Transcriptome Analysis and GC-MS Profiling of Key Fatty Acid Biosynthesis Genes in Akebia trifoliata (Thunb.) Koidz Seeds

SIMPLE SUMMARY: Plant oil is an important renewable energy substance, and A. trifoliata seeds are of value in this regard. A. trifoliata fruits have many seeds with high oil content, but research progress on A. trifoliata seed oil is slow. Fatty acid biosynthesis is the most important factor affecting plant oil content. Therefore, analysis of the key genes for fatty acid biosynthesis is beneficial for breeding A. trifoliata varieties with high oil content. Here, we report changes in seed oil and key oil biosynthesis genes in the growth period of A. trifoliata based on transcriptome analysis. We found that the development of A. trifoliata seeds and fruits was not synchronized, and when the fruit was ripe, the seed oil content was not the highest. With the development of A. trifoliata seeds, linoleic and oleic acid content was found to decrease and increase, respectively. Subsequently, several key genes for oil biosynthesis in A. trifoliata were identified. These results further our understanding of the mechanism underlying oil biosynthesis in A. trifoliata seeds. ABSTRACT: Akebia trifoliata (Thunb.) Koidz is an important Chinese medicinal and economic crop. Its seeds, which are rich in fatty acids, are usually discarded. As of now, A. trifoliata lipid biosynthesis pathways and genes have not been clearly described. In this work, we found that seed and fruit development of A. trifoliata were not synchronized, and that when the fruit was ripe, seed oil content was not at its highest. As seeds developed, linoleic and oleic acid content was found to decrease and increase, respectively. RNA sequencing yielded 108.45 GB of clean reads from 15 cDNA libraries, containing 8756 differentially expressed genes. We identified 65 unigenes associated with lipid biosynthesis, including fatty acid and triacylglycerol biosynthesis. The 65 unigenes were mapped to the A. trifoliata lipid synthesis pathway. There were 20 AtrFAD family members in A. trifoliata, which could be divided into four sub-groups with the highest number of AtrSADs. Our study revealed the dynamic changes in A. trifoliata seed oil content and composition during its growth period and provides large-scale and comprehensive transcriptome data of A. trifoliata seeds. These findings provide a basis for the improvement of A. trifoliata seed oil yield and quality.