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Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat

This study investigated the vasoactive effects of des-aspartate-angiotensin-I (DAA-I) in male Wistar rats on whole body vascular bed, isolated perfused kidneys, and aortic rings. Dose–response curves to DAA-I were compared with those to angiotensin II (Ang II). The Ang II-type-1 (AT1) receptor block...

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Autores principales: Wangensteen, Rosemary, Gómez-Guzmán, Manuel, Banegas, Inmaculada, Rodríguez-Gómez, Isabel, Jiménez, Rosario, Duarte, Juan, García-Estañ, Joaquín, Vargas, Félix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220223/
https://www.ncbi.nlm.nih.gov/pubmed/35740253
http://dx.doi.org/10.3390/biomedicines10061230
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author Wangensteen, Rosemary
Gómez-Guzmán, Manuel
Banegas, Inmaculada
Rodríguez-Gómez, Isabel
Jiménez, Rosario
Duarte, Juan
García-Estañ, Joaquín
Vargas, Félix
author_facet Wangensteen, Rosemary
Gómez-Guzmán, Manuel
Banegas, Inmaculada
Rodríguez-Gómez, Isabel
Jiménez, Rosario
Duarte, Juan
García-Estañ, Joaquín
Vargas, Félix
author_sort Wangensteen, Rosemary
collection PubMed
description This study investigated the vasoactive effects of des-aspartate-angiotensin-I (DAA-I) in male Wistar rats on whole body vascular bed, isolated perfused kidneys, and aortic rings. Dose–response curves to DAA-I were compared with those to angiotensin II (Ang II). The Ang II-type-1 (AT1) receptor blocker, losartan, was used to evaluate the role of AT1 receptors in the responses to DAA-I. Studies were also conducted of the responsiveness in aortic rings after endothelium removal, nitric oxide synthase inhibition, or AT2 receptor blockade. DAA-I induced a dose-related systemic pressor response that was shifted to the right compared with Ang II. Losartan markedly attenuated the responsiveness to DAA-I. DAA-I showed a similar pattern in renal vasculature and aortic rings. In aortic rings, removal of endothelium and nitric oxide inhibition increased the sensitivity and maximal response to DAA-I and Ang II. AT2 receptor blockade did not significantly affect the responsiveness to DAA-I. According to these findings, DAA-I increases the systemic blood pressure and vascular tone in conductance and resistance vessels via AT1 receptor activation. This vasoconstrictor effect of DAA-I participates in the homeostatic control of arterial pressure, which can also contribute to the pathogenesis of hypertension. DAA-I may therefore be a potential therapeutic target in cardiovascular disease.
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spelling pubmed-92202232022-06-24 Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat Wangensteen, Rosemary Gómez-Guzmán, Manuel Banegas, Inmaculada Rodríguez-Gómez, Isabel Jiménez, Rosario Duarte, Juan García-Estañ, Joaquín Vargas, Félix Biomedicines Article This study investigated the vasoactive effects of des-aspartate-angiotensin-I (DAA-I) in male Wistar rats on whole body vascular bed, isolated perfused kidneys, and aortic rings. Dose–response curves to DAA-I were compared with those to angiotensin II (Ang II). The Ang II-type-1 (AT1) receptor blocker, losartan, was used to evaluate the role of AT1 receptors in the responses to DAA-I. Studies were also conducted of the responsiveness in aortic rings after endothelium removal, nitric oxide synthase inhibition, or AT2 receptor blockade. DAA-I induced a dose-related systemic pressor response that was shifted to the right compared with Ang II. Losartan markedly attenuated the responsiveness to DAA-I. DAA-I showed a similar pattern in renal vasculature and aortic rings. In aortic rings, removal of endothelium and nitric oxide inhibition increased the sensitivity and maximal response to DAA-I and Ang II. AT2 receptor blockade did not significantly affect the responsiveness to DAA-I. According to these findings, DAA-I increases the systemic blood pressure and vascular tone in conductance and resistance vessels via AT1 receptor activation. This vasoconstrictor effect of DAA-I participates in the homeostatic control of arterial pressure, which can also contribute to the pathogenesis of hypertension. DAA-I may therefore be a potential therapeutic target in cardiovascular disease. MDPI 2022-05-25 /pmc/articles/PMC9220223/ /pubmed/35740253 http://dx.doi.org/10.3390/biomedicines10061230 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wangensteen, Rosemary
Gómez-Guzmán, Manuel
Banegas, Inmaculada
Rodríguez-Gómez, Isabel
Jiménez, Rosario
Duarte, Juan
García-Estañ, Joaquín
Vargas, Félix
Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat
title Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat
title_full Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat
title_fullStr Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat
title_full_unstemmed Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat
title_short Vasoconstrictor and Pressor Effects of Des-Aspartate-Angiotensin I in Rat
title_sort vasoconstrictor and pressor effects of des-aspartate-angiotensin i in rat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220223/
https://www.ncbi.nlm.nih.gov/pubmed/35740253
http://dx.doi.org/10.3390/biomedicines10061230
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