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Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response
Cholecystokinin 8 (CCK8) is an entero-octapeptide that participates in crosstalk with components of intestinal immunity via the CCK receptor (CCKR), but its role in modulation of the IgA response is not fully known under physiological conditions. Male eight-week-old BALB/c mice each were intraperito...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221551/ https://www.ncbi.nlm.nih.gov/pubmed/35735614 http://dx.doi.org/10.3390/cimb44060173 |
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author | Morales-Magaña, Juan Arciniega-Martínez, Ivonne Maciel Drago-Serrano, Maria Elisa Reséndiz-Albor, Aldo Arturo Jarillo-Luna, Rosa Adriana Cruz-Baquero, Andrea Gómez-López, Modesto Guzmán-Mejía, Fabiola Pacheco-Yépez, Judith |
author_facet | Morales-Magaña, Juan Arciniega-Martínez, Ivonne Maciel Drago-Serrano, Maria Elisa Reséndiz-Albor, Aldo Arturo Jarillo-Luna, Rosa Adriana Cruz-Baquero, Andrea Gómez-López, Modesto Guzmán-Mejía, Fabiola Pacheco-Yépez, Judith |
author_sort | Morales-Magaña, Juan |
collection | PubMed |
description | Cholecystokinin 8 (CCK8) is an entero-octapeptide that participates in crosstalk with components of intestinal immunity via the CCK receptor (CCKR), but its role in modulation of the IgA response is not fully known under physiological conditions. Male eight-week-old BALB/c mice each were intraperitoneally injected once during 7 days with CCK8, devazapide (CCKR1 antagonist), L365,260 (CCKR2 antagonist) or vehicle (sham group). In intestinal lavages, total and secretory IgA (SIgA) were determined by ELISA; in lamina propria, IgA(+) B lymphocytes and IgA(+) plasma cells were analyzed by flow cytometry; mRNA levels of polymeric immunoglobulin receptor (pIgR) in epithelial cells and α chain, interleukins (ILs) in lamina propria cells were assessed by qRTPCR. Regarding the sham conditions, IgA(+) plasma-cell percentage and IL-2, IL-5, IL-10 and transforming growth factor-β (TGF-β) mRNA levels were either increased by CCK8 or decreased by both CCKR antagonists. For IgA/SIgA responses, IL-4/IL-6 mRNA levels were decreased by all drugs and pIgR mRNA was increased by CCK8 and reduced by L365,260. IgA(+) B cell percentage and α chain mRNA levels were elicited by CCK8 and L365,260. Data suggested a presumable differential role of CCK/CCKR on the IgA-response; outcome of L365,260 on the elicitation of IgA(+) B cells and α chain mRNA needs further examination. |
format | Online Article Text |
id | pubmed-9221551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92215512022-06-24 Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response Morales-Magaña, Juan Arciniega-Martínez, Ivonne Maciel Drago-Serrano, Maria Elisa Reséndiz-Albor, Aldo Arturo Jarillo-Luna, Rosa Adriana Cruz-Baquero, Andrea Gómez-López, Modesto Guzmán-Mejía, Fabiola Pacheco-Yépez, Judith Curr Issues Mol Biol Brief Report Cholecystokinin 8 (CCK8) is an entero-octapeptide that participates in crosstalk with components of intestinal immunity via the CCK receptor (CCKR), but its role in modulation of the IgA response is not fully known under physiological conditions. Male eight-week-old BALB/c mice each were intraperitoneally injected once during 7 days with CCK8, devazapide (CCKR1 antagonist), L365,260 (CCKR2 antagonist) or vehicle (sham group). In intestinal lavages, total and secretory IgA (SIgA) were determined by ELISA; in lamina propria, IgA(+) B lymphocytes and IgA(+) plasma cells were analyzed by flow cytometry; mRNA levels of polymeric immunoglobulin receptor (pIgR) in epithelial cells and α chain, interleukins (ILs) in lamina propria cells were assessed by qRTPCR. Regarding the sham conditions, IgA(+) plasma-cell percentage and IL-2, IL-5, IL-10 and transforming growth factor-β (TGF-β) mRNA levels were either increased by CCK8 or decreased by both CCKR antagonists. For IgA/SIgA responses, IL-4/IL-6 mRNA levels were decreased by all drugs and pIgR mRNA was increased by CCK8 and reduced by L365,260. IgA(+) B cell percentage and α chain mRNA levels were elicited by CCK8 and L365,260. Data suggested a presumable differential role of CCK/CCKR on the IgA-response; outcome of L365,260 on the elicitation of IgA(+) B cells and α chain mRNA needs further examination. MDPI 2022-06-01 /pmc/articles/PMC9221551/ /pubmed/35735614 http://dx.doi.org/10.3390/cimb44060173 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Morales-Magaña, Juan Arciniega-Martínez, Ivonne Maciel Drago-Serrano, Maria Elisa Reséndiz-Albor, Aldo Arturo Jarillo-Luna, Rosa Adriana Cruz-Baquero, Andrea Gómez-López, Modesto Guzmán-Mejía, Fabiola Pacheco-Yépez, Judith Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response |
title | Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response |
title_full | Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response |
title_fullStr | Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response |
title_full_unstemmed | Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response |
title_short | Cholecystokinin Outcome on Markers of Intestinal IgA Antibody Response |
title_sort | cholecystokinin outcome on markers of intestinal iga antibody response |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221551/ https://www.ncbi.nlm.nih.gov/pubmed/35735614 http://dx.doi.org/10.3390/cimb44060173 |
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