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The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats

Anemia is a commonly observed consequence of whole-body exposure to a dose of X-ray or gamma irradiation of the order of the mean lethal dose in mammals, and it is an important factor for the determination of the survival of animals. The aim of this study was to unravel the effect of laser-driven ul...

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Autores principales: Tsakanova, Gohar, Avetisyan, Aida, Karalova, Elena, Abroyan, Liana, Hakobyan, Lina, Semerjyan, Anna, Karalyan, Naira, Arakelova, Elina, Ayvazyan, Violetta, Matevosyan, Lusine, Navasardyan, Arpine, Ayvazyan, Anna, Davtyan, Hakob, Grigoryan, Bagrat, Arakelyan, Arsen, Karalyan, Zaven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223873/
https://www.ncbi.nlm.nih.gov/pubmed/35743135
http://dx.doi.org/10.3390/ijms23126692
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author Tsakanova, Gohar
Avetisyan, Aida
Karalova, Elena
Abroyan, Liana
Hakobyan, Lina
Semerjyan, Anna
Karalyan, Naira
Arakelova, Elina
Ayvazyan, Violetta
Matevosyan, Lusine
Navasardyan, Arpine
Ayvazyan, Anna
Davtyan, Hakob
Grigoryan, Bagrat
Arakelyan, Arsen
Karalyan, Zaven
author_facet Tsakanova, Gohar
Avetisyan, Aida
Karalova, Elena
Abroyan, Liana
Hakobyan, Lina
Semerjyan, Anna
Karalyan, Naira
Arakelova, Elina
Ayvazyan, Violetta
Matevosyan, Lusine
Navasardyan, Arpine
Ayvazyan, Anna
Davtyan, Hakob
Grigoryan, Bagrat
Arakelyan, Arsen
Karalyan, Zaven
author_sort Tsakanova, Gohar
collection PubMed
description Anemia is a commonly observed consequence of whole-body exposure to a dose of X-ray or gamma irradiation of the order of the mean lethal dose in mammals, and it is an important factor for the determination of the survival of animals. The aim of this study was to unravel the effect of laser-driven ultrashort pulsed electron beam (UPEB) irradiation on the process of erythropoiesis and the redox state in the organism. Wistar rats were exposed to laser-driven UPEB irradiation, after which the level of oxidative stress and the activities of different antioxidant enzymes, as well as blood smears, bone marrow imprints and sections, erythroblastic islets, hemoglobin and hematocrit, hepatic iron, DNA, and erythropoietin levels, were assessed on the 1st, 3rd, 7th, 14th, and 28th days after irradiation. Despite the fact that laser-driven UPEB irradiation requires quite low doses and repetition rates to achieve the LD(50) in rats, our findings suggest that whole-body exposure with this new type of irradiation causes relatively mild anemia in rats, with subsequent fast recovery up to the 28th day. Moreover, this novel type of irradiation causes highly intense processes of oxidative stress, which, despite being relatively extinguished, did not reach the physiologically stable level even at the 28th day after irradiation due to the violations in the antioxidant system of the organism.
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spelling pubmed-92238732022-06-24 The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats Tsakanova, Gohar Avetisyan, Aida Karalova, Elena Abroyan, Liana Hakobyan, Lina Semerjyan, Anna Karalyan, Naira Arakelova, Elina Ayvazyan, Violetta Matevosyan, Lusine Navasardyan, Arpine Ayvazyan, Anna Davtyan, Hakob Grigoryan, Bagrat Arakelyan, Arsen Karalyan, Zaven Int J Mol Sci Article Anemia is a commonly observed consequence of whole-body exposure to a dose of X-ray or gamma irradiation of the order of the mean lethal dose in mammals, and it is an important factor for the determination of the survival of animals. The aim of this study was to unravel the effect of laser-driven ultrashort pulsed electron beam (UPEB) irradiation on the process of erythropoiesis and the redox state in the organism. Wistar rats were exposed to laser-driven UPEB irradiation, after which the level of oxidative stress and the activities of different antioxidant enzymes, as well as blood smears, bone marrow imprints and sections, erythroblastic islets, hemoglobin and hematocrit, hepatic iron, DNA, and erythropoietin levels, were assessed on the 1st, 3rd, 7th, 14th, and 28th days after irradiation. Despite the fact that laser-driven UPEB irradiation requires quite low doses and repetition rates to achieve the LD(50) in rats, our findings suggest that whole-body exposure with this new type of irradiation causes relatively mild anemia in rats, with subsequent fast recovery up to the 28th day. Moreover, this novel type of irradiation causes highly intense processes of oxidative stress, which, despite being relatively extinguished, did not reach the physiologically stable level even at the 28th day after irradiation due to the violations in the antioxidant system of the organism. MDPI 2022-06-15 /pmc/articles/PMC9223873/ /pubmed/35743135 http://dx.doi.org/10.3390/ijms23126692 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsakanova, Gohar
Avetisyan, Aida
Karalova, Elena
Abroyan, Liana
Hakobyan, Lina
Semerjyan, Anna
Karalyan, Naira
Arakelova, Elina
Ayvazyan, Violetta
Matevosyan, Lusine
Navasardyan, Arpine
Ayvazyan, Anna
Davtyan, Hakob
Grigoryan, Bagrat
Arakelyan, Arsen
Karalyan, Zaven
The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats
title The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats
title_full The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats
title_fullStr The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats
title_full_unstemmed The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats
title_short The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats
title_sort effect of low-energy laser-driven ultrashort pulsed electron beam irradiation on erythropoiesis and oxidative stress in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223873/
https://www.ncbi.nlm.nih.gov/pubmed/35743135
http://dx.doi.org/10.3390/ijms23126692
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