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Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model
To test probiotic therapy for osteoarthritis (OA), we administered Lactobacillus acidophilus (LA) by oral gavage (2×/week) after induction of OA by partial medial meniscectomy (PMM). Pain was assessed by von Frey filament and hot plate testing. Joint pathology and pain markers were comprehensively a...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220766/ https://www.ncbi.nlm.nih.gov/pubmed/35740320 http://dx.doi.org/10.3390/biomedicines10061298 |
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author | O-Sullivan, InSug Natarajan Anbazhagan, Arivarasu Singh, Gurjit Ma, Kaige Green, Stefan J. Singhal, Megha Wang, Jun Kumar, Anoop Dudeja, Pradeep K. Unterman, Terry G. Votta-Velis, Gina Bruce, Benjamin van Wijnen, Andre J. Im, Hee-Jeong |
author_facet | O-Sullivan, InSug Natarajan Anbazhagan, Arivarasu Singh, Gurjit Ma, Kaige Green, Stefan J. Singhal, Megha Wang, Jun Kumar, Anoop Dudeja, Pradeep K. Unterman, Terry G. Votta-Velis, Gina Bruce, Benjamin van Wijnen, Andre J. Im, Hee-Jeong |
author_sort | O-Sullivan, InSug |
collection | PubMed |
description | To test probiotic therapy for osteoarthritis (OA), we administered Lactobacillus acidophilus (LA) by oral gavage (2×/week) after induction of OA by partial medial meniscectomy (PMM). Pain was assessed by von Frey filament and hot plate testing. Joint pathology and pain markers were comprehensively analyzed in knee joints, spinal cords, dorsal root ganglia and distal colon by Safranin O/fast green staining, immunofluorescence microscopy and RT-qPCR. LA acutely reduced inflammatory knee joint pain and prevented further OA progression. The therapeutic efficacy of LA was supported by a significant reduction of cartilage-degrading enzymes, pain markers and inflammatory factors in the tissues we examined. This finding suggests a likely clinical effect of LA on OA. The effect of LA treatment on the fecal microbiome was assessed by 16S rRNA gene amplicon sequencing analysis. LA significantly altered the fecal microbiota compared to vehicle-treated mice (PERMANOVA p < 0.009). Our pre-clinical OA animal model revealed significant OA disease modifying effects of LA as reflected by rapid joint pain reduction, cartilage protection, and reversal of dysbiosis. Our findings suggest that LA treatment has beneficial systemic effects that can potentially be developed as a safe OA disease-modifying drug (OADMD). |
format | Online Article Text |
id | pubmed-9220766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92207662022-06-24 Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model O-Sullivan, InSug Natarajan Anbazhagan, Arivarasu Singh, Gurjit Ma, Kaige Green, Stefan J. Singhal, Megha Wang, Jun Kumar, Anoop Dudeja, Pradeep K. Unterman, Terry G. Votta-Velis, Gina Bruce, Benjamin van Wijnen, Andre J. Im, Hee-Jeong Biomedicines Article To test probiotic therapy for osteoarthritis (OA), we administered Lactobacillus acidophilus (LA) by oral gavage (2×/week) after induction of OA by partial medial meniscectomy (PMM). Pain was assessed by von Frey filament and hot plate testing. Joint pathology and pain markers were comprehensively analyzed in knee joints, spinal cords, dorsal root ganglia and distal colon by Safranin O/fast green staining, immunofluorescence microscopy and RT-qPCR. LA acutely reduced inflammatory knee joint pain and prevented further OA progression. The therapeutic efficacy of LA was supported by a significant reduction of cartilage-degrading enzymes, pain markers and inflammatory factors in the tissues we examined. This finding suggests a likely clinical effect of LA on OA. The effect of LA treatment on the fecal microbiome was assessed by 16S rRNA gene amplicon sequencing analysis. LA significantly altered the fecal microbiota compared to vehicle-treated mice (PERMANOVA p < 0.009). Our pre-clinical OA animal model revealed significant OA disease modifying effects of LA as reflected by rapid joint pain reduction, cartilage protection, and reversal of dysbiosis. Our findings suggest that LA treatment has beneficial systemic effects that can potentially be developed as a safe OA disease-modifying drug (OADMD). MDPI 2022-06-01 /pmc/articles/PMC9220766/ /pubmed/35740320 http://dx.doi.org/10.3390/biomedicines10061298 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article O-Sullivan, InSug Natarajan Anbazhagan, Arivarasu Singh, Gurjit Ma, Kaige Green, Stefan J. Singhal, Megha Wang, Jun Kumar, Anoop Dudeja, Pradeep K. Unterman, Terry G. Votta-Velis, Gina Bruce, Benjamin van Wijnen, Andre J. Im, Hee-Jeong Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model |
title | Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model |
title_full | Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model |
title_fullStr | Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model |
title_full_unstemmed | Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model |
title_short | Lactobacillus acidophilus Mitigates Osteoarthritis-Associated Pain, Cartilage Disintegration and Gut Microbiota Dysbiosis in an Experimental Murine OA Model |
title_sort | lactobacillus acidophilus mitigates osteoarthritis-associated pain, cartilage disintegration and gut microbiota dysbiosis in an experimental murine oa model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220766/ https://www.ncbi.nlm.nih.gov/pubmed/35740320 http://dx.doi.org/10.3390/biomedicines10061298 |
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