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Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update

The emergence of the COVID-19 pandemic prompted fast development of novel diagnostic methods of the etiologic virus SARS-CoV-2. Methods based on CRISPR–Cas systems have been particularly promising because they can achieve a similar sensitivity and specificity to the benchmark RT-qPCR, especially whe...

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Autores principales: Hernandez-Garcia, Armando, Morales-Moreno, Melissa D., Valdés-Galindo, Erick G., Jimenez-Nieto, Eric P., Quezada, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222122/
https://www.ncbi.nlm.nih.gov/pubmed/35741243
http://dx.doi.org/10.3390/diagnostics12061434
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author Hernandez-Garcia, Armando
Morales-Moreno, Melissa D.
Valdés-Galindo, Erick G.
Jimenez-Nieto, Eric P.
Quezada, Andrea
author_facet Hernandez-Garcia, Armando
Morales-Moreno, Melissa D.
Valdés-Galindo, Erick G.
Jimenez-Nieto, Eric P.
Quezada, Andrea
author_sort Hernandez-Garcia, Armando
collection PubMed
description The emergence of the COVID-19 pandemic prompted fast development of novel diagnostic methods of the etiologic virus SARS-CoV-2. Methods based on CRISPR–Cas systems have been particularly promising because they can achieve a similar sensitivity and specificity to the benchmark RT-qPCR, especially when coupled to an isothermal pre-amplification step. Furthermore, they have also solved inherent limitations of RT-qPCR that impede its decentralized use and deployment in the field, such as the need for expensive equipment, high cost per reaction, and delivery of results in hours, among others. In this review, we evaluate publicly available methods to detect SARS-CoV-2 that are based on CRISPR–Cas and isothermal amplification. We critically analyze the steps required to obtain a successful result from clinical samples and pinpoint key experimental conditions and parameters that could be optimized or modified to improve clinical and analytical outputs. The COVID outbreak has propelled intensive research in a short time, which is paving the way to develop effective and very promising CRISPR–Cas systems for the precise detection of SARS-CoV-2. This review could also serve as an introductory guide to new labs delving into this technology.
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spelling pubmed-92221222022-06-24 Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update Hernandez-Garcia, Armando Morales-Moreno, Melissa D. Valdés-Galindo, Erick G. Jimenez-Nieto, Eric P. Quezada, Andrea Diagnostics (Basel) Review The emergence of the COVID-19 pandemic prompted fast development of novel diagnostic methods of the etiologic virus SARS-CoV-2. Methods based on CRISPR–Cas systems have been particularly promising because they can achieve a similar sensitivity and specificity to the benchmark RT-qPCR, especially when coupled to an isothermal pre-amplification step. Furthermore, they have also solved inherent limitations of RT-qPCR that impede its decentralized use and deployment in the field, such as the need for expensive equipment, high cost per reaction, and delivery of results in hours, among others. In this review, we evaluate publicly available methods to detect SARS-CoV-2 that are based on CRISPR–Cas and isothermal amplification. We critically analyze the steps required to obtain a successful result from clinical samples and pinpoint key experimental conditions and parameters that could be optimized or modified to improve clinical and analytical outputs. The COVID outbreak has propelled intensive research in a short time, which is paving the way to develop effective and very promising CRISPR–Cas systems for the precise detection of SARS-CoV-2. This review could also serve as an introductory guide to new labs delving into this technology. MDPI 2022-06-10 /pmc/articles/PMC9222122/ /pubmed/35741243 http://dx.doi.org/10.3390/diagnostics12061434 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Hernandez-Garcia, Armando
Morales-Moreno, Melissa D.
Valdés-Galindo, Erick G.
Jimenez-Nieto, Eric P.
Quezada, Andrea
Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update
title Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update
title_full Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update
title_fullStr Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update
title_full_unstemmed Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update
title_short Diagnostics of COVID-19 Based on CRISPR–Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update
title_sort diagnostics of covid-19 based on crispr–cas coupled to isothermal amplification: a comparative analysis and update
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222122/
https://www.ncbi.nlm.nih.gov/pubmed/35741243
http://dx.doi.org/10.3390/diagnostics12061434
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