Cargando…

Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation

Biomaterials have been investigated as an alternative for the treatment of bone defects, such as chitosan/carbon nanotubes scaffolds, which allow cell proliferation. However, bone regeneration can be accelerated by electrotherapeutic resources that act on bone metabolism, such as low-level laser the...

Descripción completa

Detalles Bibliográficos
Autores principales: Silva, Samantha Ketelyn, Plepis, Ana Maria Guzzi, Martins, Virginia da Conceição Amaro, Horn, Marilia Marta, Buchaim, Daniela Vieira, Buchaim, Rogerio Leone, Pelegrine, André Antônio, Silva, Vinícius Rodrigues, Kudo, Mateus Hissashi Matsumoto, Fernandes, José Francisco Rebello, Nazari, Fabricio Montenegro, da Cunha, Marcelo Rodrigues
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223695/
https://www.ncbi.nlm.nih.gov/pubmed/35742948
http://dx.doi.org/10.3390/ijms23126503
_version_ 1784733187884187648
author Silva, Samantha Ketelyn
Plepis, Ana Maria Guzzi
Martins, Virginia da Conceição Amaro
Horn, Marilia Marta
Buchaim, Daniela Vieira
Buchaim, Rogerio Leone
Pelegrine, André Antônio
Silva, Vinícius Rodrigues
Kudo, Mateus Hissashi Matsumoto
Fernandes, José Francisco Rebello
Nazari, Fabricio Montenegro
da Cunha, Marcelo Rodrigues
author_facet Silva, Samantha Ketelyn
Plepis, Ana Maria Guzzi
Martins, Virginia da Conceição Amaro
Horn, Marilia Marta
Buchaim, Daniela Vieira
Buchaim, Rogerio Leone
Pelegrine, André Antônio
Silva, Vinícius Rodrigues
Kudo, Mateus Hissashi Matsumoto
Fernandes, José Francisco Rebello
Nazari, Fabricio Montenegro
da Cunha, Marcelo Rodrigues
author_sort Silva, Samantha Ketelyn
collection PubMed
description Biomaterials have been investigated as an alternative for the treatment of bone defects, such as chitosan/carbon nanotubes scaffolds, which allow cell proliferation. However, bone regeneration can be accelerated by electrotherapeutic resources that act on bone metabolism, such as low-level laser therapy (LLLT). Thus, this study evaluated the regeneration of bone lesions grafted with chitosan/carbon nanotubes scaffolds and associated with LLLT. For this, a defect (3 mm) was created in the femur of thirty rats, which were divided into 6 groups: Control (G1/Control), LLLT (G2/Laser), Chitosan/Carbon Nanotubes (G3/C+CNTs), Chitosan/Carbon Nanotubes with LLLT (G4/C+CNTs+L), Mineralized Chitosan/Carbon Nanotubes (G5/C+CNTsM) and Mineralized Chitosan/Carbon Nanotubes with LLLT (G6/C+CNTsM+L). After 5 weeks, the biocompatibility of the chitosan/carbon nanotubes scaffolds was observed, with the absence of inflammatory infiltrates and fibrotic tissue. Bone neoformation was denser, thicker and voluminous in G6/C+CNTsM+L. Histomorphometric analyses showed that the relative percentage and standard deviations (mean ± SD) of new bone formation in groups G1 to G6 were 59.93 ± 3.04a (G1/Control), 70.83 ± 1.21b (G2/Laser), 70.09 ± 4.31b (G3/C+CNTs), 81.6 ± 5.74c (G4/C+CNTs+L), 81.4 ± 4.57c (G5/C+CNTsM) and 91.3 ± 4.81d (G6/C+CNTsM+L), respectively, with G6 showing a significant difference in relation to the other groups (a ≠ b ≠ c ≠ d; p < 0.05). Immunohistochemistry also revealed good expression of osteocalcin (OC), osteopontin (OP) and vascular endothelial growth factor (VEGF). It was concluded that chitosan-based carbon nanotube materials combined with LLLT effectively stimulated the bone healing process.
format Online
Article
Text
id pubmed-9223695
