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Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases

The common carp is a hypoxia-tolerant fish, and the understanding of its ability to live in low-oxygen environments has been applied to human health issues such as cancer and neuron degeneration. Here, we investigated differential gene expression changes during hypoxia in five common carp organs inc...

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Autores principales: Hung, Kuo-Sheng, Chen, Shiow-Yi, Hsu, Pang-Hung, Lin, Bo-An, Hu, Chin-Hua, Yang, Cing-Han, Pai, Tun-Wen, Tzou, Wen-Shyong, Chung, Hsin-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222487/
https://www.ncbi.nlm.nih.gov/pubmed/35741857
http://dx.doi.org/10.3390/genes13061096
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author Hung, Kuo-Sheng
Chen, Shiow-Yi
Hsu, Pang-Hung
Lin, Bo-An
Hu, Chin-Hua
Yang, Cing-Han
Pai, Tun-Wen
Tzou, Wen-Shyong
Chung, Hsin-Yu
author_facet Hung, Kuo-Sheng
Chen, Shiow-Yi
Hsu, Pang-Hung
Lin, Bo-An
Hu, Chin-Hua
Yang, Cing-Han
Pai, Tun-Wen
Tzou, Wen-Shyong
Chung, Hsin-Yu
author_sort Hung, Kuo-Sheng
collection PubMed
description The common carp is a hypoxia-tolerant fish, and the understanding of its ability to live in low-oxygen environments has been applied to human health issues such as cancer and neuron degeneration. Here, we investigated differential gene expression changes during hypoxia in five common carp organs including the brain, the gill, the head kidney, the liver, and the intestine. Based on RNA sequencing, gene expression changes under hypoxic conditions were detected in over 1800 genes in common carp. The analysis of these genes further revealed that all five organs had high expression-specific properties. According to the results of the GO and KEGG, the pathways involved in the adaptation to hypoxia provided information on responses specific to each organ in low oxygen, such as glucose metabolism and energy usage, cholesterol synthesis, cell cycle, circadian rhythm, and dopamine activation. DisGeNET analysis showed that some human diseases such as cancer, diabetes, epilepsy, metabolism diseases, and social ability disorders were related to hypoxia-regulated genes. Our results suggested that common carp undergo various gene regulations in different organs under hypoxic conditions, and integrative bioinformatics may provide some potential targets for advancing disease research.
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spelling pubmed-92224872022-06-24 Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases Hung, Kuo-Sheng Chen, Shiow-Yi Hsu, Pang-Hung Lin, Bo-An Hu, Chin-Hua Yang, Cing-Han Pai, Tun-Wen Tzou, Wen-Shyong Chung, Hsin-Yu Genes (Basel) Article The common carp is a hypoxia-tolerant fish, and the understanding of its ability to live in low-oxygen environments has been applied to human health issues such as cancer and neuron degeneration. Here, we investigated differential gene expression changes during hypoxia in five common carp organs including the brain, the gill, the head kidney, the liver, and the intestine. Based on RNA sequencing, gene expression changes under hypoxic conditions were detected in over 1800 genes in common carp. The analysis of these genes further revealed that all five organs had high expression-specific properties. According to the results of the GO and KEGG, the pathways involved in the adaptation to hypoxia provided information on responses specific to each organ in low oxygen, such as glucose metabolism and energy usage, cholesterol synthesis, cell cycle, circadian rhythm, and dopamine activation. DisGeNET analysis showed that some human diseases such as cancer, diabetes, epilepsy, metabolism diseases, and social ability disorders were related to hypoxia-regulated genes. Our results suggested that common carp undergo various gene regulations in different organs under hypoxic conditions, and integrative bioinformatics may provide some potential targets for advancing disease research. MDPI 2022-06-19 /pmc/articles/PMC9222487/ /pubmed/35741857 http://dx.doi.org/10.3390/genes13061096 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hung, Kuo-Sheng
Chen, Shiow-Yi
Hsu, Pang-Hung
Lin, Bo-An
Hu, Chin-Hua
Yang, Cing-Han
Pai, Tun-Wen
Tzou, Wen-Shyong
Chung, Hsin-Yu
Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases
title Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases
title_full Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases
title_fullStr Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases
title_full_unstemmed Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases
title_short Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases
title_sort comparative transcriptome analysis of organ-specific adaptive responses to hypoxia provides insights to human diseases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222487/
https://www.ncbi.nlm.nih.gov/pubmed/35741857
http://dx.doi.org/10.3390/genes13061096
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