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Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation
Biomaterials have been investigated as an alternative for the treatment of bone defects, such as chitosan/carbon nanotubes scaffolds, which allow cell proliferation. However, bone regeneration can be accelerated by electrotherapeutic resources that act on bone metabolism, such as low-level laser the...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223695/ https://www.ncbi.nlm.nih.gov/pubmed/35742948 http://dx.doi.org/10.3390/ijms23126503 |
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author | Silva, Samantha Ketelyn Plepis, Ana Maria Guzzi Martins, Virginia da Conceição Amaro Horn, Marilia Marta Buchaim, Daniela Vieira Buchaim, Rogerio Leone Pelegrine, André Antônio Silva, Vinícius Rodrigues Kudo, Mateus Hissashi Matsumoto Fernandes, José Francisco Rebello Nazari, Fabricio Montenegro da Cunha, Marcelo Rodrigues |
author_facet | Silva, Samantha Ketelyn Plepis, Ana Maria Guzzi Martins, Virginia da Conceição Amaro Horn, Marilia Marta Buchaim, Daniela Vieira Buchaim, Rogerio Leone Pelegrine, André Antônio Silva, Vinícius Rodrigues Kudo, Mateus Hissashi Matsumoto Fernandes, José Francisco Rebello Nazari, Fabricio Montenegro da Cunha, Marcelo Rodrigues |
author_sort | Silva, Samantha Ketelyn |
collection | PubMed |
description | Biomaterials have been investigated as an alternative for the treatment of bone defects, such as chitosan/carbon nanotubes scaffolds, which allow cell proliferation. However, bone regeneration can be accelerated by electrotherapeutic resources that act on bone metabolism, such as low-level laser therapy (LLLT). Thus, this study evaluated the regeneration of bone lesions grafted with chitosan/carbon nanotubes scaffolds and associated with LLLT. For this, a defect (3 mm) was created in the femur of thirty rats, which were divided into 6 groups: Control (G1/Control), LLLT (G2/Laser), Chitosan/Carbon Nanotubes (G3/C+CNTs), Chitosan/Carbon Nanotubes with LLLT (G4/C+CNTs+L), Mineralized Chitosan/Carbon Nanotubes (G5/C+CNTsM) and Mineralized Chitosan/Carbon Nanotubes with LLLT (G6/C+CNTsM+L). After 5 weeks, the biocompatibility of the chitosan/carbon nanotubes scaffolds was observed, with the absence of inflammatory infiltrates and fibrotic tissue. Bone neoformation was denser, thicker and voluminous in G6/C+CNTsM+L. Histomorphometric analyses showed that the relative percentage and standard deviations (mean ± SD) of new bone formation in groups G1 to G6 were 59.93 ± 3.04a (G1/Control), 70.83 ± 1.21b (G2/Laser), 70.09 ± 4.31b (G3/C+CNTs), 81.6 ± 5.74c (G4/C+CNTs+L), 81.4 ± 4.57c (G5/C+CNTsM) and 91.3 ± 4.81d (G6/C+CNTsM+L), respectively, with G6 showing a significant difference in relation to the other groups (a ≠ b ≠ c ≠ d; p < 0.05). Immunohistochemistry also revealed good expression of osteocalcin (OC), osteopontin (OP) and vascular endothelial growth factor (VEGF). It was concluded that chitosan-based carbon nanotube materials combined with LLLT effectively stimulated the bone healing process. |
format | Online Article Text |
id | pubmed-9223695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92236952022-06-24 Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation Silva, Samantha Ketelyn Plepis, Ana Maria Guzzi Martins, Virginia da Conceição Amaro Horn, Marilia Marta Buchaim, Daniela Vieira Buchaim, Rogerio Leone Pelegrine, André Antônio Silva, Vinícius Rodrigues Kudo, Mateus Hissashi Matsumoto Fernandes, José Francisco Rebello Nazari, Fabricio Montenegro da Cunha, Marcelo Rodrigues Int J Mol Sci Article Biomaterials have been investigated as an alternative for the treatment of bone defects, such as chitosan/carbon nanotubes scaffolds, which allow cell proliferation. However, bone regeneration can be accelerated by electrotherapeutic resources that act on bone metabolism, such as low-level laser therapy (LLLT). Thus, this study evaluated the regeneration of bone lesions grafted with chitosan/carbon nanotubes scaffolds and associated with LLLT. For this, a defect (3 mm) was created in the femur of thirty rats, which were divided into 6 groups: Control (G1/Control), LLLT (G2/Laser), Chitosan/Carbon Nanotubes (G3/C+CNTs), Chitosan/Carbon Nanotubes with LLLT (G4/C+CNTs+L), Mineralized Chitosan/Carbon Nanotubes (G5/C+CNTsM) and Mineralized Chitosan/Carbon Nanotubes with LLLT (G6/C+CNTsM+L). After 5 weeks, the biocompatibility of the chitosan/carbon nanotubes scaffolds was observed, with the absence of inflammatory infiltrates and fibrotic tissue. Bone neoformation was denser, thicker and voluminous in G6/C+CNTsM+L. Histomorphometric analyses showed that the relative percentage and standard deviations (mean ± SD) of new bone formation in groups G1 to G6 were 59.93 ± 3.04a (G1/Control), 70.83 ± 1.21b (G2/Laser), 70.09 ± 4.31b (G3/C+CNTs), 81.6 ± 5.74c (G4/C+CNTs+L), 81.4 ± 4.57c (G5/C+CNTsM) and 91.3 ± 4.81d (G6/C+CNTsM+L), respectively, with G6 showing a significant difference in relation to the other groups (a ≠ b ≠ c ≠ d; p < 0.05). Immunohistochemistry also revealed good expression of osteocalcin (OC), osteopontin (OP) and vascular endothelial growth factor (VEGF). It was concluded that chitosan-based carbon nanotube materials combined with LLLT effectively stimulated the bone healing process. MDPI 2022-06-10 /pmc/articles/PMC9223695/ /pubmed/35742948 http://dx.doi.org/10.3390/ijms23126503 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Silva, Samantha Ketelyn Plepis, Ana Maria Guzzi Martins, Virginia da Conceição Amaro Horn, Marilia Marta Buchaim, Daniela Vieira Buchaim, Rogerio Leone Pelegrine, André Antônio Silva, Vinícius Rodrigues Kudo, Mateus Hissashi Matsumoto Fernandes, José Francisco Rebello Nazari, Fabricio Montenegro da Cunha, Marcelo Rodrigues Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation |
title | Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation |
title_full | Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation |
title_fullStr | Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation |
title_full_unstemmed | Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation |
title_short | Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation |
title_sort | suitability of chitosan scaffolds with carbon nanotubes for bone defects treated with photobiomodulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223695/ https://www.ncbi.nlm.nih.gov/pubmed/35742948 http://dx.doi.org/10.3390/ijms23126503 |
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