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92236952022-06-24 Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation Silva, Samantha Ketelyn Plepis, Ana Maria Guzzi Martins, Virginia da Conceição Amaro Horn, Marilia Marta Buchaim, Daniela Vieira Buchaim, Rogerio Leone Pelegrine, André Antônio Silva, Vinícius Rodrigues Kudo, Mateus Hissashi Matsumoto Fernandes, José Francisco Rebello Nazari, Fabricio Montenegro da Cunha, Marcelo Rodrigues Int J Mol Sci Article Biomaterials have been investigated as an alternative for the treatment of bone defects, such as chitosan/carbon nanotubes scaffolds, which allow cell proliferation. However, bone regeneration can be accelerated by electrotherapeutic resources that act on bone metabolism, such as low-level laser therapy (LLLT). Thus, this study evaluated the regeneration of bone lesions grafted with chitosan/carbon nanotubes scaffolds and associated with LLLT. For this, a defect (3 mm) was created in the femur of thirty rats, which were divided into 6 groups: Control (G1/Control), LLLT (G2/Laser), Chitosan/Carbon Nanotubes (G3/C+CNTs), Chitosan/Carbon Nanotubes with LLLT (G4/C+CNTs+L), Mineralized Chitosan/Carbon Nanotubes (G5/C+CNTsM) and Mineralized Chitosan/Carbon Nanotubes with LLLT (G6/C+CNTsM+L). After 5 weeks, the biocompatibility of the chitosan/carbon nanotubes scaffolds was observed, with the absence of inflammatory infiltrates and fibrotic tissue. Bone neoformation was denser, thicker and voluminous in G6/C+CNTsM+L. Histomorphometric analyses showed that the relative percentage and standard deviations (mean ± SD) of new bone formation in groups G1 to G6 were 59.93 ± 3.04a (G1/Control), 70.83 ± 1.21b (G2/Laser), 70.09 ± 4.31b (G3/C+CNTs), 81.6 ± 5.74c (G4/C+CNTs+L), 81.4 ± 4.57c (G5/C+CNTsM) and 91.3 ± 4.81d (G6/C+CNTsM+L), respectively, with G6 showing a significant difference in relation to the other groups (a ≠ b ≠ c ≠ d; p < 0.05). Immunohistochemistry also revealed good expression of osteocalcin (OC), osteopontin (OP) and vascular endothelial growth factor (VEGF). It was concluded that chitosan-based carbon nanotube materials combined with LLLT effectively stimulated the bone healing process. MDPI 2022-06-10 /pmc/articles/PMC9223695/ /pubmed/35742948 http://dx.doi.org/10.3390/ijms23126503 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Samantha Ketelyn
Plepis, Ana Maria Guzzi
Martins, Virginia da Conceição Amaro
Horn, Marilia Marta
Buchaim, Daniela Vieira
Buchaim, Rogerio Leone
Pelegrine, André Antônio
Silva, Vinícius Rodrigues
Kudo, Mateus Hissashi Matsumoto
Fernandes, José Francisco Rebello
Nazari, Fabricio Montenegro
da Cunha, Marcelo Rodrigues
Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation
title Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation
title_full Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation
title_fullStr Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation
title_full_unstemmed Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation
title_short Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation
title_sort suitability of chitosan scaffolds with carbon nanotubes for bone defects treated with photobiomodulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223695/
https://www.ncbi.nlm.nih.gov/pubmed/35742948
http://dx.doi.org/10.3390/ijms23126503
work_keys_str_mv AT silvasamanthaketelyn suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT plepisanamariaguzzi suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT martinsvirginiadaconceicaoamaro suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT hornmariliamarta suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT buchaimdanielavieira suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT buchaimrogerioleone suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT pelegrineandreantonio suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT silvaviniciusrodrigues suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT kudomateushissashimatsumoto suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT fernandesjosefranciscorebello suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT nazarifabriciomontenegro suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation
AT dacunhamarcelorodrigues suitabilityofchitosanscaffoldswithcarbonnanotubesforbonedefectstreatedwithphotobiomodulation