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Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication

Canine circovirus (CanineCV) is an emerging virus in canines. Since the first strain of CanineCV was reported in 2012, CanineCV infection has shown a trend toward becoming a global epidemic. CanineCV infection often occurs with coinfection with other pathogens that may aggravate the symptoms of dise...

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Autores principales: Hao, Xiangqi, Li, Yanchao, Chen, Hui, Chen, Bo, Liu, Ruohan, Wu, Yidan, Xiao, Xiangyu, Zhou, Pei, Li, Shoujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224199/
https://www.ncbi.nlm.nih.gov/pubmed/35742826
http://dx.doi.org/10.3390/ijms23126382
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author Hao, Xiangqi
Li, Yanchao
Chen, Hui
Chen, Bo
Liu, Ruohan
Wu, Yidan
Xiao, Xiangyu
Zhou, Pei
Li, Shoujun
author_facet Hao, Xiangqi
Li, Yanchao
Chen, Hui
Chen, Bo
Liu, Ruohan
Wu, Yidan
Xiao, Xiangyu
Zhou, Pei
Li, Shoujun
author_sort Hao, Xiangqi
collection PubMed
description Canine circovirus (CanineCV) is an emerging virus in canines. Since the first strain of CanineCV was reported in 2012, CanineCV infection has shown a trend toward becoming a global epidemic. CanineCV infection often occurs with coinfection with other pathogens that may aggravate the symptoms of disease in affected dogs. Currently, CanineCV has not been successfully isolated by laboratories, resulting in a lack of clarity regarding its physicochemical properties, replication process, and pathogenic characteristics. To address this knowledge gap, the following results were obtained in this study. First, a CanineCV strain was rescued in F81 cells using infectious clone plasmids. Second, the Rep protein produced by the viral packaging rescue process was found to be associated with cytopathic effects. Additionally, the Rep protein and CanineCV inhibited the activation of the type I interferon (IFN-I) promoter, blocking subsequent expression of interferon-stimulated genes (ISGs). Furthermore, Rep was found to broadly inhibit host protein expression. We speculate that in CanineCV and canine parvovirus type 2 (CPV-2) coinfection cases, CanineCV promotes CPV-2 replication by inducing immunosuppression, which may increase the severity of clinical symptoms.
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spelling pubmed-92241992022-06-24 Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication Hao, Xiangqi Li, Yanchao Chen, Hui Chen, Bo Liu, Ruohan Wu, Yidan Xiao, Xiangyu Zhou, Pei Li, Shoujun Int J Mol Sci Article Canine circovirus (CanineCV) is an emerging virus in canines. Since the first strain of CanineCV was reported in 2012, CanineCV infection has shown a trend toward becoming a global epidemic. CanineCV infection often occurs with coinfection with other pathogens that may aggravate the symptoms of disease in affected dogs. Currently, CanineCV has not been successfully isolated by laboratories, resulting in a lack of clarity regarding its physicochemical properties, replication process, and pathogenic characteristics. To address this knowledge gap, the following results were obtained in this study. First, a CanineCV strain was rescued in F81 cells using infectious clone plasmids. Second, the Rep protein produced by the viral packaging rescue process was found to be associated with cytopathic effects. Additionally, the Rep protein and CanineCV inhibited the activation of the type I interferon (IFN-I) promoter, blocking subsequent expression of interferon-stimulated genes (ISGs). Furthermore, Rep was found to broadly inhibit host protein expression. We speculate that in CanineCV and canine parvovirus type 2 (CPV-2) coinfection cases, CanineCV promotes CPV-2 replication by inducing immunosuppression, which may increase the severity of clinical symptoms. MDPI 2022-06-07 /pmc/articles/PMC9224199/ /pubmed/35742826 http://dx.doi.org/10.3390/ijms23126382 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hao, Xiangqi
Li, Yanchao
Chen, Hui
Chen, Bo
Liu, Ruohan
Wu, Yidan
Xiao, Xiangyu
Zhou, Pei
Li, Shoujun
Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication
title Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication
title_full Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication
title_fullStr Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication
title_full_unstemmed Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication
title_short Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication
title_sort canine circovirus suppresses the type i interferon response and protein expression but promotes cpv-2 replication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224199/
https://www.ncbi.nlm.nih.gov/pubmed/35742826
http://dx.doi.org/10.3390/ijms23126382
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