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Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells
With aging, sarcopenia and the associated locomotor disorders, have become serious problems. The roots of maca contain active ingredients (triterpenes) that have a preventive effect on sarcopenia. However, the effect of maca on muscle hypertrophy has not yet been investigated. The aim of this study...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224786/ https://www.ncbi.nlm.nih.gov/pubmed/35743270 http://dx.doi.org/10.3390/ijms23126825 |
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author | Yi, Dong Yoshikawa, Maki Sugimoto, Takeshi Tomoo, Keigo Okada, Yoko Hashimoto, Takeshi |
author_facet | Yi, Dong Yoshikawa, Maki Sugimoto, Takeshi Tomoo, Keigo Okada, Yoko Hashimoto, Takeshi |
author_sort | Yi, Dong |
collection | PubMed |
description | With aging, sarcopenia and the associated locomotor disorders, have become serious problems. The roots of maca contain active ingredients (triterpenes) that have a preventive effect on sarcopenia. However, the effect of maca on muscle hypertrophy has not yet been investigated. The aim of this study was to examine the effects and mechanism of maca on muscle hypertrophy by adding different concentrations of yellow maca (0.1 mg/mL and 0.2 mg/mL) to C2C12 skeletal muscle cell culture. Two days after differentiation, maca was added for two days of incubation. The muscle diameter, area, differentiation index, and multinucleation, were assessed by immunostaining, and the expression levels of the proteins related to muscle protein synthesis/degradation were examined by Western blotting. Compared with the control group, the muscle diameter and area of the myotubes in the maca groups were significantly increased, and the cell differentiation index and multinucleation were significantly higher in the maca groups. Phosphorylation of Akt and mTOR was elevated in the maca groups. Maca also promoted the phosphorylation of AMPK. These results suggest that maca may promote muscle hypertrophy, differentiation, and maturation, potentially via the muscle hypertrophic signaling pathways such as Akt and mTOR, while exploring other pathways are needed. |
format | Online Article Text |
id | pubmed-9224786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92247862022-06-24 Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells Yi, Dong Yoshikawa, Maki Sugimoto, Takeshi Tomoo, Keigo Okada, Yoko Hashimoto, Takeshi Int J Mol Sci Article With aging, sarcopenia and the associated locomotor disorders, have become serious problems. The roots of maca contain active ingredients (triterpenes) that have a preventive effect on sarcopenia. However, the effect of maca on muscle hypertrophy has not yet been investigated. The aim of this study was to examine the effects and mechanism of maca on muscle hypertrophy by adding different concentrations of yellow maca (0.1 mg/mL and 0.2 mg/mL) to C2C12 skeletal muscle cell culture. Two days after differentiation, maca was added for two days of incubation. The muscle diameter, area, differentiation index, and multinucleation, were assessed by immunostaining, and the expression levels of the proteins related to muscle protein synthesis/degradation were examined by Western blotting. Compared with the control group, the muscle diameter and area of the myotubes in the maca groups were significantly increased, and the cell differentiation index and multinucleation were significantly higher in the maca groups. Phosphorylation of Akt and mTOR was elevated in the maca groups. Maca also promoted the phosphorylation of AMPK. These results suggest that maca may promote muscle hypertrophy, differentiation, and maturation, potentially via the muscle hypertrophic signaling pathways such as Akt and mTOR, while exploring other pathways are needed. MDPI 2022-06-19 /pmc/articles/PMC9224786/ /pubmed/35743270 http://dx.doi.org/10.3390/ijms23126825 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yi, Dong Yoshikawa, Maki Sugimoto, Takeshi Tomoo, Keigo Okada, Yoko Hashimoto, Takeshi Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells |
title | Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells |
title_full | Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells |
title_fullStr | Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells |
title_full_unstemmed | Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells |
title_short | Effects of Maca on Muscle Hypertrophy in C2C12 Skeletal Muscle Cells |
title_sort | effects of maca on muscle hypertrophy in c2c12 skeletal muscle cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224786/ https://www.ncbi.nlm.nih.gov/pubmed/35743270 http://dx.doi.org/10.3390/ijms23126825 |
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