Cargando…
Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications
Microalgae peptides have many medical and industrial applications due to their functional properties. However, the rapid degradation of peptides not naturally present in biological samples represents a challenge. A strategy to increase microalgae peptide stability in biological samples is to use car...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224969/ https://www.ncbi.nlm.nih.gov/pubmed/35743862 http://dx.doi.org/10.3390/life12060831 |
_version_ | 1784733501902290944 |
---|---|
author | Torres-Díaz, Marielys Abreu-Takemura, Caren Díaz-Vázquez, Liz M. |
author_facet | Torres-Díaz, Marielys Abreu-Takemura, Caren Díaz-Vázquez, Liz M. |
author_sort | Torres-Díaz, Marielys |
collection | PubMed |
description | Microalgae peptides have many medical and industrial applications due to their functional properties. However, the rapid degradation of peptides not naturally present in biological samples represents a challenge. A strategy to increase microalgae peptide stability in biological samples is to use carriers to protect the active peptide and regulate its release. This study explores the use of gold nanoparticles (AuNPs) as carriers of the Chlorella microalgae peptide (VECYGPNRPQF). The potential of these peptide biomolecules as stabilizing agents to improve the colloidal stability of AuNPs in physiological environments is also discussed. Spectroscopic (UV-VIS, DLS) and Microscopic (TEM) analyses confirmed that the employed modification method produced spherical AuNPs by an average 15 nm diameter. Successful peptide capping of AuNPs was confirmed with TEM images and FTIR spectroscopy. The stability of the microalgae peptide increased when immobilized into the AuNPs surface, as confirmed by the observed thermal shifts in DSC and high zeta-potential values in the colloidal solution. By optimizing the synthesis of AuNPs and tracking the conferred chemical properties as AuNPs were modified with the peptide via various alternative methods, the synthesis of an effective peptide-based coating system for AuNPs and drug carriers was achieved. The microalgae peptide AuNPs showed lower ecotoxicity and better viability than the regular AuNPs. |
format | Online Article Text |
id | pubmed-9224969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92249692022-06-24 Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications Torres-Díaz, Marielys Abreu-Takemura, Caren Díaz-Vázquez, Liz M. Life (Basel) Article Microalgae peptides have many medical and industrial applications due to their functional properties. However, the rapid degradation of peptides not naturally present in biological samples represents a challenge. A strategy to increase microalgae peptide stability in biological samples is to use carriers to protect the active peptide and regulate its release. This study explores the use of gold nanoparticles (AuNPs) as carriers of the Chlorella microalgae peptide (VECYGPNRPQF). The potential of these peptide biomolecules as stabilizing agents to improve the colloidal stability of AuNPs in physiological environments is also discussed. Spectroscopic (UV-VIS, DLS) and Microscopic (TEM) analyses confirmed that the employed modification method produced spherical AuNPs by an average 15 nm diameter. Successful peptide capping of AuNPs was confirmed with TEM images and FTIR spectroscopy. The stability of the microalgae peptide increased when immobilized into the AuNPs surface, as confirmed by the observed thermal shifts in DSC and high zeta-potential values in the colloidal solution. By optimizing the synthesis of AuNPs and tracking the conferred chemical properties as AuNPs were modified with the peptide via various alternative methods, the synthesis of an effective peptide-based coating system for AuNPs and drug carriers was achieved. The microalgae peptide AuNPs showed lower ecotoxicity and better viability than the regular AuNPs. MDPI 2022-06-02 /pmc/articles/PMC9224969/ /pubmed/35743862 http://dx.doi.org/10.3390/life12060831 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Torres-Díaz, Marielys Abreu-Takemura, Caren Díaz-Vázquez, Liz M. Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications |
title | Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications |
title_full | Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications |
title_fullStr | Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications |
title_full_unstemmed | Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications |
title_short | Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications |
title_sort | microalgae peptide-stabilized gold nanoparticles as a versatile material for biomedical applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224969/ https://www.ncbi.nlm.nih.gov/pubmed/35743862 http://dx.doi.org/10.3390/life12060831 |
work_keys_str_mv | AT torresdiazmarielys microalgaepeptidestabilizedgoldnanoparticlesasaversatilematerialforbiomedicalapplications AT abreutakemuracaren microalgaepeptidestabilizedgoldnanoparticlesasaversatilematerialforbiomedicalapplications AT diazvazquezlizm microalgaepeptidestabilizedgoldnanoparticlesasaversatilematerialforbiomedicalapplications |