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Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering

Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties. However, their low mechanical properties limit their use in tissue engineering. Herein, to enhance the mechanical properties of...

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Autores principales: Halperin-Sternfeld, Michal, Netanel Liberman, Gal, Kannan, Raha, Netti, Francesca, Ma, Peter X., Arad, Shoshana Malis, Adler-Abramovich, Lihi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220243/
https://www.ncbi.nlm.nih.gov/pubmed/35740409
http://dx.doi.org/10.3390/biomedicines10061388
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author Halperin-Sternfeld, Michal
Netanel Liberman, Gal
Kannan, Raha
Netti, Francesca
Ma, Peter X.
Arad, Shoshana Malis
Adler-Abramovich, Lihi
author_facet Halperin-Sternfeld, Michal
Netanel Liberman, Gal
Kannan, Raha
Netti, Francesca
Ma, Peter X.
Arad, Shoshana Malis
Adler-Abramovich, Lihi
author_sort Halperin-Sternfeld, Michal
collection PubMed
description Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties. However, their low mechanical properties limit their use in tissue engineering. Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp. (PS), it was integrated with the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator. Transparent, stable hydrogels were formed when mixing the two components at a 1:1 ratio in three different concentrations. Electron microscopy showed that all hydrogels exhibited a nanofibrous structure, mimicking the extracellular matrix. Furthermore, the hydrogels were injectable, and tunable mechanical properties were obtained by changing the hydrogel concentration. The composite hydrogels allowed the sustained release of curcumin which was controlled by the change in the hydrogel concentration. Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition. The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
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spelling pubmed-92202432022-06-24 Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering Halperin-Sternfeld, Michal Netanel Liberman, Gal Kannan, Raha Netti, Francesca Ma, Peter X. Arad, Shoshana Malis Adler-Abramovich, Lihi Biomedicines Article Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties. However, their low mechanical properties limit their use in tissue engineering. Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp. (PS), it was integrated with the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator. Transparent, stable hydrogels were formed when mixing the two components at a 1:1 ratio in three different concentrations. Electron microscopy showed that all hydrogels exhibited a nanofibrous structure, mimicking the extracellular matrix. Furthermore, the hydrogels were injectable, and tunable mechanical properties were obtained by changing the hydrogel concentration. The composite hydrogels allowed the sustained release of curcumin which was controlled by the change in the hydrogel concentration. Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition. The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages. MDPI 2022-06-11 /pmc/articles/PMC9220243/ /pubmed/35740409 http://dx.doi.org/10.3390/biomedicines10061388 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Halperin-Sternfeld, Michal
Netanel Liberman, Gal
Kannan, Raha
Netti, Francesca
Ma, Peter X.
Arad, Shoshana Malis
Adler-Abramovich, Lihi
Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering
title Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering
title_full Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering
title_fullStr Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering
title_full_unstemmed Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering
title_short Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering
title_sort thixotropic red microalgae sulfated polysaccharide-peptide composite hydrogels as scaffolds for tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220243/
https://www.ncbi.nlm.nih.gov/pubmed/35740409
http://dx.doi.org/10.3390/biomedicines10061388
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