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Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor

An IoT-WiFi smart and portable electrochemical immunosensor for the quantification of SARS-CoV-2 spike protein was developed with integrated machine learning features. The immunoenzymatic sensor is based on the immobilization of monoclonal antibodies directed at the SARS-CoV-2 S1 subunit on Screen-P...

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Autores principales: Fortunati, Simone, Giliberti, Chiara, Giannetto, Marco, Bolchi, Angelo, Ferrari, Davide, Donofrio, Gaetano, Bianchi, Valentina, Boni, Andrea, De Munari, Ilaria, Careri, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220900/
https://www.ncbi.nlm.nih.gov/pubmed/35735573
http://dx.doi.org/10.3390/bios12060426
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author Fortunati, Simone
Giliberti, Chiara
Giannetto, Marco
Bolchi, Angelo
Ferrari, Davide
Donofrio, Gaetano
Bianchi, Valentina
Boni, Andrea
De Munari, Ilaria
Careri, Maria
author_facet Fortunati, Simone
Giliberti, Chiara
Giannetto, Marco
Bolchi, Angelo
Ferrari, Davide
Donofrio, Gaetano
Bianchi, Valentina
Boni, Andrea
De Munari, Ilaria
Careri, Maria
author_sort Fortunati, Simone
collection PubMed
description An IoT-WiFi smart and portable electrochemical immunosensor for the quantification of SARS-CoV-2 spike protein was developed with integrated machine learning features. The immunoenzymatic sensor is based on the immobilization of monoclonal antibodies directed at the SARS-CoV-2 S1 subunit on Screen-Printed Electrodes functionalized with gold nanoparticles. The analytical protocol involves a single-step sample incubation. Immunosensor performance was validated in a viral transfer medium which is commonly used for the desorption of nasopharyngeal swabs. Remarkable specificity of the response was demonstrated by testing H1N1 Hemagglutinin from swine-origin influenza A virus and Spike Protein S1 from Middle East respiratory syndrome coronavirus. Machine learning was successfully used for data processing and analysis. Different support vector machine classifiers were evaluated, proving that algorithms affect the classifier accuracy. The test accuracy of the best classification model in terms of true positive/true negative sample classification was 97.3%. In addition, the ML algorithm can be easily integrated into cloud-based portable Wi-Fi devices. Finally, the immunosensor was successfully tested using a third generation replicating incompetent lentiviral vector pseudotyped with SARS-CoV-2 spike glycoprotein, thus proving the applicability of the immunosensor to whole virus detection.
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spelling pubmed-92209002022-06-24 Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor Fortunati, Simone Giliberti, Chiara Giannetto, Marco Bolchi, Angelo Ferrari, Davide Donofrio, Gaetano Bianchi, Valentina Boni, Andrea De Munari, Ilaria Careri, Maria Biosensors (Basel) Article An IoT-WiFi smart and portable electrochemical immunosensor for the quantification of SARS-CoV-2 spike protein was developed with integrated machine learning features. The immunoenzymatic sensor is based on the immobilization of monoclonal antibodies directed at the SARS-CoV-2 S1 subunit on Screen-Printed Electrodes functionalized with gold nanoparticles. The analytical protocol involves a single-step sample incubation. Immunosensor performance was validated in a viral transfer medium which is commonly used for the desorption of nasopharyngeal swabs. Remarkable specificity of the response was demonstrated by testing H1N1 Hemagglutinin from swine-origin influenza A virus and Spike Protein S1 from Middle East respiratory syndrome coronavirus. Machine learning was successfully used for data processing and analysis. Different support vector machine classifiers were evaluated, proving that algorithms affect the classifier accuracy. The test accuracy of the best classification model in terms of true positive/true negative sample classification was 97.3%. In addition, the ML algorithm can be easily integrated into cloud-based portable Wi-Fi devices. Finally, the immunosensor was successfully tested using a third generation replicating incompetent lentiviral vector pseudotyped with SARS-CoV-2 spike glycoprotein, thus proving the applicability of the immunosensor to whole virus detection. MDPI 2022-06-17 /pmc/articles/PMC9220900/ /pubmed/35735573 http://dx.doi.org/10.3390/bios12060426 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fortunati, Simone
Giliberti, Chiara
Giannetto, Marco
Bolchi, Angelo
Ferrari, Davide
Donofrio, Gaetano
Bianchi, Valentina
Boni, Andrea
De Munari, Ilaria
Careri, Maria
Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor
title Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor
title_full Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor
title_fullStr Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor
title_full_unstemmed Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor
title_short Rapid Quantification of SARS-Cov-2 Spike Protein Enhanced with a Machine Learning Technique Integrated in a Smart and Portable Immunosensor
title_sort rapid quantification of sars-cov-2 spike protein enhanced with a machine learning technique integrated in a smart and portable immunosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220900/
https://www.ncbi.nlm.nih.gov/pubmed/35735573
http://dx.doi.org/10.3390/bios12060426
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