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Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks

Three-dimensional (3D) printing has great potential for creating tissues and organs to meet shortfalls in transplant supply, and biomaterial inks are key components of many such approaches. There is a need for biomaterial inks that facilitate integration, infiltration, and vascularization of targete...

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Detalles Bibliográficos
Autores principales: Panarello, Andrew Philip, Seavey, Corey Edward, Doshi, Mona, Dickerson, Andrew K., Kean, Thomas J., Willenberg, Bradley Jay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222415/
https://www.ncbi.nlm.nih.gov/pubmed/35735720
http://dx.doi.org/10.3390/gels8060376
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author Panarello, Andrew Philip
Seavey, Corey Edward
Doshi, Mona
Dickerson, Andrew K.
Kean, Thomas J.
Willenberg, Bradley Jay
author_facet Panarello, Andrew Philip
Seavey, Corey Edward
Doshi, Mona
Dickerson, Andrew K.
Kean, Thomas J.
Willenberg, Bradley Jay
author_sort Panarello, Andrew Philip
collection PubMed
description Three-dimensional (3D) printing has great potential for creating tissues and organs to meet shortfalls in transplant supply, and biomaterial inks are key components of many such approaches. There is a need for biomaterial inks that facilitate integration, infiltration, and vascularization of targeted 3D-printed structures. This study is therefore focused on creating new biomaterial inks from self-assembled capillary alginate gel (Capgel), which possesses a unique microstructure of uniform tubular channels with tunable diameters and densities. First, extrusions of Capgel through needles (0.1–0.8 mm inner diameter) were investigated. It was found that Capgel ink extrudes as slurries of fractured and entangled particles, each retaining capillary microstructures, and that extruded line widths W and particle sizes A were both functions of needle inner diameter D, specifically power-law relationships of W~D(0.42) and A~D(1.52), respectively. Next, various structures were successfully 3D-printed with Capgel ink, thus demonstrating that this biomaterial ink is stackable and self-supporting. To increase ink self-adherence, Capgel was coated with poly-L-lysine (PLL) to create a cationic “skin” prior to extrusion. It was hypothesized that, during extrusion of Capgel-PLL, the sheared particles fracture and thereby expose cryptic sites of negatively-charged biomaterial capable of forming new polyelectrolyte bonds with areas of the positively-charged PLL skin on neighboring entangled particles. This novel approach resulted in continuous, self-adherent extrusions that remained intact in solution. Human lung fibroblasts (HLFs) were then cultured on this ink to investigate biocompatibility. HLFs readily colonized Capgel-PLL ink and were strongly oriented by the capillary microstructures. This is the first description of successful 3D-printing with Capgel biomaterial ink as well as the first demonstration of the concept and formulation of a self-adherent Capgel-PLL biomaterial ink.
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spelling pubmed-92224152022-06-24 Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks Panarello, Andrew Philip Seavey, Corey Edward Doshi, Mona Dickerson, Andrew K. Kean, Thomas J. Willenberg, Bradley Jay Gels Article Three-dimensional (3D) printing has great potential for creating tissues and organs to meet shortfalls in transplant supply, and biomaterial inks are key components of many such approaches. There is a need for biomaterial inks that facilitate integration, infiltration, and vascularization of targeted 3D-printed structures. This study is therefore focused on creating new biomaterial inks from self-assembled capillary alginate gel (Capgel), which possesses a unique microstructure of uniform tubular channels with tunable diameters and densities. First, extrusions of Capgel through needles (0.1–0.8 mm inner diameter) were investigated. It was found that Capgel ink extrudes as slurries of fractured and entangled particles, each retaining capillary microstructures, and that extruded line widths W and particle sizes A were both functions of needle inner diameter D, specifically power-law relationships of W~D(0.42) and A~D(1.52), respectively. Next, various structures were successfully 3D-printed with Capgel ink, thus demonstrating that this biomaterial ink is stackable and self-supporting. To increase ink self-adherence, Capgel was coated with poly-L-lysine (PLL) to create a cationic “skin” prior to extrusion. It was hypothesized that, during extrusion of Capgel-PLL, the sheared particles fracture and thereby expose cryptic sites of negatively-charged biomaterial capable of forming new polyelectrolyte bonds with areas of the positively-charged PLL skin on neighboring entangled particles. This novel approach resulted in continuous, self-adherent extrusions that remained intact in solution. Human lung fibroblasts (HLFs) were then cultured on this ink to investigate biocompatibility. HLFs readily colonized Capgel-PLL ink and were strongly oriented by the capillary microstructures. This is the first description of successful 3D-printing with Capgel biomaterial ink as well as the first demonstration of the concept and formulation of a self-adherent Capgel-PLL biomaterial ink. MDPI 2022-06-14 /pmc/articles/PMC9222415/ /pubmed/35735720 http://dx.doi.org/10.3390/gels8060376 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panarello, Andrew Philip
Seavey, Corey Edward
Doshi, Mona
Dickerson, Andrew K.
Kean, Thomas J.
Willenberg, Bradley Jay
Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks
title Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks
title_full Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks
title_fullStr Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks
title_full_unstemmed Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks
title_short Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks
title_sort transforming capillary alginate gel (capgel) into new 3d-printing biomaterial inks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222415/
https://www.ncbi.nlm.nih.gov/pubmed/35735720
http://dx.doi.org/10.3390/gels8060376
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