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Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro

Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence...

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Autores principales: Hromada, Carina, Hartmann, Jaana, Oesterreicher, Johannes, Stoiber, Anton, Daerr, Anna, Schädl, Barbara, Priglinger, Eleni, Teuschl-Woller, Andreas H., Holnthoner, Wolfgang, Heinzel, Johannes, Hercher, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221261/
https://www.ncbi.nlm.nih.gov/pubmed/35740945
http://dx.doi.org/10.3390/biom12060820
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author Hromada, Carina
Hartmann, Jaana
Oesterreicher, Johannes
Stoiber, Anton
Daerr, Anna
Schädl, Barbara
Priglinger, Eleni
Teuschl-Woller, Andreas H.
Holnthoner, Wolfgang
Heinzel, Johannes
Hercher, David
author_facet Hromada, Carina
Hartmann, Jaana
Oesterreicher, Johannes
Stoiber, Anton
Daerr, Anna
Schädl, Barbara
Priglinger, Eleni
Teuschl-Woller, Andreas H.
Holnthoner, Wolfgang
Heinzel, Johannes
Hercher, David
author_sort Hromada, Carina
collection PubMed
description Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly under-investigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes did not influence the respective other cell types’ migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels, in the presence of supporting cells; whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe, for the first time, increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance of elucidating the molecular mechanisms of SC–LEC interaction.
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spelling pubmed-92212612022-06-24 Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro Hromada, Carina Hartmann, Jaana Oesterreicher, Johannes Stoiber, Anton Daerr, Anna Schädl, Barbara Priglinger, Eleni Teuschl-Woller, Andreas H. Holnthoner, Wolfgang Heinzel, Johannes Hercher, David Biomolecules Article Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly under-investigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes did not influence the respective other cell types’ migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels, in the presence of supporting cells; whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe, for the first time, increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance of elucidating the molecular mechanisms of SC–LEC interaction. MDPI 2022-06-12 /pmc/articles/PMC9221261/ /pubmed/35740945 http://dx.doi.org/10.3390/biom12060820 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hromada, Carina
Hartmann, Jaana
Oesterreicher, Johannes
Stoiber, Anton
Daerr, Anna
Schädl, Barbara
Priglinger, Eleni
Teuschl-Woller, Andreas H.
Holnthoner, Wolfgang
Heinzel, Johannes
Hercher, David
Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro
title Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro
title_full Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro
title_fullStr Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro
title_full_unstemmed Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro
title_short Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro
title_sort occurrence of lymphangiogenesis in peripheral nerve autografts contrasts schwann cell-induced apoptosis of lymphatic endothelial cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221261/
https://www.ncbi.nlm.nih.gov/pubmed/35740945
http://dx.doi.org/10.3390/biom12060820
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