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Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain

A new generation of optogenetic tools for analyzing neural activity has been contributing to the elucidation of classical open questions in neuroscience. Specifically, voltage imaging technologies using enhanced genetically encoded voltage indicators have been increasingly used to observe the dynami...

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Detalles Bibliográficos
Autores principales: Pedrosa, Rafael, Song, Chenchen, Knöpfel, Thomas, Battaglia, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224488/
https://www.ncbi.nlm.nih.gov/pubmed/35743257
http://dx.doi.org/10.3390/ijms23126814
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author Pedrosa, Rafael
Song, Chenchen
Knöpfel, Thomas
Battaglia, Francesco
author_facet Pedrosa, Rafael
Song, Chenchen
Knöpfel, Thomas
Battaglia, Francesco
author_sort Pedrosa, Rafael
collection PubMed
description A new generation of optogenetic tools for analyzing neural activity has been contributing to the elucidation of classical open questions in neuroscience. Specifically, voltage imaging technologies using enhanced genetically encoded voltage indicators have been increasingly used to observe the dynamics of large circuits at the mesoscale. Here, we describe how to combine cortical wide-field voltage imaging with hippocampal electrophysiology in awake, behaving mice. Furthermore, we highlight how this method can be useful for different possible investigations, using the characterization of hippocampal–neocortical interactions as a case study.
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spelling pubmed-92244882022-06-24 Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain Pedrosa, Rafael Song, Chenchen Knöpfel, Thomas Battaglia, Francesco Int J Mol Sci Article A new generation of optogenetic tools for analyzing neural activity has been contributing to the elucidation of classical open questions in neuroscience. Specifically, voltage imaging technologies using enhanced genetically encoded voltage indicators have been increasingly used to observe the dynamics of large circuits at the mesoscale. Here, we describe how to combine cortical wide-field voltage imaging with hippocampal electrophysiology in awake, behaving mice. Furthermore, we highlight how this method can be useful for different possible investigations, using the characterization of hippocampal–neocortical interactions as a case study. MDPI 2022-06-19 /pmc/articles/PMC9224488/ /pubmed/35743257 http://dx.doi.org/10.3390/ijms23126814 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pedrosa, Rafael
Song, Chenchen
Knöpfel, Thomas
Battaglia, Francesco
Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain
title Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain
title_full Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain
title_fullStr Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain
title_full_unstemmed Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain
title_short Combining Cortical Voltage Imaging and Hippocampal Electrophysiology for Investigating Global, Multi-Timescale Activity Interactions in the Brain
title_sort combining cortical voltage imaging and hippocampal electrophysiology for investigating global, multi-timescale activity interactions in the brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224488/
https://www.ncbi.nlm.nih.gov/pubmed/35743257
http://dx.doi.org/10.3390/ijms23126814
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