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IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis
Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well unders...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224118/ https://www.ncbi.nlm.nih.gov/pubmed/35743247 http://dx.doi.org/10.3390/ijms23126804 |
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author | Wu, Tsung-Ju Chang, Sunny Li-Yun Lin, Chih-Yang Lai, Chao-Yang He, Xiu-Yuan Tsai, Chun-Hao Ko, Chih-Yuan Fong, Yi-Chin Su, Chen-Ming Tang, Chih-Hsin |
author_facet | Wu, Tsung-Ju Chang, Sunny Li-Yun Lin, Chih-Yang Lai, Chao-Yang He, Xiu-Yuan Tsai, Chun-Hao Ko, Chih-Yuan Fong, Yi-Chin Su, Chen-Ming Tang, Chih-Hsin |
author_sort | Wu, Tsung-Ju |
collection | PubMed |
description | Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well understood. Our analysis of synovial tissue samples retrieved from the Gene Expression Omnibus (GEO) dataset exhibited higher monocyte marker (CD11b) and vascular cell adhesion molecule 1 (VCAM-1) levels in OA samples than in normal, healthy samples. The stimulation of human OA synovial fibroblasts (OASFs) with IL-17 increased VCAM-1 production and subsequently enhanced monocyte adhesion. IL-17 affected VCAM-1-dependent monocyte adhesion by reducing miR-5701 expression through the protein kinase C (PKC)-α and c-Jun N-terminal kinase (JNK) signaling cascades. Our findings improve our understanding about the effect of IL-17 on OA progression and, in particular, VCAM-1 production and monocyte adhesion, which may help with the design of more effective OA treatments. |
format | Online Article Text |
id | pubmed-9224118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92241182022-06-24 IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis Wu, Tsung-Ju Chang, Sunny Li-Yun Lin, Chih-Yang Lai, Chao-Yang He, Xiu-Yuan Tsai, Chun-Hao Ko, Chih-Yuan Fong, Yi-Chin Su, Chen-Ming Tang, Chih-Hsin Int J Mol Sci Article Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well understood. Our analysis of synovial tissue samples retrieved from the Gene Expression Omnibus (GEO) dataset exhibited higher monocyte marker (CD11b) and vascular cell adhesion molecule 1 (VCAM-1) levels in OA samples than in normal, healthy samples. The stimulation of human OA synovial fibroblasts (OASFs) with IL-17 increased VCAM-1 production and subsequently enhanced monocyte adhesion. IL-17 affected VCAM-1-dependent monocyte adhesion by reducing miR-5701 expression through the protein kinase C (PKC)-α and c-Jun N-terminal kinase (JNK) signaling cascades. Our findings improve our understanding about the effect of IL-17 on OA progression and, in particular, VCAM-1 production and monocyte adhesion, which may help with the design of more effective OA treatments. MDPI 2022-06-18 /pmc/articles/PMC9224118/ /pubmed/35743247 http://dx.doi.org/10.3390/ijms23126804 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wu, Tsung-Ju Chang, Sunny Li-Yun Lin, Chih-Yang Lai, Chao-Yang He, Xiu-Yuan Tsai, Chun-Hao Ko, Chih-Yuan Fong, Yi-Chin Su, Chen-Ming Tang, Chih-Hsin IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis |
title | IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis |
title_full | IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis |
title_fullStr | IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis |
title_full_unstemmed | IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis |
title_short | IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis |
title_sort | il-17 facilitates vcam-1 production and monocyte adhesion in osteoarthritis synovial fibroblasts by suppressing mir-5701 synthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224118/ https://www.ncbi.nlm.nih.gov/pubmed/35743247 http://dx.doi.org/10.3390/ijms23126804 |
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