Cargando…

Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks

Fiber length is an important indicator of cotton fiber quality, and the time and rate of cotton fiber cell elongation are key factors in determining the fiber length of mature cotton. To gain insight into the differences in fiber elongation mechanisms in the offspring of backcross populations of Sea...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Shengmei, Geng, Shiwei, Pang, Bo, Zhao, Jieyin, Huang, Yajie, Rui, Cun, Cui, Jinxin, Jiao, Yang, Zhang, Ru, Gao, Wenwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222338/
https://www.ncbi.nlm.nih.gov/pubmed/35741716
http://dx.doi.org/10.3390/genes13060954
_version_ 1784732849713184768
author Li, Shengmei
Geng, Shiwei
Pang, Bo
Zhao, Jieyin
Huang, Yajie
Rui, Cun
Cui, Jinxin
Jiao, Yang
Zhang, Ru
Gao, Wenwei
author_facet Li, Shengmei
Geng, Shiwei
Pang, Bo
Zhao, Jieyin
Huang, Yajie
Rui, Cun
Cui, Jinxin
Jiao, Yang
Zhang, Ru
Gao, Wenwei
author_sort Li, Shengmei
collection PubMed
description Fiber length is an important indicator of cotton fiber quality, and the time and rate of cotton fiber cell elongation are key factors in determining the fiber length of mature cotton. To gain insight into the differences in fiber elongation mechanisms in the offspring of backcross populations of Sea Island cotton Xinhai 16 and land cotton Line 9, we selected two groups with significant differences in fiber length (long-fiber group L and short-fiber group S) at different fiber development stages 0, 5, 10 and 15 days post-anthesis (DPA) for transcriptome comparison. A total of 171.74 Gb of clean data was obtained by RNA-seq, and eight genes were randomly selected for qPCR validation. Data analysis identified 6055 differentially expressed genes (DEGs) between two groups of fibers, L and S, in four developmental periods, and gene ontology (GO) term analysis revealed that these DEGs were associated mainly with microtubule driving, reactive oxygen species, plant cell wall biosynthesis, and glycosyl compound hydrolase activity. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that plant hormone signaling, mitogen-activated protein kinase (MAPK) signaling, and starch and sucrose metabolism pathways were associated with fiber elongation. Subsequently, a sustained upregulation expression pattern, profile 19, was identified and analyzed using short time-series expression miner (STEM). An analysis of the weighted gene coexpression network module uncovered 21 genes closely related to fiber development, mainly involved in functions such as cell wall relaxation, microtubule formation, and cytoskeletal structure of the cell wall. This study helps to enhance the understanding of the Sea Island–Upland backcross population and identifies key genes for cotton fiber development, and these findings will provide a basis for future research on the molecular mechanisms of fiber length formation in cotton populations.
format Online
Article
Text
id pubmed-9222338
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92223382022-06-24 Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks Li, Shengmei Geng, Shiwei Pang, Bo Zhao, Jieyin Huang, Yajie Rui, Cun Cui, Jinxin Jiao, Yang Zhang, Ru Gao, Wenwei Genes (Basel) Article Fiber length is an important indicator of cotton fiber quality, and the time and rate of cotton fiber cell elongation are key factors in determining the fiber length of mature cotton. To gain insight into the differences in fiber elongation mechanisms in the offspring of backcross populations of Sea Island cotton Xinhai 16 and land cotton Line 9, we selected two groups with significant differences in fiber length (long-fiber group L and short-fiber group S) at different fiber development stages 0, 5, 10 and 15 days post-anthesis (DPA) for transcriptome comparison. A total of 171.74 Gb of clean data was obtained by RNA-seq, and eight genes were randomly selected for qPCR validation. Data analysis identified 6055 differentially expressed genes (DEGs) between two groups of fibers, L and S, in four developmental periods, and gene ontology (GO) term analysis revealed that these DEGs were associated mainly with microtubule driving, reactive oxygen species, plant cell wall biosynthesis, and glycosyl compound hydrolase activity. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that plant hormone signaling, mitogen-activated protein kinase (MAPK) signaling, and starch and sucrose metabolism pathways were associated with fiber elongation. Subsequently, a sustained upregulation expression pattern, profile 19, was identified and analyzed using short time-series expression miner (STEM). An analysis of the weighted gene coexpression network module uncovered 21 genes closely related to fiber development, mainly involved in functions such as cell wall relaxation, microtubule formation, and cytoskeletal structure of the cell wall. This study helps to enhance the understanding of the Sea Island–Upland backcross population and identifies key genes for cotton fiber development, and these findings will provide a basis for future research on the molecular mechanisms of fiber length formation in cotton populations. MDPI 2022-05-26 /pmc/articles/PMC9222338/ /pubmed/35741716 http://dx.doi.org/10.3390/genes13060954 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Shengmei
Geng, Shiwei
Pang, Bo
Zhao, Jieyin
Huang, Yajie
Rui, Cun
Cui, Jinxin
Jiao, Yang
Zhang, Ru
Gao, Wenwei
Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks
title Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks
title_full Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks
title_fullStr Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks
title_full_unstemmed Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks
title_short Revealing Genetic Differences in Fiber Elongation between the Offspring of Sea Island Cotton and Upland Cotton Backcross Populations Based on Transcriptome and Weighted Gene Coexpression Networks
title_sort revealing genetic differences in fiber elongation between the offspring of sea island cotton and upland cotton backcross populations based on transcriptome and weighted gene coexpression networks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222338/
https://www.ncbi.nlm.nih.gov/pubmed/35741716
http://dx.doi.org/10.3390/genes13060954
work_keys_str_mv AT lishengmei revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT gengshiwei revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT pangbo revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT zhaojieyin revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT huangyajie revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT ruicun revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT cuijinxin revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT jiaoyang revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT zhangru revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks
AT gaowenwei revealinggeneticdifferencesinfiberelongationbetweentheoffspringofseaislandcottonanduplandcottonbackcrosspopulationsbasedontranscriptomeandweightedgenecoexpressionnetworks