An Optimized Direct Lysis Gene Expression Microplate Assay and Applications for Disease, Differentiation, and Pharmacological Cell-Based Studies

Routine cell culture reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) gene expression analysis is limited in scalability due to minimum sample requirement and multistep isolation procedures. In this study, we aimed to optimize and apply a cost-effective and rapid protocol for d...

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Detalles Bibliográficos
Autores principales: Ng, Neville S., Maksour, Simon, Lum, Jeremy S., Newbery, Michelle, Shephard, Victoria, Ooi, Lezanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221485/
https://www.ncbi.nlm.nih.gov/pubmed/35735512
http://dx.doi.org/10.3390/bios12060364

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221485/
https://www.ncbi.nlm.nih.gov/pubmed/35735512
http://dx.doi.org/10.3390/bios12060364

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