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Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies
Microorganisms have evolved various life-history strategies to survive fluctuating resource conditions in soils. However, it remains elusive how the life-history strategies of microorganisms influence their processing of organic carbon, which may affect microbial interactions and carbon cycling in s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225577/ https://www.ncbi.nlm.nih.gov/pubmed/35756023 http://dx.doi.org/10.3389/fmicb.2022.914472 |
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author | Wang, Ying Wilhelm, Roland C. Swenson, Tami L. Silver, Anita Andeer, Peter F. Golini, Amber Kosina, Suzanne M. Bowen, Benjamin P. Buckley, Daniel H. Northen, Trent R. |
author_facet | Wang, Ying Wilhelm, Roland C. Swenson, Tami L. Silver, Anita Andeer, Peter F. Golini, Amber Kosina, Suzanne M. Bowen, Benjamin P. Buckley, Daniel H. Northen, Trent R. |
author_sort | Wang, Ying |
collection | PubMed |
description | Microorganisms have evolved various life-history strategies to survive fluctuating resource conditions in soils. However, it remains elusive how the life-history strategies of microorganisms influence their processing of organic carbon, which may affect microbial interactions and carbon cycling in soils. Here, we characterized the genomic traits, exometabolite profiles, and interactions of soil bacteria representing copiotrophic and oligotrophic strategists. Isolates were selected based on differences in ribosomal RNA operon (rrn) copy number, as a proxy for life-history strategies, with pairs of “high” and “low” rrn copy number isolates represented within the Micrococcales, Corynebacteriales, and Bacillales. We found that high rrn isolates consumed a greater diversity and amount of substrates than low rrn isolates in a defined growth medium containing common soil metabolites. We estimated overlap in substrate utilization profiles to predict the potential for resource competition and found that high rrn isolates tended to have a greater potential for competitive interactions. The predicted interactions positively correlated with the measured interactions that were dominated by negative interactions as determined through sequential growth experiments. This suggests that resource competition was a major force governing interactions among isolates, while cross-feeding of metabolic secretion likely contributed to the relatively rare positive interactions observed. By connecting bacterial life-history strategies, genomic features, and metabolism, our study advances the understanding of the links between bacterial community composition and the transformation of carbon in soils. |
format | Online Article Text |
id | pubmed-9225577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92255772022-06-24 Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies Wang, Ying Wilhelm, Roland C. Swenson, Tami L. Silver, Anita Andeer, Peter F. Golini, Amber Kosina, Suzanne M. Bowen, Benjamin P. Buckley, Daniel H. Northen, Trent R. Front Microbiol Microbiology Microorganisms have evolved various life-history strategies to survive fluctuating resource conditions in soils. However, it remains elusive how the life-history strategies of microorganisms influence their processing of organic carbon, which may affect microbial interactions and carbon cycling in soils. Here, we characterized the genomic traits, exometabolite profiles, and interactions of soil bacteria representing copiotrophic and oligotrophic strategists. Isolates were selected based on differences in ribosomal RNA operon (rrn) copy number, as a proxy for life-history strategies, with pairs of “high” and “low” rrn copy number isolates represented within the Micrococcales, Corynebacteriales, and Bacillales. We found that high rrn isolates consumed a greater diversity and amount of substrates than low rrn isolates in a defined growth medium containing common soil metabolites. We estimated overlap in substrate utilization profiles to predict the potential for resource competition and found that high rrn isolates tended to have a greater potential for competitive interactions. The predicted interactions positively correlated with the measured interactions that were dominated by negative interactions as determined through sequential growth experiments. This suggests that resource competition was a major force governing interactions among isolates, while cross-feeding of metabolic secretion likely contributed to the relatively rare positive interactions observed. By connecting bacterial life-history strategies, genomic features, and metabolism, our study advances the understanding of the links between bacterial community composition and the transformation of carbon in soils. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9225577/ /pubmed/35756023 http://dx.doi.org/10.3389/fmicb.2022.914472 Text en Copyright © 2022 Wang, Wilhelm, Swenson, Silver, Andeer, Golini, Kosina, Bowen, Buckley and Northen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Ying Wilhelm, Roland C. Swenson, Tami L. Silver, Anita Andeer, Peter F. Golini, Amber Kosina, Suzanne M. Bowen, Benjamin P. Buckley, Daniel H. Northen, Trent R. Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies |
title | Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies |
title_full | Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies |
title_fullStr | Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies |
title_full_unstemmed | Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies |
title_short | Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies |
title_sort | substrate utilization and competitive interactions among soil bacteria vary with life-history strategies |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225577/ https://www.ncbi.nlm.nih.gov/pubmed/35756023 http://dx.doi.org/10.3389/fmicb.2022.914472 |
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