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Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities
Nanotechnology is an emerging area of research that deals with the production, manipulation, and application of nanoscale materials. Bio-assisted synthesis is of particular interest nowadays, to overcome the limitations associated with the physical and chemical means. The aim of this study was to sy...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221242/ https://www.ncbi.nlm.nih.gov/pubmed/35740980 http://dx.doi.org/10.3390/biom12060855 |
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author | Meer, Bisma Andleeb, Anisa Iqbal, Junaid Ashraf, Hajra Meer, Kushif Ali, Joham Sarfraz Drouet, Samantha Anjum, Sumaira Mehmood, Azra Khan, Taimoor Ali, Mohammad Hano, Christophe Abbasi, Bilal Haider |
author_facet | Meer, Bisma Andleeb, Anisa Iqbal, Junaid Ashraf, Hajra Meer, Kushif Ali, Joham Sarfraz Drouet, Samantha Anjum, Sumaira Mehmood, Azra Khan, Taimoor Ali, Mohammad Hano, Christophe Abbasi, Bilal Haider |
author_sort | Meer, Bisma |
collection | PubMed |
description | Nanotechnology is an emerging area of research that deals with the production, manipulation, and application of nanoscale materials. Bio-assisted synthesis is of particular interest nowadays, to overcome the limitations associated with the physical and chemical means. The aim of this study was to synthesize ZnO nanoparticles (NPs) for the first time, utilizing the seed extract of Lepidium sativum. The synthesized NPs were confirmed through various spectroscopy and imagining techniques, such as XRD, FTIR, HPLC, and SEM. The characterized NPs were then examined for various in vitro biological assays. Crystalline, hexagonal-structured NPs with an average particle size of 25.6 nm were obtained. Biosynthesized ZnO NPs exhibited potent antioxidant activities, effective α-amylase inhibition, moderate urease inhibition (56%), high lipase-inhibition (71%) activities, moderate cytotoxic potential, and significant antibacterial activity. Gene expression of caspase in HepG2 cells was enhanced along with elevated production of ROS/RNS, while membrane integrity was disturbed upon the exposure of NPs. Overall results indicated that bio-assisted ZnO NPs exhibit excellent biological potential and could be exploited for future biomedical applications. particularly in antimicrobial and cancer therapeutics. Moreover, this is the first comprehensive study on Lepidium sativum-mediated synthesis of ZnO nanoparticles and evaluation of their biological activities. |
format | Online Article Text |
id | pubmed-9221242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92212422022-06-24 Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities Meer, Bisma Andleeb, Anisa Iqbal, Junaid Ashraf, Hajra Meer, Kushif Ali, Joham Sarfraz Drouet, Samantha Anjum, Sumaira Mehmood, Azra Khan, Taimoor Ali, Mohammad Hano, Christophe Abbasi, Bilal Haider Biomolecules Article Nanotechnology is an emerging area of research that deals with the production, manipulation, and application of nanoscale materials. Bio-assisted synthesis is of particular interest nowadays, to overcome the limitations associated with the physical and chemical means. The aim of this study was to synthesize ZnO nanoparticles (NPs) for the first time, utilizing the seed extract of Lepidium sativum. The synthesized NPs were confirmed through various spectroscopy and imagining techniques, such as XRD, FTIR, HPLC, and SEM. The characterized NPs were then examined for various in vitro biological assays. Crystalline, hexagonal-structured NPs with an average particle size of 25.6 nm were obtained. Biosynthesized ZnO NPs exhibited potent antioxidant activities, effective α-amylase inhibition, moderate urease inhibition (56%), high lipase-inhibition (71%) activities, moderate cytotoxic potential, and significant antibacterial activity. Gene expression of caspase in HepG2 cells was enhanced along with elevated production of ROS/RNS, while membrane integrity was disturbed upon the exposure of NPs. Overall results indicated that bio-assisted ZnO NPs exhibit excellent biological potential and could be exploited for future biomedical applications. particularly in antimicrobial and cancer therapeutics. Moreover, this is the first comprehensive study on Lepidium sativum-mediated synthesis of ZnO nanoparticles and evaluation of their biological activities. MDPI 2022-06-20 /pmc/articles/PMC9221242/ /pubmed/35740980 http://dx.doi.org/10.3390/biom12060855 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meer, Bisma Andleeb, Anisa Iqbal, Junaid Ashraf, Hajra Meer, Kushif Ali, Joham Sarfraz Drouet, Samantha Anjum, Sumaira Mehmood, Azra Khan, Taimoor Ali, Mohammad Hano, Christophe Abbasi, Bilal Haider Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities |
title | Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities |
title_full | Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities |
title_fullStr | Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities |
title_full_unstemmed | Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities |
title_short | Bio-Assisted Synthesis and Characterization of Zinc Oxide Nanoparticles from Lepidium sativum and Their Potent Antioxidant, Antibacterial and Anticancer Activities |
title_sort | bio-assisted synthesis and characterization of zinc oxide nanoparticles from lepidium sativum and their potent antioxidant, antibacterial and anticancer activities |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221242/ https://www.ncbi.nlm.nih.gov/pubmed/35740980 http://dx.doi.org/10.3390/biom12060855 |
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