Ramadan, Hazem
Soro, Daniel K.
Sharma, Poonam
Gupta, Sushim K.
Barro, Nicolas
Jackson, Charlene R.
Frye, Jonathan G.
Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso
title Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso
title_full Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso
title_fullStr Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso
title_full_unstemmed Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso
title_short Resistance Genes, Plasmids, Multilocus Sequence Typing (MLST), and Phenotypic Resistance of Non-Typhoidal Salmonella (NTS) Isolated from Slaughtered Chickens in Burkina Faso
title_sort resistance genes, plasmids, multilocus sequence typing (mlst), and phenotypic resistance of non-typhoidal salmonella (nts) isolated from slaughtered chickens in burkina faso
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219948/
https://www.ncbi.nlm.nih.gov/pubmed/35740187
http://dx.doi.org/10.3390/antibiotics11060782
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