Reproductive Biology of Dry Grassland Specialist Ranunculus illyricus L. and Its Implications for Conservation

SIMPLE SUMMARY: The Ranunculus illyricus—Illyrian buttercup—is threatened with extinction in many countries and measures should be taken to protect it. In order to increase the effectiveness of such measures, it is necessary to know the methods of propagation and to evaluate their efficiency. R. illyricus reproduces generatively by seed and vegetatively by clusters of progeny tubers. The method and potential of vegetative propagation are described here for the first time and compared with the potential and actual effectiveness of generative propagation. Both the generative and vegetative propagation methods should be used to strengthen existing populations and create replacements. ABSTRACT: Ranunculus illyricus, a component of xerothermic grasslands, is a declining species and deserves active conservation treatments in many countries preceded by studies on the biology of its reproduction. So far, our knowledge of R. illyricus, a species with two modes of reproduction, has been fragmentary. The purpose of the studies presented here was to describe the annual development cycle of R. illyricus with particular emphasis on the production of underground tuber clusters that serve as vegetative propagation. Based on three-year-long observations in an ex situ collection, the efficiency of vegetative propagation was estimated and compared with the efficiency of generative propagation. It was found that in 3 years the best clones could produce up to 57 progeny clusters followed by flowering specimens in the first season. Meanwhile, the high potential for generative reproduction was suppressed by many limitations including fruit setting, the germination capacity of seeds, seedling survival rate, and additionally, the first flowering plant was observed only in the third year. It seems that the efficiency of vegetative propagation of this species can be higher than the efficiency of generative propagation. Moreover, vegets bloomed in the first year after emergence, whereas the first plant of generative origin was observed to bloom only after 3 years. A large proportion of individuals of vegetative origin can negatively affect the genetic diversity of the population but their survival rate against competing plants is higher. To enhance the existing populations or to create new ones, it would be best to use plants derived from clonal propagation of genets carried out in ex situ conditions.