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Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity

The role of autophagy in lung cancer cells exposed to waterpipe smoke (WPS) is not known. Because of the important role of autophagy in tumor resistance and progression, we investigated its relationship with WP smoking. We first showed that WPS activated autophagy, as reflected by LC3 processing, in...

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Autores principales: Zaarour, Rania Faouzi, Sharda, Mohak, Azakir, Bilal, Hassan Venkatesh, Goutham, Abou Khouzam, Raefa, Rifath, Ayesha, Nizami, Zohra Nausheen, Abdullah, Fatima, Mohammad, Fatin, Karaali, Hajar, Nawafleh, Husam, Elsayed, Yehya, Chouaib, Salem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225041/
https://www.ncbi.nlm.nih.gov/pubmed/35743294
http://dx.doi.org/10.3390/ijms23126848
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author Zaarour, Rania Faouzi
Sharda, Mohak
Azakir, Bilal
Hassan Venkatesh, Goutham
Abou Khouzam, Raefa
Rifath, Ayesha
Nizami, Zohra Nausheen
Abdullah, Fatima
Mohammad, Fatin
Karaali, Hajar
Nawafleh, Husam
Elsayed, Yehya
Chouaib, Salem
author_facet Zaarour, Rania Faouzi
Sharda, Mohak
Azakir, Bilal
Hassan Venkatesh, Goutham
Abou Khouzam, Raefa
Rifath, Ayesha
Nizami, Zohra Nausheen
Abdullah, Fatima
Mohammad, Fatin
Karaali, Hajar
Nawafleh, Husam
Elsayed, Yehya
Chouaib, Salem
author_sort Zaarour, Rania Faouzi
collection PubMed
description The role of autophagy in lung cancer cells exposed to waterpipe smoke (WPS) is not known. Because of the important role of autophagy in tumor resistance and progression, we investigated its relationship with WP smoking. We first showed that WPS activated autophagy, as reflected by LC3 processing, in lung cancer cell lines. The autophagy response in smokers with lung adenocarcinoma, as compared to non-smokers with lung adenocarcinoma, was investigated further using the TCGA lung adenocarcinoma bulk RNA-seq dataset with the available patient metadata on smoking status. The results, based on a machine learning classification model using Random Forest, indicate that smokers have an increase in autophagy-activating genes. Comparative analysis of lung adenocarcinoma molecular signatures in affected patients with a long-term active exposure to smoke compared to non-smoker patients indicates a higher tumor mutational burden, a higher CD8+ T-cell level and a lower dysfunction level in smokers. While the expression of the checkpoint genes tested—PD-1, PD-L1, PD-L2 and CTLA-4—remains unchanged between smokers and non-smokers, B7-1, B7-2, IDO1 and CD200R1 were found to be higher in non-smokers than smokers. Because multiple factors in the tumor microenvironment dictate the success of immunotherapy, in addition to the expression of immune checkpoint genes, our analysis explains why patients who are smokers with lung adenocarcinoma respond better to immunotherapy, even though there are no relative differences in immune checkpoint genes in the two groups. Therefore, targeting autophagy in lung adenocarcinoma patients, in combination with checkpoint inhibitor-targeted therapies or chemotherapy, should be considered in smoker patients with lung adenocarcinoma.
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spelling pubmed-92250412022-06-24 Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity Zaarour, Rania Faouzi Sharda, Mohak Azakir, Bilal Hassan Venkatesh, Goutham Abou Khouzam, Raefa Rifath, Ayesha Nizami, Zohra Nausheen Abdullah, Fatima Mohammad, Fatin Karaali, Hajar Nawafleh, Husam Elsayed, Yehya Chouaib, Salem Int J Mol Sci Article The role of autophagy in lung cancer cells exposed to waterpipe smoke (WPS) is not known. Because of the important role of autophagy in tumor resistance and progression, we investigated its relationship with WP smoking. We first showed that WPS activated autophagy, as reflected by LC3 processing, in lung cancer cell lines. The autophagy response in smokers with lung adenocarcinoma, as compared to non-smokers with lung adenocarcinoma, was investigated further using the TCGA lung adenocarcinoma bulk RNA-seq dataset with the available patient metadata on smoking status. The results, based on a machine learning classification model using Random Forest, indicate that smokers have an increase in autophagy-activating genes. Comparative analysis of lung adenocarcinoma molecular signatures in affected patients with a long-term active exposure to smoke compared to non-smoker patients indicates a higher tumor mutational burden, a higher CD8+ T-cell level and a lower dysfunction level in smokers. While the expression of the checkpoint genes tested—PD-1, PD-L1, PD-L2 and CTLA-4—remains unchanged between smokers and non-smokers, B7-1, B7-2, IDO1 and CD200R1 were found to be higher in non-smokers than smokers. Because multiple factors in the tumor microenvironment dictate the success of immunotherapy, in addition to the expression of immune checkpoint genes, our analysis explains why patients who are smokers with lung adenocarcinoma respond better to immunotherapy, even though there are no relative differences in immune checkpoint genes in the two groups. Therefore, targeting autophagy in lung adenocarcinoma patients, in combination with checkpoint inhibitor-targeted therapies or chemotherapy, should be considered in smoker patients with lung adenocarcinoma. MDPI 2022-06-20 /pmc/articles/PMC9225041/ /pubmed/35743294 http://dx.doi.org/10.3390/ijms23126848 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zaarour, Rania Faouzi
Sharda, Mohak
Azakir, Bilal
Hassan Venkatesh, Goutham
Abou Khouzam, Raefa
Rifath, Ayesha
Nizami, Zohra Nausheen
Abdullah, Fatima
Mohammad, Fatin
Karaali, Hajar
Nawafleh, Husam
Elsayed, Yehya
Chouaib, Salem
Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity
title Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity
title_full Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity
title_fullStr Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity
title_full_unstemmed Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity
title_short Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity
title_sort genomic analysis of waterpipe smoke-induced lung tumor autophagy and plasticity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225041/
https://www.ncbi.nlm.nih.gov/pubmed/35743294
http://dx.doi.org/10.3390/ijms23126848
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