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Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis
Central nervous system (CNS) tuberculosis is the most lethal and devastating form among the diseases caused by Mycobacterium tuberculosis. The mechanisms by which M. tuberculosis bacilli enter the CNS are still unclear. However, the BBB and the BCSFB have been proposed as possible routes of access i...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223849/ https://www.ncbi.nlm.nih.gov/pubmed/35742886 http://dx.doi.org/10.3390/ijms23126436 |
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author | Sánchez-Garibay, Carlos Salinas-Lara, Citlaltepetl Gómez-López, Marcos Artemio Soto-Rojas, Luis O. Castillón-Benavides, Nidia Karen Castillón-Benavides, Omar Jorge Hernández-Campos, María Elena Hernández-Pando, Rogelio Marquina-Castillo, Brenda Flores-Barrada, Manuel Alejandro Choreño-Parra, José Alberto León-Contreras, Juan Carlos Tena-Suck, Martha Lilia Mata-Espinosa, Dulce Adriana Nava, Porfirio Medina-Mendoza, Jessica Rodríguez-Balderas, Cesar Augusto |
author_facet | Sánchez-Garibay, Carlos Salinas-Lara, Citlaltepetl Gómez-López, Marcos Artemio Soto-Rojas, Luis O. Castillón-Benavides, Nidia Karen Castillón-Benavides, Omar Jorge Hernández-Campos, María Elena Hernández-Pando, Rogelio Marquina-Castillo, Brenda Flores-Barrada, Manuel Alejandro Choreño-Parra, José Alberto León-Contreras, Juan Carlos Tena-Suck, Martha Lilia Mata-Espinosa, Dulce Adriana Nava, Porfirio Medina-Mendoza, Jessica Rodríguez-Balderas, Cesar Augusto |
author_sort | Sánchez-Garibay, Carlos |
collection | PubMed |
description | Central nervous system (CNS) tuberculosis is the most lethal and devastating form among the diseases caused by Mycobacterium tuberculosis. The mechanisms by which M. tuberculosis bacilli enter the CNS are still unclear. However, the BBB and the BCSFB have been proposed as possible routes of access into the brain. We previously reported that certain strains of M. tuberculosis possess an enhanced ability to cause secondary CNS infection in a mouse model of progressive pulmonary tuberculosis. Here, we evaluated the morphostructural and molecular integrity of CNS barriers. For this purpose, we analyzed through transmission electron microscopy the ultrastructure of brain parenchymal microvessels and choroid plexus epithelium from animals infected with two mycobacterial strains. Additionally, we determined the expression of junctional proteins and cytokines by immunological techniques. The results showed that the presence of M. tuberculosis induced disruption of the BCSFB but no disruption of the BBB, and that the severity of such damage was related to the strain used, suggesting that variations in the ability to cause CNS disease among distinct strains of bacteria may also be linked to their capacity to cause direct or indirect disruption of these barriers. Understanding the pathophysiological mechanisms involved in CNS tuberculosis may facilitate the establishment of new biomarkers and therapeutic targets. |
format | Online Article Text |
id | pubmed-9223849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92238492022-06-24 Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis Sánchez-Garibay, Carlos Salinas-Lara, Citlaltepetl Gómez-López, Marcos Artemio Soto-Rojas, Luis O. Castillón-Benavides, Nidia Karen Castillón-Benavides, Omar Jorge Hernández-Campos, María Elena Hernández-Pando, Rogelio Marquina-Castillo, Brenda Flores-Barrada, Manuel Alejandro Choreño-Parra, José Alberto León-Contreras, Juan Carlos Tena-Suck, Martha Lilia Mata-Espinosa, Dulce Adriana Nava, Porfirio Medina-Mendoza, Jessica Rodríguez-Balderas, Cesar Augusto Int J Mol Sci Article Central nervous system (CNS) tuberculosis is the most lethal and devastating form among the diseases caused by Mycobacterium tuberculosis. The mechanisms by which M. tuberculosis bacilli enter the CNS are still unclear. However, the BBB and the BCSFB have been proposed as possible routes of access into the brain. We previously reported that certain strains of M. tuberculosis possess an enhanced ability to cause secondary CNS infection in a mouse model of progressive pulmonary tuberculosis. Here, we evaluated the morphostructural and molecular integrity of CNS barriers. For this purpose, we analyzed through transmission electron microscopy the ultrastructure of brain parenchymal microvessels and choroid plexus epithelium from animals infected with two mycobacterial strains. Additionally, we determined the expression of junctional proteins and cytokines by immunological techniques. The results showed that the presence of M. tuberculosis induced disruption of the BCSFB but no disruption of the BBB, and that the severity of such damage was related to the strain used, suggesting that variations in the ability to cause CNS disease among distinct strains of bacteria may also be linked to their capacity to cause direct or indirect disruption of these barriers. Understanding the pathophysiological mechanisms involved in CNS tuberculosis may facilitate the establishment of new biomarkers and therapeutic targets. MDPI 2022-06-09 /pmc/articles/PMC9223849/ /pubmed/35742886 http://dx.doi.org/10.3390/ijms23126436 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sánchez-Garibay, Carlos Salinas-Lara, Citlaltepetl Gómez-López, Marcos Artemio Soto-Rojas, Luis O. Castillón-Benavides, Nidia Karen Castillón-Benavides, Omar Jorge Hernández-Campos, María Elena Hernández-Pando, Rogelio Marquina-Castillo, Brenda Flores-Barrada, Manuel Alejandro Choreño-Parra, José Alberto León-Contreras, Juan Carlos Tena-Suck, Martha Lilia Mata-Espinosa, Dulce Adriana Nava, Porfirio Medina-Mendoza, Jessica Rodríguez-Balderas, Cesar Augusto Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis |
title | Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis |
title_full | Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis |
title_fullStr | Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis |
title_full_unstemmed | Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis |
title_short | Mycobacterium tuberculosis Infection Induces BCSFB Disruption but No BBB Disruption In Vivo: Implications in the Pathophysiology of Tuberculous Meningitis |
title_sort | mycobacterium tuberculosis infection induces bcsfb disruption but no bbb disruption in vivo: implications in the pathophysiology of tuberculous meningitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223849/ https://www.ncbi.nlm.nih.gov/pubmed/35742886 http://dx.doi.org/10.3390/ijms23126436 |
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