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Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing

Drug administration to the wound site is a potential method for wound healing. The drug retention duration should be extended, and drug permeability through the buccal mucosal layer should be regulated. Oral wounds can be caused by inflammation, ulcers, trauma, or pathological lesions; if these woun...

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Autores principales: Hosny, Khaled M., Naveen, N. Raghavendra, Kurakula, Mallesh, Sindi, Amal M., Sabei, Fahad Y., Fatease, Adel Al, Jali, Abdulmajeed M., Alharbi, Waleed S., Mushtaq, Rayan Y., Felemban, Majed, Tayeb, Hossam H., Alfayez, Eman, Rizg, Waleed Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222678/
https://www.ncbi.nlm.nih.gov/pubmed/35735729
http://dx.doi.org/10.3390/gels8060385
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author Hosny, Khaled M.
Naveen, N. Raghavendra
Kurakula, Mallesh
Sindi, Amal M.
Sabei, Fahad Y.
Fatease, Adel Al
Jali, Abdulmajeed M.
Alharbi, Waleed S.
Mushtaq, Rayan Y.
Felemban, Majed
Tayeb, Hossam H.
Alfayez, Eman
Rizg, Waleed Y.
author_facet Hosny, Khaled M.
Naveen, N. Raghavendra
Kurakula, Mallesh
Sindi, Amal M.
Sabei, Fahad Y.
Fatease, Adel Al
Jali, Abdulmajeed M.
Alharbi, Waleed S.
Mushtaq, Rayan Y.
Felemban, Majed
Tayeb, Hossam H.
Alfayez, Eman
Rizg, Waleed Y.
author_sort Hosny, Khaled M.
collection PubMed
description Drug administration to the wound site is a potential method for wound healing. The drug retention duration should be extended, and drug permeability through the buccal mucosal layer should be regulated. Oral wounds can be caused by inflammation, ulcers, trauma, or pathological lesions; if these wounds are not treated properly, they can lead to pain, infection, and subsequent undesirable scarring. This study aimed to develop Kolliphor-407 P-based gel containing neomycin sulfate (NES) loaded in solid lipid nanoparticles (SLNs) and enhance the antimicrobial activity. By considering lipid concentrations and achieving the lowest particle size (Y1) and maximum entrapment (EE-Y2) effectiveness, the formulation of NES-SLN was optimized using the Box–Behnken design. For the selected responses, 17 runs were formulated (as anticipated by the Design-Expert software) and evaluated accordingly. The optimized formulation could achieve a particle size of 196.25 and EE of 89.27% and was further utilized to prepare the gel formulation. The NES-SLN-G formula was discovered to have a smooth, homogeneous structure and good mechanical and rheological properties. After 24 h of treatment, NES-SLN-G showed a regulated in vitro drug release pattern, excellent ex vivo permeability, and increased in vitro antibacterial activity. These findings indicate the potential application of NES-SLN-loaded gels as a promising formulation for buccal mucosal wound healing.
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spelling pubmed-92226782022-06-24 Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing Hosny, Khaled M. Naveen, N. Raghavendra Kurakula, Mallesh Sindi, Amal M. Sabei, Fahad Y. Fatease, Adel Al Jali, Abdulmajeed M. Alharbi, Waleed S. Mushtaq, Rayan Y. Felemban, Majed Tayeb, Hossam H. Alfayez, Eman Rizg, Waleed Y. Gels Article Drug administration to the wound site is a potential method for wound healing. The drug retention duration should be extended, and drug permeability through the buccal mucosal layer should be regulated. Oral wounds can be caused by inflammation, ulcers, trauma, or pathological lesions; if these wounds are not treated properly, they can lead to pain, infection, and subsequent undesirable scarring. This study aimed to develop Kolliphor-407 P-based gel containing neomycin sulfate (NES) loaded in solid lipid nanoparticles (SLNs) and enhance the antimicrobial activity. By considering lipid concentrations and achieving the lowest particle size (Y1) and maximum entrapment (EE-Y2) effectiveness, the formulation of NES-SLN was optimized using the Box–Behnken design. For the selected responses, 17 runs were formulated (as anticipated by the Design-Expert software) and evaluated accordingly. The optimized formulation could achieve a particle size of 196.25 and EE of 89.27% and was further utilized to prepare the gel formulation. The NES-SLN-G formula was discovered to have a smooth, homogeneous structure and good mechanical and rheological properties. After 24 h of treatment, NES-SLN-G showed a regulated in vitro drug release pattern, excellent ex vivo permeability, and increased in vitro antibacterial activity. These findings indicate the potential application of NES-SLN-loaded gels as a promising formulation for buccal mucosal wound healing. MDPI 2022-06-16 /pmc/articles/PMC9222678/ /pubmed/35735729 http://dx.doi.org/10.3390/gels8060385 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hosny, Khaled M.
Naveen, N. Raghavendra
Kurakula, Mallesh
Sindi, Amal M.
Sabei, Fahad Y.
Fatease, Adel Al
Jali, Abdulmajeed M.
Alharbi, Waleed S.
Mushtaq, Rayan Y.
Felemban, Majed
Tayeb, Hossam H.
Alfayez, Eman
Rizg, Waleed Y.
Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing
title Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing
title_full Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing
title_fullStr Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing
title_full_unstemmed Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing
title_short Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing
title_sort design and development of neomycin sulfate gel loaded with solid lipid nanoparticles for buccal mucosal wound healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222678/
https://www.ncbi.nlm.nih.gov/pubmed/35735729
http://dx.doi.org/10.3390/gels8060385
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