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Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques

Centromeric satellite DNA (cen-satDNA) consists of highly divergent repeat monomers, each approximately 171 base pairs in length. Here, we investigated the genetic diversity in the centromeric region of two primate species: long-tailed (Macaca fascicularis) and rhesus (Macaca mulatta) macaques. Fluo...

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Autores principales: Singchat, Worapong, Ahmad, Syed Farhan, Jaisamut, Kitipong, Panthum, Thitipong, Ariyaraphong, Nattakan, Kraichak, Ekaphan, Muangmai, Narongrit, Duengkae, Prateep, Payungporn, Sunchai, Malaivijitnond, Suchinda, Srikulnath, Kornsorn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221937/
https://www.ncbi.nlm.nih.gov/pubmed/35741082
http://dx.doi.org/10.3390/cells11121953
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author Singchat, Worapong
Ahmad, Syed Farhan
Jaisamut, Kitipong
Panthum, Thitipong
Ariyaraphong, Nattakan
Kraichak, Ekaphan
Muangmai, Narongrit
Duengkae, Prateep
Payungporn, Sunchai
Malaivijitnond, Suchinda
Srikulnath, Kornsorn
author_facet Singchat, Worapong
Ahmad, Syed Farhan
Jaisamut, Kitipong
Panthum, Thitipong
Ariyaraphong, Nattakan
Kraichak, Ekaphan
Muangmai, Narongrit
Duengkae, Prateep
Payungporn, Sunchai
Malaivijitnond, Suchinda
Srikulnath, Kornsorn
author_sort Singchat, Worapong
collection PubMed
description Centromeric satellite DNA (cen-satDNA) consists of highly divergent repeat monomers, each approximately 171 base pairs in length. Here, we investigated the genetic diversity in the centromeric region of two primate species: long-tailed (Macaca fascicularis) and rhesus (Macaca mulatta) macaques. Fluorescence in situ hybridization and bioinformatic analysis showed the chromosome-specific organization and dynamic nature of cen-satDNAsequences, and their substantial diversity, with distinct subfamilies across macaque populations, suggesting increased turnovers. Comparative genomics identified high level polymorphisms spanning a 120 bp deletion region and a remarkable interspecific variability in cen-satDNA size and structure. Population structure analysis detected admixture patterns within populations, indicating their high divergence and rapid evolution. However, differences in cen-satDNA profiles appear to not be involved in hybrid incompatibility between the two species. Our study provides a genomic landscape of centromeric repeats in wild macaques and opens new avenues for exploring their impact on the adaptive evolution and speciation of primates.
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spelling pubmed-92219372022-06-24 Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques Singchat, Worapong Ahmad, Syed Farhan Jaisamut, Kitipong Panthum, Thitipong Ariyaraphong, Nattakan Kraichak, Ekaphan Muangmai, Narongrit Duengkae, Prateep Payungporn, Sunchai Malaivijitnond, Suchinda Srikulnath, Kornsorn Cells Article Centromeric satellite DNA (cen-satDNA) consists of highly divergent repeat monomers, each approximately 171 base pairs in length. Here, we investigated the genetic diversity in the centromeric region of two primate species: long-tailed (Macaca fascicularis) and rhesus (Macaca mulatta) macaques. Fluorescence in situ hybridization and bioinformatic analysis showed the chromosome-specific organization and dynamic nature of cen-satDNAsequences, and their substantial diversity, with distinct subfamilies across macaque populations, suggesting increased turnovers. Comparative genomics identified high level polymorphisms spanning a 120 bp deletion region and a remarkable interspecific variability in cen-satDNA size and structure. Population structure analysis detected admixture patterns within populations, indicating their high divergence and rapid evolution. However, differences in cen-satDNA profiles appear to not be involved in hybrid incompatibility between the two species. Our study provides a genomic landscape of centromeric repeats in wild macaques and opens new avenues for exploring their impact on the adaptive evolution and speciation of primates. MDPI 2022-06-17 /pmc/articles/PMC9221937/ /pubmed/35741082 http://dx.doi.org/10.3390/cells11121953 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Singchat, Worapong
Ahmad, Syed Farhan
Jaisamut, Kitipong
Panthum, Thitipong
Ariyaraphong, Nattakan
Kraichak, Ekaphan
Muangmai, Narongrit
Duengkae, Prateep
Payungporn, Sunchai
Malaivijitnond, Suchinda
Srikulnath, Kornsorn
Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques
title Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques
title_full Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques
title_fullStr Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques
title_full_unstemmed Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques
title_short Population Scale Analysis of Centromeric Satellite DNA Reveals Highly Dynamic Evolutionary Patterns and Genomic Organization in Long-Tailed and Rhesus Macaques
title_sort population scale analysis of centromeric satellite dna reveals highly dynamic evolutionary patterns and genomic organization in long-tailed and rhesus macaques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221937/
https://www.ncbi.nlm.nih.gov/pubmed/35741082
http://dx.doi.org/10.3390/cells11121953
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