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Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination

Bisphenol A (BPA) has emerged as a contaminant of concern because long-term exposure may affect the human endocrine system. Herein, a novel aptamer sensor based on magnetic separation and surface-enhanced Raman scattering (SERS) is proposed for the extremely sensitive and specific detection of trace...

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Autores principales: Li, Sen, He, Defu, Li, Shuning, Chen, Ruipeng, Peng, Yuan, Li, Shuang, Han, Dianpeng, Wang, Yu, Qin, Kang, Ren, Shuyue, Chen, Ping, Gao, Zhixian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221462/
https://www.ncbi.nlm.nih.gov/pubmed/35735535
http://dx.doi.org/10.3390/bios12060387
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author Li, Sen
He, Defu
Li, Shuning
Chen, Ruipeng
Peng, Yuan
Li, Shuang
Han, Dianpeng
Wang, Yu
Qin, Kang
Ren, Shuyue
Chen, Ping
Gao, Zhixian
author_facet Li, Sen
He, Defu
Li, Shuning
Chen, Ruipeng
Peng, Yuan
Li, Shuang
Han, Dianpeng
Wang, Yu
Qin, Kang
Ren, Shuyue
Chen, Ping
Gao, Zhixian
author_sort Li, Sen
collection PubMed
description Bisphenol A (BPA) has emerged as a contaminant of concern because long-term exposure may affect the human endocrine system. Herein, a novel aptamer sensor based on magnetic separation and surface-enhanced Raman scattering (SERS) is proposed for the extremely sensitive and specific detection of trace BPA. Moreover, the capture unit was prepared by immobilizing thiolated (SH)-BPA aptamer complementary DNA on AuNP-coated magnetic halloysite nanotubes (MNTs@AuNPs), and SH-BPA aptamer-modified Au@4-MBA@Ag core–shell SERS nanotags acted as signal units. By the complementary pairing of the BPA aptamer and the corresponding DNA, MNTs@AuNPs and Au@4-MBA@AgCS were linked together through hybridization-ligation, which acted as the SERS substrate. In the absence of BPA, the constructed aptamer sensor generated electromagnetic enhancement and plasmon coupling to improve the sensitivity of SERS substrates. Owing to the high affinity between BPA and the aptamer, the aptamer probe bound to BPA was separated from the capture unit by an externally-induced magnetic field. Thus, the Raman intensity of the MNTs@AuNP-Ag@AuCS core–satellite assemblies was negatively correlated with the BPA concentration. High sensitivity measurements of BPA might be performed by determining the decline in SERS signal strength together with concentration variations. The proposed aptasensor is a promising biosensing platform for BPA detection.
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spelling pubmed-92214622022-06-24 Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination Li, Sen He, Defu Li, Shuning Chen, Ruipeng Peng, Yuan Li, Shuang Han, Dianpeng Wang, Yu Qin, Kang Ren, Shuyue Chen, Ping Gao, Zhixian Biosensors (Basel) Article Bisphenol A (BPA) has emerged as a contaminant of concern because long-term exposure may affect the human endocrine system. Herein, a novel aptamer sensor based on magnetic separation and surface-enhanced Raman scattering (SERS) is proposed for the extremely sensitive and specific detection of trace BPA. Moreover, the capture unit was prepared by immobilizing thiolated (SH)-BPA aptamer complementary DNA on AuNP-coated magnetic halloysite nanotubes (MNTs@AuNPs), and SH-BPA aptamer-modified Au@4-MBA@Ag core–shell SERS nanotags acted as signal units. By the complementary pairing of the BPA aptamer and the corresponding DNA, MNTs@AuNPs and Au@4-MBA@AgCS were linked together through hybridization-ligation, which acted as the SERS substrate. In the absence of BPA, the constructed aptamer sensor generated electromagnetic enhancement and plasmon coupling to improve the sensitivity of SERS substrates. Owing to the high affinity between BPA and the aptamer, the aptamer probe bound to BPA was separated from the capture unit by an externally-induced magnetic field. Thus, the Raman intensity of the MNTs@AuNP-Ag@AuCS core–satellite assemblies was negatively correlated with the BPA concentration. High sensitivity measurements of BPA might be performed by determining the decline in SERS signal strength together with concentration variations. The proposed aptasensor is a promising biosensing platform for BPA detection. MDPI 2022-06-02 /pmc/articles/PMC9221462/ /pubmed/35735535 http://dx.doi.org/10.3390/bios12060387 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Sen
He, Defu
Li, Shuning
Chen, Ruipeng
Peng, Yuan
Li, Shuang
Han, Dianpeng
Wang, Yu
Qin, Kang
Ren, Shuyue
Chen, Ping
Gao, Zhixian
Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination
title Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination
title_full Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination
title_fullStr Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination
title_full_unstemmed Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination
title_short Magnetic Halloysite Nanotube-Based SERS Biosensor Enhanced with Au@Ag Core–Shell Nanotags for Bisphenol A Determination
title_sort magnetic halloysite nanotube-based sers biosensor enhanced with au@ag core–shell nanotags for bisphenol a determination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221462/
https://www.ncbi.nlm.nih.gov/pubmed/35735535
http://dx.doi.org/10.3390/bios12060387
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