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A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli

Bacteria within biofilms may be exposed to sub-minimum inhibitory concentrations (sub-MICs) of antibiotics. Cell-to-cell contact within biofilms facilitates horizontal gene transfers and favors induction of the SOS response. Altogether, it participates in the emergence of antibiotic resistance. Amin...

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Autores principales: Babosan, Anamaria, Gaschet, Margaux, Muggeo, Anaëlle, Jové, Thomas, Skurnik, David, Ploy, Marie-Cécile, de Champs, Christophe, Reffuveille, Fany, Guillard, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220102/
https://www.ncbi.nlm.nih.gov/pubmed/35740122
http://dx.doi.org/10.3390/antibiotics11060715
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author Babosan, Anamaria
Gaschet, Margaux
Muggeo, Anaëlle
Jové, Thomas
Skurnik, David
Ploy, Marie-Cécile
de Champs, Christophe
Reffuveille, Fany
Guillard, Thomas
author_facet Babosan, Anamaria
Gaschet, Margaux
Muggeo, Anaëlle
Jové, Thomas
Skurnik, David
Ploy, Marie-Cécile
de Champs, Christophe
Reffuveille, Fany
Guillard, Thomas
author_sort Babosan, Anamaria
collection PubMed
description Bacteria within biofilms may be exposed to sub-minimum inhibitory concentrations (sub-MICs) of antibiotics. Cell-to-cell contact within biofilms facilitates horizontal gene transfers and favors induction of the SOS response. Altogether, it participates in the emergence of antibiotic resistance. Aminoglycosides at sub-MICs can induce the SOS response through NO accumulation in E. coli carrying the small plasmid with the quinolone resistance qnrD gene (pDIJ09-518a). In this study, we show that in E. coli pDIJ09-518a, the SOS response triggered by sub-MICs of aminoglycosides has important consequences, promoting genetic rearrangement in class 1 integrons and biofilm formation. We found that the integrase expression was increased in E. coli carrying pDIJ09-518a in the presence of tobramycin, which was not observed for the WT isogenic strain that did not carry the qnrD-plasmid. Moreover, we showed that biofilm production was significantly increased in E. coli WT/pDIJ09-518a compared to the WT strain. However, such a higher production was decreased when the Hmp-NO detoxification pathway was fully functional by overexpressing Hmp. Our results showing that a qnrD-plasmid can promote biofilm formation in E. coli and potentiate the acquisition and spread of resistance determinants for other antibiotics complicate the attempts to counteract antibiotic resistance and prevention of biofilm development even further. We anticipate that our findings emphasize the complex challenges that will impact the decisions about antibiotic stewardship, and other decisions related to retaining antibiotics as effective drugs and the development of new drugs.
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spelling pubmed-92201022022-06-24 A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli Babosan, Anamaria Gaschet, Margaux Muggeo, Anaëlle Jové, Thomas Skurnik, David Ploy, Marie-Cécile de Champs, Christophe Reffuveille, Fany Guillard, Thomas Antibiotics (Basel) Article Bacteria within biofilms may be exposed to sub-minimum inhibitory concentrations (sub-MICs) of antibiotics. Cell-to-cell contact within biofilms facilitates horizontal gene transfers and favors induction of the SOS response. Altogether, it participates in the emergence of antibiotic resistance. Aminoglycosides at sub-MICs can induce the SOS response through NO accumulation in E. coli carrying the small plasmid with the quinolone resistance qnrD gene (pDIJ09-518a). In this study, we show that in E. coli pDIJ09-518a, the SOS response triggered by sub-MICs of aminoglycosides has important consequences, promoting genetic rearrangement in class 1 integrons and biofilm formation. We found that the integrase expression was increased in E. coli carrying pDIJ09-518a in the presence of tobramycin, which was not observed for the WT isogenic strain that did not carry the qnrD-plasmid. Moreover, we showed that biofilm production was significantly increased in E. coli WT/pDIJ09-518a compared to the WT strain. However, such a higher production was decreased when the Hmp-NO detoxification pathway was fully functional by overexpressing Hmp. Our results showing that a qnrD-plasmid can promote biofilm formation in E. coli and potentiate the acquisition and spread of resistance determinants for other antibiotics complicate the attempts to counteract antibiotic resistance and prevention of biofilm development even further. We anticipate that our findings emphasize the complex challenges that will impact the decisions about antibiotic stewardship, and other decisions related to retaining antibiotics as effective drugs and the development of new drugs. MDPI 2022-05-26 /pmc/articles/PMC9220102/ /pubmed/35740122 http://dx.doi.org/10.3390/antibiotics11060715 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Babosan, Anamaria
Gaschet, Margaux
Muggeo, Anaëlle
Jové, Thomas
Skurnik, David
Ploy, Marie-Cécile
de Champs, Christophe
Reffuveille, Fany
Guillard, Thomas
A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli
title A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli
title_full A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli
title_fullStr A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli
title_full_unstemmed A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli
title_short A qnrD-Plasmid Promotes Biofilm Formation and Class 1 Integron Gene Cassette Rearrangements in Escherichia coli
title_sort qnrd-plasmid promotes biofilm formation and class 1 integron gene cassette rearrangements in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220102/
https://www.ncbi.nlm.nih.gov/pubmed/35740122
http://dx.doi.org/10.3390/antibiotics11060715
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