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Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons

Nurr1 and brain-derived neurotrophic factor (BDNF) play major roles in cognition. Nurr1 regulates BDNF in midbrain dopaminergic neurons and cerebellar granule cells. Nurr1 and BDNF are also highly expressed in the cerebral cortex, a brain area important in cognition. Due to Nurr1 and BDNF tissue spe...

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Autores principales: Abdollahi, Mona, Fahnestock, Margaret
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224520/
https://www.ncbi.nlm.nih.gov/pubmed/35743300
http://dx.doi.org/10.3390/ijms23126853
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author Abdollahi, Mona
Fahnestock, Margaret
author_facet Abdollahi, Mona
Fahnestock, Margaret
author_sort Abdollahi, Mona
collection PubMed
description Nurr1 and brain-derived neurotrophic factor (BDNF) play major roles in cognition. Nurr1 regulates BDNF in midbrain dopaminergic neurons and cerebellar granule cells. Nurr1 and BDNF are also highly expressed in the cerebral cortex, a brain area important in cognition. Due to Nurr1 and BDNF tissue specificity, the regulatory effect of Nurr1 on BDNF in different brain areas cannot be generalized. The relationship between Nurr1 and BDNF in the cortex has not been investigated previously. Therefore, we examined Nurr1-mediated BDNF regulation in cortical neurons in activity-dependent and activity-independent states. Mouse primary cortical neurons were treated with the Nurr1 agonist, amodiaquine (AQ). Membrane depolarization was induced by KCl or veratridine and reversed by nimodipine. AQ and membrane depolarization significantly increased Nurr1 (p < 0.001) and BDNF (p(AQ) < 0.001, p(KCl) < 0.01) as assessed by real-time qRT-PCR. However, Nurr1 knockdown did not affect BDNF gene expression in resting or depolarized neurons. Accordingly, the positive correlation between Nurr1 and BDNF expression in AQ and membrane depolarization experiments does not imply co-regulation because Nurr1 knockdown did not affect BDNF gene expression in resting or depolarized cortical neurons. Therefore, in contrast to midbrain dopaminergic neurons and cerebellar granule cells, Nurr1 does not regulate BDNF in cortical neurons.
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spelling pubmed-92245202022-06-24 Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons Abdollahi, Mona Fahnestock, Margaret Int J Mol Sci Article Nurr1 and brain-derived neurotrophic factor (BDNF) play major roles in cognition. Nurr1 regulates BDNF in midbrain dopaminergic neurons and cerebellar granule cells. Nurr1 and BDNF are also highly expressed in the cerebral cortex, a brain area important in cognition. Due to Nurr1 and BDNF tissue specificity, the regulatory effect of Nurr1 on BDNF in different brain areas cannot be generalized. The relationship between Nurr1 and BDNF in the cortex has not been investigated previously. Therefore, we examined Nurr1-mediated BDNF regulation in cortical neurons in activity-dependent and activity-independent states. Mouse primary cortical neurons were treated with the Nurr1 agonist, amodiaquine (AQ). Membrane depolarization was induced by KCl or veratridine and reversed by nimodipine. AQ and membrane depolarization significantly increased Nurr1 (p < 0.001) and BDNF (p(AQ) < 0.001, p(KCl) < 0.01) as assessed by real-time qRT-PCR. However, Nurr1 knockdown did not affect BDNF gene expression in resting or depolarized neurons. Accordingly, the positive correlation between Nurr1 and BDNF expression in AQ and membrane depolarization experiments does not imply co-regulation because Nurr1 knockdown did not affect BDNF gene expression in resting or depolarized cortical neurons. Therefore, in contrast to midbrain dopaminergic neurons and cerebellar granule cells, Nurr1 does not regulate BDNF in cortical neurons. MDPI 2022-06-20 /pmc/articles/PMC9224520/ /pubmed/35743300 http://dx.doi.org/10.3390/ijms23126853 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abdollahi, Mona
Fahnestock, Margaret
Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons
title Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons
title_full Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons
title_fullStr Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons
title_full_unstemmed Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons
title_short Nurr1 Is Not an Essential Regulator of BDNF in Mouse Cortical Neurons
title_sort nurr1 is not an essential regulator of bdnf in mouse cortical neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224520/
https://www.ncbi.nlm.nih.gov/pubmed/35743300
http://dx.doi.org/10.3390/ijms23126853
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