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Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets

Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is necessary for the accurate measurement of binding...

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Autores principales: Liang, Runli, Zhang, Yingnan, Ma, Guangzhong, Wang, Shaopeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221260/
https://www.ncbi.nlm.nih.gov/pubmed/35735542
http://dx.doi.org/10.3390/bios12060394
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author Liang, Runli
Zhang, Yingnan
Ma, Guangzhong
Wang, Shaopeng
author_facet Liang, Runli
Zhang, Yingnan
Ma, Guangzhong
Wang, Shaopeng
author_sort Liang, Runli
collection PubMed
description Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is necessary for the accurate measurement of binding kinetics in order to properly triage the best peptide leads during the affinity maturation stages. A sensitive technology is needed for directly measuring the binding kinetics of peptides on phages to reduce the time and cost of the entire process. Here, we show the capability of a charge-sensitive optical detection (CSOD) method for the direct quantification of binding kinetics of phage-displayed peptides to their target protein, using whole phages. We anticipate CSOD will contribute to streamline the process of phage display-based drug discovery.
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spelling pubmed-92212602022-06-24 Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets Liang, Runli Zhang, Yingnan Ma, Guangzhong Wang, Shaopeng Biosensors (Basel) Article Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is necessary for the accurate measurement of binding kinetics in order to properly triage the best peptide leads during the affinity maturation stages. A sensitive technology is needed for directly measuring the binding kinetics of peptides on phages to reduce the time and cost of the entire process. Here, we show the capability of a charge-sensitive optical detection (CSOD) method for the direct quantification of binding kinetics of phage-displayed peptides to their target protein, using whole phages. We anticipate CSOD will contribute to streamline the process of phage display-based drug discovery. MDPI 2022-06-08 /pmc/articles/PMC9221260/ /pubmed/35735542 http://dx.doi.org/10.3390/bios12060394 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liang, Runli
Zhang, Yingnan
Ma, Guangzhong
Wang, Shaopeng
Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets
title Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets
title_full Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets
title_fullStr Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets
title_full_unstemmed Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets
title_short Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets
title_sort charge-sensitive optical detection of binding kinetics between phage-displayed peptide ligands and protein targets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221260/
https://www.ncbi.nlm.nih.gov/pubmed/35735542
http://dx.doi.org/10.3390/bios12060394
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