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Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications

The development of resonance phenomena-based optical biosensors has gained relevance in recent years due to the excellent optical fiber properties and progress in the research on materials and techniques that allow resonance generation. However, for lossy mode resonance (LMR)-based sensors, the opti...

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Autores principales: Benítez, Melanys, Zubiate, Pablo, Del Villar, Ignacio, Socorro-Leránoz, Abián B., Matías, Ignacio R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221500/
https://www.ncbi.nlm.nih.gov/pubmed/35735551
http://dx.doi.org/10.3390/bios12060403
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author Benítez, Melanys
Zubiate, Pablo
Del Villar, Ignacio
Socorro-Leránoz, Abián B.
Matías, Ignacio R.
author_facet Benítez, Melanys
Zubiate, Pablo
Del Villar, Ignacio
Socorro-Leránoz, Abián B.
Matías, Ignacio R.
author_sort Benítez, Melanys
collection PubMed
description The development of resonance phenomena-based optical biosensors has gained relevance in recent years due to the excellent optical fiber properties and progress in the research on materials and techniques that allow resonance generation. However, for lossy mode resonance (LMR)-based sensors, the optical fiber presents disadvantages, such as the need for splicing the sensor head and the complex polarization control. To avoid these issues, planar waveguides such as coverslips are easier to handle, cost-effective, and more robust structures. In this work, a microfluidic LMR-based planar waveguide platform was proposed, and its use for biosensing applications was evaluated by detecting anti-immunoglobulin G (anti-IgG). In order to generate the wavelength resonance, the sensor surface was coated with a titanium dioxide (TiO(2)) thin-film. IgG antibodies were immobilized by covalent binding, and the detection assay was carried out by injecting anti-IgG in PBS buffer solutions from 5 to 20 μg/mL. The LMR wavelength shifted to higher values when increasing the analyte concentration, which means that the proposed system was able to detect the IgG/anti-IgG binding. The calibration curve was built from the experimental data obtained in three repetitions of the assay. In this way, a prototype of an LMR-based biosensing microfluidic platform developed on planar substrates was obtained for the first time.
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spelling pubmed-92215002022-06-24 Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications Benítez, Melanys Zubiate, Pablo Del Villar, Ignacio Socorro-Leránoz, Abián B. Matías, Ignacio R. Biosensors (Basel) Article The development of resonance phenomena-based optical biosensors has gained relevance in recent years due to the excellent optical fiber properties and progress in the research on materials and techniques that allow resonance generation. However, for lossy mode resonance (LMR)-based sensors, the optical fiber presents disadvantages, such as the need for splicing the sensor head and the complex polarization control. To avoid these issues, planar waveguides such as coverslips are easier to handle, cost-effective, and more robust structures. In this work, a microfluidic LMR-based planar waveguide platform was proposed, and its use for biosensing applications was evaluated by detecting anti-immunoglobulin G (anti-IgG). In order to generate the wavelength resonance, the sensor surface was coated with a titanium dioxide (TiO(2)) thin-film. IgG antibodies were immobilized by covalent binding, and the detection assay was carried out by injecting anti-IgG in PBS buffer solutions from 5 to 20 μg/mL. The LMR wavelength shifted to higher values when increasing the analyte concentration, which means that the proposed system was able to detect the IgG/anti-IgG binding. The calibration curve was built from the experimental data obtained in three repetitions of the assay. In this way, a prototype of an LMR-based biosensing microfluidic platform developed on planar substrates was obtained for the first time. MDPI 2022-06-10 /pmc/articles/PMC9221500/ /pubmed/35735551 http://dx.doi.org/10.3390/bios12060403 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Benítez, Melanys
Zubiate, Pablo
Del Villar, Ignacio
Socorro-Leránoz, Abián B.
Matías, Ignacio R.
Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications
title Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications
title_full Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications
title_fullStr Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications
title_full_unstemmed Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications
title_short Lossy Mode Resonance Based Microfluidic Platform Developed on Planar Waveguide for Biosensing Applications
title_sort lossy mode resonance based microfluidic platform developed on planar waveguide for biosensing applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221500/
https://www.ncbi.nlm.nih.gov/pubmed/35735551
http://dx.doi.org/10.3390/bios12060403
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