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Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging
Ultraviolet (UV) fluorescence is a valuable tool for the imaging of a wide range of subjects. Like all imaging techniques, the key to success depends on the correct choice of equipment and approach used. In fluorescence photography, a filter is placed in front of the camera lens to block unwanted sh...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224984/ https://www.ncbi.nlm.nih.gov/pubmed/35735961 http://dx.doi.org/10.3390/jimaging8060162 |
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author | Crowther, Jonathan |
author_facet | Crowther, Jonathan |
author_sort | Crowther, Jonathan |
collection | PubMed |
description | Ultraviolet (UV) fluorescence is a valuable tool for the imaging of a wide range of subjects. Like all imaging techniques, the key to success depends on the correct choice of equipment and approach used. In fluorescence photography, a filter is placed in front of the camera lens to block unwanted short-wavelength light from entering the camera, which would compromise the image. However, some filters exhibit fluorescence under UV light and can therefore have the potential to produce a color cast on the image. Filters also vary in how well they block unwanted light. A range of commonly used optical filters was assessed for fluorescence under UV light, and their optical transmission between 250 nm and 800 nm was measured. Finally, a simple method to enable the researcher to determine the fluorescence of the filters that they are using or wish to use for their work is described. The results indicate that the filters tested demonstrated a wide range of fluorescence under UV light and varying degrees of UV blocking. Some filters tested had equivalent or reduced fluorescence compared to Schott KV-418, which is a widely used, but, unfortunately, no longer manufactured UV blocking filter commonly used for fluorescence photography. |
format | Online Article Text |
id | pubmed-9224984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92249842022-06-24 Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging Crowther, Jonathan J Imaging Article Ultraviolet (UV) fluorescence is a valuable tool for the imaging of a wide range of subjects. Like all imaging techniques, the key to success depends on the correct choice of equipment and approach used. In fluorescence photography, a filter is placed in front of the camera lens to block unwanted short-wavelength light from entering the camera, which would compromise the image. However, some filters exhibit fluorescence under UV light and can therefore have the potential to produce a color cast on the image. Filters also vary in how well they block unwanted light. A range of commonly used optical filters was assessed for fluorescence under UV light, and their optical transmission between 250 nm and 800 nm was measured. Finally, a simple method to enable the researcher to determine the fluorescence of the filters that they are using or wish to use for their work is described. The results indicate that the filters tested demonstrated a wide range of fluorescence under UV light and varying degrees of UV blocking. Some filters tested had equivalent or reduced fluorescence compared to Schott KV-418, which is a widely used, but, unfortunately, no longer manufactured UV blocking filter commonly used for fluorescence photography. MDPI 2022-06-07 /pmc/articles/PMC9224984/ /pubmed/35735961 http://dx.doi.org/10.3390/jimaging8060162 Text en © 2022 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Crowther, Jonathan Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging |
title | Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging |
title_full | Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging |
title_fullStr | Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging |
title_full_unstemmed | Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging |
title_short | Ultraviolet Fluorescence Photography—Choosing the Correct Filters for Imaging |
title_sort | ultraviolet fluorescence photography—choosing the correct filters for imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224984/ https://www.ncbi.nlm.nih.gov/pubmed/35735961 http://dx.doi.org/10.3390/jimaging8060162 |
work_keys_str_mv | AT crowtherjonathan ultravioletfluorescencephotographychoosingthecorrectfiltersforimaging |