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IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis

Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well unders...

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Autores principales: Wu, Tsung-Ju, Chang, Sunny Li-Yun, Lin, Chih-Yang, Lai, Chao-Yang, He, Xiu-Yuan, Tsai, Chun-Hao, Ko, Chih-Yuan, Fong, Yi-Chin, Su, Chen-Ming, Tang, Chih-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224118/
https://www.ncbi.nlm.nih.gov/pubmed/35743247
http://dx.doi.org/10.3390/ijms23126804
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author Wu, Tsung-Ju
Chang, Sunny Li-Yun
Lin, Chih-Yang
Lai, Chao-Yang
He, Xiu-Yuan
Tsai, Chun-Hao
Ko, Chih-Yuan
Fong, Yi-Chin
Su, Chen-Ming
Tang, Chih-Hsin
author_facet Wu, Tsung-Ju
Chang, Sunny Li-Yun
Lin, Chih-Yang
Lai, Chao-Yang
He, Xiu-Yuan
Tsai, Chun-Hao
Ko, Chih-Yuan
Fong, Yi-Chin
Su, Chen-Ming
Tang, Chih-Hsin
author_sort Wu, Tsung-Ju
collection PubMed
description Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well understood. Our analysis of synovial tissue samples retrieved from the Gene Expression Omnibus (GEO) dataset exhibited higher monocyte marker (CD11b) and vascular cell adhesion molecule 1 (VCAM-1) levels in OA samples than in normal, healthy samples. The stimulation of human OA synovial fibroblasts (OASFs) with IL-17 increased VCAM-1 production and subsequently enhanced monocyte adhesion. IL-17 affected VCAM-1-dependent monocyte adhesion by reducing miR-5701 expression through the protein kinase C (PKC)-α and c-Jun N-terminal kinase (JNK) signaling cascades. Our findings improve our understanding about the effect of IL-17 on OA progression and, in particular, VCAM-1 production and monocyte adhesion, which may help with the design of more effective OA treatments.
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spelling pubmed-92241182022-06-24 IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis Wu, Tsung-Ju Chang, Sunny Li-Yun Lin, Chih-Yang Lai, Chao-Yang He, Xiu-Yuan Tsai, Chun-Hao Ko, Chih-Yuan Fong, Yi-Chin Su, Chen-Ming Tang, Chih-Hsin Int J Mol Sci Article Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well understood. Our analysis of synovial tissue samples retrieved from the Gene Expression Omnibus (GEO) dataset exhibited higher monocyte marker (CD11b) and vascular cell adhesion molecule 1 (VCAM-1) levels in OA samples than in normal, healthy samples. The stimulation of human OA synovial fibroblasts (OASFs) with IL-17 increased VCAM-1 production and subsequently enhanced monocyte adhesion. IL-17 affected VCAM-1-dependent monocyte adhesion by reducing miR-5701 expression through the protein kinase C (PKC)-α and c-Jun N-terminal kinase (JNK) signaling cascades. Our findings improve our understanding about the effect of IL-17 on OA progression and, in particular, VCAM-1 production and monocyte adhesion, which may help with the design of more effective OA treatments. MDPI 2022-06-18 /pmc/articles/PMC9224118/ /pubmed/35743247 http://dx.doi.org/10.3390/ijms23126804 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Tsung-Ju
Chang, Sunny Li-Yun
Lin, Chih-Yang
Lai, Chao-Yang
He, Xiu-Yuan
Tsai, Chun-Hao
Ko, Chih-Yuan
Fong, Yi-Chin
Su, Chen-Ming
Tang, Chih-Hsin
IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis
title IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis
title_full IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis
title_fullStr IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis
title_full_unstemmed IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis
title_short IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis
title_sort il-17 facilitates vcam-1 production and monocyte adhesion in osteoarthritis synovial fibroblasts by suppressing mir-5701 synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224118/
https://www.ncbi.nlm.nih.gov/pubmed/35743247
http://dx.doi.org/10.3390/ijms23126804
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