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Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain
A new generation of optogenetic tools for analyzing neural activity has been contributing to the elucidation of classical open questions in neuroscience. Specifically, voltage imaging technologies using enhanced genetically encoded voltage indicators have been increasingly used to observe the dynami...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224488/ https://www.ncbi.nlm.nih.gov/pubmed/35743257 http://dx.doi.org/10.3390/ijms23126814 |
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author | Pedrosa, Rafael Song, Chenchen Knöpfel, Thomas Battaglia, Francesco |
author_facet | Pedrosa, Rafael Song, Chenchen Knöpfel, Thomas Battaglia, Francesco |
author_sort | Pedrosa, Rafael |
collection | PubMed |
description | A new generation of optogenetic tools for analyzing neural activity has been contributing to the elucidation of classical open questions in neuroscience. Specifically, voltage imaging technologies using enhanced genetically encoded voltage indicators have been increasingly used to observe the dynamics of large circuits at the mesoscale. Here, we describe how to combine cortical wide-field voltage imaging with hippocampal electrophysiology in awake, behaving mice. Furthermore, we highlight how this method can be useful for different possible investigations, using the characterization of hippocampal–neocortical interactions as a case study. |
format | Online Article Text |
id | pubmed-9224488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92244882022-06-24 Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain Pedrosa, Rafael Song, Chenchen Knöpfel, Thomas Battaglia, Francesco Int J Mol Sci Article A new generation of optogenetic tools for analyzing neural activity has been contributing to the elucidation of classical open questions in neuroscience. Specifically, voltage imaging technologies using enhanced genetically encoded voltage indicators have been increasingly used to observe the dynamics of large circuits at the mesoscale. Here, we describe how to combine cortical wide-field voltage imaging with hippocampal electrophysiology in awake, behaving mice. Furthermore, we highlight how this method can be useful for different possible investigations, using the characterization of hippocampal–neocortical interactions as a case study. MDPI 2022-06-19 /pmc/articles/PMC9224488/ /pubmed/35743257 http://dx.doi.org/10.3390/ijms23126814 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pedrosa, Rafael Song, Chenchen Knöpfel, Thomas Battaglia, Francesco Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain |
title | Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain |
title_full | Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain |
title_fullStr | Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain |
title_full_unstemmed | Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain |
title_short | Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain |
title_sort | combining cortical voltage imaging and hippocampal electrophysiology for investigating global, multi-timescale activity interactions in the brain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224488/ https://www.ncbi.nlm.nih.gov/pubmed/35743257 http://dx.doi.org/10.3390/ijms23126814 |
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