Cargando…
Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content
Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays a vital role in photosynthesis. However, the reported knowledge of LHCs in kiwifruit is inadequate and poorly understood. In this study, we identified 42 and 45 LHC genes in Actinidia chinensis (Ac) and A. eriantha (A...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224368/ https://www.ncbi.nlm.nih.gov/pubmed/35742967 http://dx.doi.org/10.3390/ijms23126528 |
_version_ | 1784733346973089792 |
---|---|
author | Luo, Juan Abid, Muhammad Tu, Jing Gao, Puxing Wang, Zupeng Huang, Hongwen |
author_facet | Luo, Juan Abid, Muhammad Tu, Jing Gao, Puxing Wang, Zupeng Huang, Hongwen |
author_sort | Luo, Juan |
collection | PubMed |
description | Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays a vital role in photosynthesis. However, the reported knowledge of LHCs in kiwifruit is inadequate and poorly understood. In this study, we identified 42 and 45 LHC genes in Actinidia chinensis (Ac) and A. eriantha (Ae) genomes. Phylogenetic analysis showed that the kiwifruit LHCs of both species were grouped into four subfamilies (Lhc, Lil, PsbS, and FCII). Expression profiles and qRT-PCR results revealed expression levels of LHC genes closely related to the light, temperature fluctuations, color changes during fruit ripening, and kiwifruit responses to Pseudomonas syringae pv. actinidiae (Psa). Subcellular localization analysis showed that AcLhcb1.5/3.1/3.2 were localized in the chloroplast while transient overexpression of AcLhcb3.1/3.2 in tobacco leaves confirmed a significantly increased content of chlorophyll a. Our findings provide evidence of the characters and evolution patterns of kiwifruit LHCs genes in kiwifruit and verify the AcLhcb3.1/3.2 genes controlling the chlorophyll a content. |
format | Online Article Text |
id | pubmed-9224368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92243682022-06-24 Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content Luo, Juan Abid, Muhammad Tu, Jing Gao, Puxing Wang, Zupeng Huang, Hongwen Int J Mol Sci Article Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays a vital role in photosynthesis. However, the reported knowledge of LHCs in kiwifruit is inadequate and poorly understood. In this study, we identified 42 and 45 LHC genes in Actinidia chinensis (Ac) and A. eriantha (Ae) genomes. Phylogenetic analysis showed that the kiwifruit LHCs of both species were grouped into four subfamilies (Lhc, Lil, PsbS, and FCII). Expression profiles and qRT-PCR results revealed expression levels of LHC genes closely related to the light, temperature fluctuations, color changes during fruit ripening, and kiwifruit responses to Pseudomonas syringae pv. actinidiae (Psa). Subcellular localization analysis showed that AcLhcb1.5/3.1/3.2 were localized in the chloroplast while transient overexpression of AcLhcb3.1/3.2 in tobacco leaves confirmed a significantly increased content of chlorophyll a. Our findings provide evidence of the characters and evolution patterns of kiwifruit LHCs genes in kiwifruit and verify the AcLhcb3.1/3.2 genes controlling the chlorophyll a content. MDPI 2022-06-10 /pmc/articles/PMC9224368/ /pubmed/35742967 http://dx.doi.org/10.3390/ijms23126528 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luo, Juan Abid, Muhammad Tu, Jing Gao, Puxing Wang, Zupeng Huang, Hongwen Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content |
title | Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content |
title_full | Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content |
title_fullStr | Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content |
title_full_unstemmed | Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content |
title_short | Genome-Wide Identification of the LHC Gene Family in Kiwifruit and Regulatory Role of AcLhcb3.1/3.2 for Chlorophyll a Content |
title_sort | genome-wide identification of the lhc gene family in kiwifruit and regulatory role of aclhcb3.1/3.2 for chlorophyll a content |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224368/ https://www.ncbi.nlm.nih.gov/pubmed/35742967 http://dx.doi.org/10.3390/ijms23126528 |
work_keys_str_mv | AT luojuan genomewideidentificationofthelhcgenefamilyinkiwifruitandregulatoryroleofaclhcb3132forchlorophyllacontent AT abidmuhammad genomewideidentificationofthelhcgenefamilyinkiwifruitandregulatoryroleofaclhcb3132forchlorophyllacontent AT tujing genomewideidentificationofthelhcgenefamilyinkiwifruitandregulatoryroleofaclhcb3132forchlorophyllacontent AT gaopuxing genomewideidentificationofthelhcgenefamilyinkiwifruitandregulatoryroleofaclhcb3132forchlorophyllacontent AT wangzupeng genomewideidentificationofthelhcgenefamilyinkiwifruitandregulatoryroleofaclhcb3132forchlorophyllacontent AT huanghongwen genomewideidentificationofthelhcgenefamilyinkiwifruitandregulatoryroleofaclhcb3132forchlorophyllacontent |