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Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models

The extracellular matrix (ECM) is a complex mixture of structural proteins, proteoglycans, and signaling molecules that are essential for tissue integrity and homeostasis. While a number of recent studies have explored the use of decellularized ECM (dECM) as a biomaterial for tissue engineering, the...

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Autores principales: Sarmin, Atiya M., El Moussaid, Nadia, Suntornnond, Ratima, Tyler, Eleanor J., Kim, Yang-Hee, Di Cio, Stefania, Megone, William V., Pearce, Oliver, Gautrot, Julien E., Dawson, Jonathan, Connelly, John T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221483/
https://www.ncbi.nlm.nih.gov/pubmed/35740962
http://dx.doi.org/10.3390/biom12060837
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author Sarmin, Atiya M.
El Moussaid, Nadia
Suntornnond, Ratima
Tyler, Eleanor J.
Kim, Yang-Hee
Di Cio, Stefania
Megone, William V.
Pearce, Oliver
Gautrot, Julien E.
Dawson, Jonathan
Connelly, John T.
author_facet Sarmin, Atiya M.
El Moussaid, Nadia
Suntornnond, Ratima
Tyler, Eleanor J.
Kim, Yang-Hee
Di Cio, Stefania
Megone, William V.
Pearce, Oliver
Gautrot, Julien E.
Dawson, Jonathan
Connelly, John T.
author_sort Sarmin, Atiya M.
collection PubMed
description The extracellular matrix (ECM) is a complex mixture of structural proteins, proteoglycans, and signaling molecules that are essential for tissue integrity and homeostasis. While a number of recent studies have explored the use of decellularized ECM (dECM) as a biomaterial for tissue engineering, the complete composition, structure, and mechanics of these materials remain incompletely understood. In this study, we performed an in-depth characterization of skin-derived dECM biomaterials for human skin equivalent (HSE) models. The dECM materials were purified from porcine skin, and through mass spectrometry profiling, we quantified the presence of major ECM molecules, including types I, III, and VI collagen, fibrillin, and lumican. Rheological analysis demonstrated the sol-gel and shear-thinning properties of dECM materials, indicating their physical suitability as a tissue scaffold, while electron microscopy revealed a complex, hierarchical structure of nanofibers in dECM hydrogels. The dECM materials were compatible with advanced biofabrication techniques, including 3D printing within a gelatin microparticle support bath, printing with a sacrificial material, or blending with other ECM molecules to achieve more complex compositions and structures. As a proof of concept, we also demonstrate how dECM materials can be fabricated into a 3D skin wound healing model using 3D printing. Skin-derived dECM therefore represents a complex and versatile biomaterial with advantageous properties for the fabrication of next-generation HSEs.
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spelling pubmed-92214832022-06-24 Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models Sarmin, Atiya M. El Moussaid, Nadia Suntornnond, Ratima Tyler, Eleanor J. Kim, Yang-Hee Di Cio, Stefania Megone, William V. Pearce, Oliver Gautrot, Julien E. Dawson, Jonathan Connelly, John T. Biomolecules Article The extracellular matrix (ECM) is a complex mixture of structural proteins, proteoglycans, and signaling molecules that are essential for tissue integrity and homeostasis. While a number of recent studies have explored the use of decellularized ECM (dECM) as a biomaterial for tissue engineering, the complete composition, structure, and mechanics of these materials remain incompletely understood. In this study, we performed an in-depth characterization of skin-derived dECM biomaterials for human skin equivalent (HSE) models. The dECM materials were purified from porcine skin, and through mass spectrometry profiling, we quantified the presence of major ECM molecules, including types I, III, and VI collagen, fibrillin, and lumican. Rheological analysis demonstrated the sol-gel and shear-thinning properties of dECM materials, indicating their physical suitability as a tissue scaffold, while electron microscopy revealed a complex, hierarchical structure of nanofibers in dECM hydrogels. The dECM materials were compatible with advanced biofabrication techniques, including 3D printing within a gelatin microparticle support bath, printing with a sacrificial material, or blending with other ECM molecules to achieve more complex compositions and structures. As a proof of concept, we also demonstrate how dECM materials can be fabricated into a 3D skin wound healing model using 3D printing. Skin-derived dECM therefore represents a complex and versatile biomaterial with advantageous properties for the fabrication of next-generation HSEs. MDPI 2022-06-16 /pmc/articles/PMC9221483/ /pubmed/35740962 http://dx.doi.org/10.3390/biom12060837 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sarmin, Atiya M.
El Moussaid, Nadia
Suntornnond, Ratima
Tyler, Eleanor J.
Kim, Yang-Hee
Di Cio, Stefania
Megone, William V.
Pearce, Oliver
Gautrot, Julien E.
Dawson, Jonathan
Connelly, John T.
Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models
title Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models
title_full Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models
title_fullStr Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models
title_full_unstemmed Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models
title_short Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models
title_sort multi-scale analysis of the composition, structure, and function of decellularized extracellular matrix for human skin and wound healing models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221483/
https://www.ncbi.nlm.nih.gov/pubmed/35740962
http://dx.doi.org/10.3390/biom12060837
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