Cargando…

Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection

The pathogenicity of many bacterial strains is determined by the acquisition of virulence genes and depends on many factors. The aim of this study was to analyse the phylogenetic background, virulence patterns, and drug susceptibility of 132 E. coli isolates tested in the context of the ExPEC (Extra...

Descripción completa

Detalles Bibliográficos
Autores principales: Sarowska, Jolanta, Olszak, Tomasz, Jama-Kmiecik, Agnieszka, Frej-Madrzak, Magdalena, Futoma-Koloch, Bozena, Gawel, Andrzej, Drulis-Kawa, Zuzanna, Choroszy-Krol, Irena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225339/
https://www.ncbi.nlm.nih.gov/pubmed/35743876
http://dx.doi.org/10.3390/life12060845
_version_ 1784733594225213440
author Sarowska, Jolanta
Olszak, Tomasz
Jama-Kmiecik, Agnieszka
Frej-Madrzak, Magdalena
Futoma-Koloch, Bozena
Gawel, Andrzej
Drulis-Kawa, Zuzanna
Choroszy-Krol, Irena
author_facet Sarowska, Jolanta
Olszak, Tomasz
Jama-Kmiecik, Agnieszka
Frej-Madrzak, Magdalena
Futoma-Koloch, Bozena
Gawel, Andrzej
Drulis-Kawa, Zuzanna
Choroszy-Krol, Irena
author_sort Sarowska, Jolanta
collection PubMed
description The pathogenicity of many bacterial strains is determined by the acquisition of virulence genes and depends on many factors. The aim of this study was to analyse the phylogenetic background, virulence patterns, and drug susceptibility of 132 E. coli isolates tested in the context of the ExPEC (Extraintestinal Pathogenic E. coli) pathotype and the correlation of these features with bacterial isolation source: food (retail meat), poultry farms (AFEC—Avian Faecal E. coli), and patients with UTI (urinary tract infection) symptoms. The drug-susceptibility results of tested E. coli isolates obtained indicate that the resistance profile—ampicillin/tetracycline/trimethoprim+sulfamethoxazole/ciprofloxacin (AMP/TE/SXT/CIP)—was most frequently observed. The multidrug resistance (MDR) phenotype was found in 31.8% of isolates from poultry farms, 36.8% of strains isolated from food, and 20% of clinical samples. The greatest similarity of virulence profiles applied to isolates derived from poultry farms and food. Most of the AFEC from poultry farms and food-derived isolates belonged to commensals from phylogroups A and B1, while among the isolates from patients with UTI symptoms, the most common was the B2 phylogroup. The collective analysis showed similarity of the three studied groups of E. coli isolates in terms of the presented patterns of antimicrobial resistance, while the virulence profiles of the isolates studied showed great diversity. The phylogroup analysis showed no similarity between the poultry/food isolates and the UTI isolates, which had significant pathogenic potential.
format Online
Article
Text
id pubmed-9225339
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92253392022-06-24 Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection Sarowska, Jolanta Olszak, Tomasz Jama-Kmiecik, Agnieszka Frej-Madrzak, Magdalena Futoma-Koloch, Bozena Gawel, Andrzej Drulis-Kawa, Zuzanna Choroszy-Krol, Irena Life (Basel) Article The pathogenicity of many bacterial strains is determined by the acquisition of virulence genes and depends on many factors. The aim of this study was to analyse the phylogenetic background, virulence patterns, and drug susceptibility of 132 E. coli isolates tested in the context of the ExPEC (Extraintestinal Pathogenic E. coli) pathotype and the correlation of these features with bacterial isolation source: food (retail meat), poultry farms (AFEC—Avian Faecal E. coli), and patients with UTI (urinary tract infection) symptoms. The drug-susceptibility results of tested E. coli isolates obtained indicate that the resistance profile—ampicillin/tetracycline/trimethoprim+sulfamethoxazole/ciprofloxacin (AMP/TE/SXT/CIP)—was most frequently observed. The multidrug resistance (MDR) phenotype was found in 31.8% of isolates from poultry farms, 36.8% of strains isolated from food, and 20% of clinical samples. The greatest similarity of virulence profiles applied to isolates derived from poultry farms and food. Most of the AFEC from poultry farms and food-derived isolates belonged to commensals from phylogroups A and B1, while among the isolates from patients with UTI symptoms, the most common was the B2 phylogroup. The collective analysis showed similarity of the three studied groups of E. coli isolates in terms of the presented patterns of antimicrobial resistance, while the virulence profiles of the isolates studied showed great diversity. The phylogroup analysis showed no similarity between the poultry/food isolates and the UTI isolates, which had significant pathogenic potential. MDPI 2022-06-06 /pmc/articles/PMC9225339/ /pubmed/35743876 http://dx.doi.org/10.3390/life12060845 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sarowska, Jolanta
Olszak, Tomasz
Jama-Kmiecik, Agnieszka
Frej-Madrzak, Magdalena
Futoma-Koloch, Bozena
Gawel, Andrzej
Drulis-Kawa, Zuzanna
Choroszy-Krol, Irena
Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection
title Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection
title_full Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection
title_fullStr Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection
title_full_unstemmed Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection
title_short Comparative Characteristics and Pathogenic Potential of Escherichia coli Isolates Originating from Poultry Farms, Retail Meat, and Human Urinary Tract Infection
title_sort comparative characteristics and pathogenic potential of escherichia coli isolates originating from poultry farms, retail meat, and human urinary tract infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225339/
https://www.ncbi.nlm.nih.gov/pubmed/35743876
http://dx.doi.org/10.3390/life12060845
work_keys_str_mv AT sarowskajolanta comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection
AT olszaktomasz comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection
AT jamakmiecikagnieszka comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection
AT frejmadrzakmagdalena comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection
AT futomakolochbozena comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection
AT gawelandrzej comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection
AT druliskawazuzanna comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection
AT choroszykrolirena comparativecharacteristicsandpathogenicpotentialofescherichiacoliisolatesoriginatingfrompoultryfarmsretailmeatandhumanurinarytractinfection