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Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model

To test probiotic therapy for osteoarthritis (OA), we administered Lactobacillus acidophilus (LA) by oral gavage (2×/week) after induction of OA by partial medial meniscectomy (PMM). Pain was assessed by von Frey filament and hot plate testing. Joint pathology and pain markers were comprehensively a...

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Autores principales: O-Sullivan, InSug, Natarajan Anbazhagan, Arivarasu, Singh, Gurjit, Ma, Kaige, Green, Stefan J., Singhal, Megha, Wang, Jun, Kumar, Anoop, Dudeja, Pradeep K., Unterman, Terry G., Votta-Velis, Gina, Bruce, Benjamin, van Wijnen, Andre J., Im, Hee-Jeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220766/
https://www.ncbi.nlm.nih.gov/pubmed/35740320
http://dx.doi.org/10.3390/biomedicines10061298
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author O-Sullivan, InSug
Natarajan Anbazhagan, Arivarasu
Singh, Gurjit
Ma, Kaige
Green, Stefan J.
Singhal, Megha
Wang, Jun
Kumar, Anoop
Dudeja, Pradeep K.
Unterman, Terry G.
Votta-Velis, Gina
Bruce, Benjamin
van Wijnen, Andre J.
Im, Hee-Jeong
author_facet O-Sullivan, InSug
Natarajan Anbazhagan, Arivarasu
Singh, Gurjit
Ma, Kaige
Green, Stefan J.
Singhal, Megha
Wang, Jun
Kumar, Anoop
Dudeja, Pradeep K.
Unterman, Terry G.
Votta-Velis, Gina
Bruce, Benjamin
van Wijnen, Andre J.
Im, Hee-Jeong
author_sort O-Sullivan, InSug
collection PubMed
description To test probiotic therapy for osteoarthritis (OA), we administered Lactobacillus acidophilus (LA) by oral gavage (2×/week) after induction of OA by partial medial meniscectomy (PMM). Pain was assessed by von Frey filament and hot plate testing. Joint pathology and pain markers were comprehensively analyzed in knee joints, spinal cords, dorsal root ganglia and distal colon by Safranin O/fast green staining, immunofluorescence microscopy and RT-qPCR. LA acutely reduced inflammatory knee joint pain and prevented further OA progression. The therapeutic efficacy of LA was supported by a significant reduction of cartilage-degrading enzymes, pain markers and inflammatory factors in the tissues we examined. This finding suggests a likely clinical effect of LA on OA. The effect of LA treatment on the fecal microbiome was assessed by 16S rRNA gene amplicon sequencing analysis. LA significantly altered the fecal microbiota compared to vehicle-treated mice (PERMANOVA p < 0.009). Our pre-clinical OA animal model revealed significant OA disease modifying effects of LA as reflected by rapid joint pain reduction, cartilage protection, and reversal of dysbiosis. Our findings suggest that LA treatment has beneficial systemic effects that can potentially be developed as a safe OA disease-modifying drug (OADMD).
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spelling pubmed-92207662022-06-24 Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model O-Sullivan, InSug Natarajan Anbazhagan, Arivarasu Singh, Gurjit Ma, Kaige Green, Stefan J. Singhal, Megha Wang, Jun Kumar, Anoop Dudeja, Pradeep K. Unterman, Terry G. Votta-Velis, Gina Bruce, Benjamin van Wijnen, Andre J. Im, Hee-Jeong Biomedicines Article To test probiotic therapy for osteoarthritis (OA), we administered Lactobacillus acidophilus (LA) by oral gavage (2×/week) after induction of OA by partial medial meniscectomy (PMM). Pain was assessed by von Frey filament and hot plate testing. Joint pathology and pain markers were comprehensively analyzed in knee joints, spinal cords, dorsal root ganglia and distal colon by Safranin O/fast green staining, immunofluorescence microscopy and RT-qPCR. LA acutely reduced inflammatory knee joint pain and prevented further OA progression. The therapeutic efficacy of LA was supported by a significant reduction of cartilage-degrading enzymes, pain markers and inflammatory factors in the tissues we examined. This finding suggests a likely clinical effect of LA on OA. The effect of LA treatment on the fecal microbiome was assessed by 16S rRNA gene amplicon sequencing analysis. LA significantly altered the fecal microbiota compared to vehicle-treated mice (PERMANOVA p < 0.009). Our pre-clinical OA animal model revealed significant OA disease modifying effects of LA as reflected by rapid joint pain reduction, cartilage protection, and reversal of dysbiosis. Our findings suggest that LA treatment has beneficial systemic effects that can potentially be developed as a safe OA disease-modifying drug (OADMD). MDPI 2022-06-01 /pmc/articles/PMC9220766/ /pubmed/35740320 http://dx.doi.org/10.3390/biomedicines10061298 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
O-Sullivan, InSug
Natarajan Anbazhagan, Arivarasu
Singh, Gurjit
Ma, Kaige
Green, Stefan J.
Singhal, Megha
Wang, Jun
Kumar, Anoop
Dudeja, Pradeep K.
Unterman, Terry G.
Votta-Velis, Gina
Bruce, Benjamin
van Wijnen, Andre J.
Im, Hee-Jeong
Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model
title Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model
title_full Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model
title_fullStr Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model
title_full_unstemmed Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model
title_short Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model
title_sort lactobacillus acidophilus mitigates osteoarthritis-associated pain, cartilage disintegration and gut microbiota dysbiosis in an experimental murine oa model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220766/
https://www.ncbi.nlm.nih.gov/pubmed/35740320
http://dx.doi.org/10.3390/biomedicines10061298
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