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Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies

Microorganisms have evolved various life-history strategies to survive fluctuating resource conditions in soils. However, it remains elusive how the life-history strategies of microorganisms influence their processing of organic carbon, which may affect microbial interactions and carbon cycling in s...

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Autores principales: Wang, Ying, Wilhelm, Roland C., Swenson, Tami L., Silver, Anita, Andeer, Peter F., Golini, Amber, Kosina, Suzanne M., Bowen, Benjamin P., Buckley, Daniel H., Northen, Trent R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225577/
https://www.ncbi.nlm.nih.gov/pubmed/35756023
http://dx.doi.org/10.3389/fmicb.2022.914472
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author Wang, Ying
Wilhelm, Roland C.
Swenson, Tami L.
Silver, Anita
Andeer, Peter F.
Golini, Amber
Kosina, Suzanne M.
Bowen, Benjamin P.
Buckley, Daniel H.
Northen, Trent R.
author_facet Wang, Ying
Wilhelm, Roland C.
Swenson, Tami L.
Silver, Anita
Andeer, Peter F.
Golini, Amber
Kosina, Suzanne M.
Bowen, Benjamin P.
Buckley, Daniel H.
Northen, Trent R.
author_sort Wang, Ying
collection PubMed
description Microorganisms have evolved various life-history strategies to survive fluctuating resource conditions in soils. However, it remains elusive how the life-history strategies of microorganisms influence their processing of organic carbon, which may affect microbial interactions and carbon cycling in soils. Here, we characterized the genomic traits, exometabolite profiles, and interactions of soil bacteria representing copiotrophic and oligotrophic strategists. Isolates were selected based on differences in ribosomal RNA operon (rrn) copy number, as a proxy for life-history strategies, with pairs of “high” and “low” rrn copy number isolates represented within the Micrococcales, Corynebacteriales, and Bacillales. We found that high rrn isolates consumed a greater diversity and amount of substrates than low rrn isolates in a defined growth medium containing common soil metabolites. We estimated overlap in substrate utilization profiles to predict the potential for resource competition and found that high rrn isolates tended to have a greater potential for competitive interactions. The predicted interactions positively correlated with the measured interactions that were dominated by negative interactions as determined through sequential growth experiments. This suggests that resource competition was a major force governing interactions among isolates, while cross-feeding of metabolic secretion likely contributed to the relatively rare positive interactions observed. By connecting bacterial life-history strategies, genomic features, and metabolism, our study advances the understanding of the links between bacterial community composition and the transformation of carbon in soils.
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spelling pubmed-92255772022-06-24 Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies Wang, Ying Wilhelm, Roland C. Swenson, Tami L. Silver, Anita Andeer, Peter F. Golini, Amber Kosina, Suzanne M. Bowen, Benjamin P. Buckley, Daniel H. Northen, Trent R. Front Microbiol Microbiology Microorganisms have evolved various life-history strategies to survive fluctuating resource conditions in soils. However, it remains elusive how the life-history strategies of microorganisms influence their processing of organic carbon, which may affect microbial interactions and carbon cycling in soils. Here, we characterized the genomic traits, exometabolite profiles, and interactions of soil bacteria representing copiotrophic and oligotrophic strategists. Isolates were selected based on differences in ribosomal RNA operon (rrn) copy number, as a proxy for life-history strategies, with pairs of “high” and “low” rrn copy number isolates represented within the Micrococcales, Corynebacteriales, and Bacillales. We found that high rrn isolates consumed a greater diversity and amount of substrates than low rrn isolates in a defined growth medium containing common soil metabolites. We estimated overlap in substrate utilization profiles to predict the potential for resource competition and found that high rrn isolates tended to have a greater potential for competitive interactions. The predicted interactions positively correlated with the measured interactions that were dominated by negative interactions as determined through sequential growth experiments. This suggests that resource competition was a major force governing interactions among isolates, while cross-feeding of metabolic secretion likely contributed to the relatively rare positive interactions observed. By connecting bacterial life-history strategies, genomic features, and metabolism, our study advances the understanding of the links between bacterial community composition and the transformation of carbon in soils. Frontiers Media S.A. 2022-06-09 /pmc/articles/PMC9225577/ /pubmed/35756023 http://dx.doi.org/10.3389/fmicb.2022.914472 Text en Copyright © 2022 Wang, Wilhelm, Swenson, Silver, Andeer, Golini, Kosina, Bowen, Buckley and Northen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Ying
Wilhelm, Roland C.
Swenson, Tami L.
Silver, Anita
Andeer, Peter F.
Golini, Amber
Kosina, Suzanne M.
Bowen, Benjamin P.
Buckley, Daniel H.
Northen, Trent R.
Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies
title Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies
title_full Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies
title_fullStr Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies
title_full_unstemmed Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies
title_short Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies
title_sort substrate utilization and competitive interactions among soil bacteria vary with life-history strategies
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225577/
https://www.ncbi.nlm.nih.gov/pubmed/35756023
http://dx.doi.org/10.3389/fmicb.2022.914472
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