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Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus

Platycodon grandiflorus, a perennial flowering plant widely distributed in China and South Korea, is an excellent resource for both food and medicine. The main active compounds of P. grandiflorus are triterpenoid saponins. WRKY transcription factors (TFs) are among the largest gene families in plant...

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Autores principales: Li, Jing, Yu, Hanwen, Liu, Mengli, Chen, Bowen, Dong, Nan, Chang, Xiangwei, Wang, Jutao, Xing, Shihai, Peng, Huasheng, Zha, Liangping, Gui, Shuangying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225661/
https://www.ncbi.nlm.nih.gov/pubmed/35730590
http://dx.doi.org/10.1080/15592324.2022.2089473
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author Li, Jing
Yu, Hanwen
Liu, Mengli
Chen, Bowen
Dong, Nan
Chang, Xiangwei
Wang, Jutao
Xing, Shihai
Peng, Huasheng
Zha, Liangping
Gui, Shuangying
author_facet Li, Jing
Yu, Hanwen
Liu, Mengli
Chen, Bowen
Dong, Nan
Chang, Xiangwei
Wang, Jutao
Xing, Shihai
Peng, Huasheng
Zha, Liangping
Gui, Shuangying
author_sort Li, Jing
collection PubMed
description Platycodon grandiflorus, a perennial flowering plant widely distributed in China and South Korea, is an excellent resource for both food and medicine. The main active compounds of P. grandiflorus are triterpenoid saponins. WRKY transcription factors (TFs) are among the largest gene families in plants and play an important role in regulating plant terpenoid accumulation, physiological metabolism, and stress response. Numerous studies have been reported on other medicinal plants; however, little is known about WRKY genes in P. grandiflorus. In this study, 27 PgWRKYs were identified in the P. grandiflorus transcriptome. Phylogenetic analysis showed that PgWRKY genes were clustered into three main groups and five subgroups. Transcriptome analysis showed that the PgWRKY gene expression patterns in different tissues differed between those in Tongcheng City (Southern Anhui) and Taihe County (Northern Anhui). Gene expression analysis based on RNA sequencing and qRT-PCR analysis showed that most PgWRKY genes were expressed after induction with methyl jasmonate (MeJA). Co-expressing PgWRKY genes with triterpenoid biosynthesis pathway genes revealed four PgWRKY genes that may have functions in triterpenoid biosynthesis. Additionally, functional annotation and protein-protein interaction analysis of PgWRKY proteins were performed to predict their roles in potential regulatory networks. Thus, we systematically analyzed the structure, evolution, and expression patterns of PgWRKY genes to provide an important theoretical basis for further exploring the molecular basis and regulatory mechanism of WRKY TFs in triterpenoid biosynthesis.
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spelling pubmed-92256612022-06-24 Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus Li, Jing Yu, Hanwen Liu, Mengli Chen, Bowen Dong, Nan Chang, Xiangwei Wang, Jutao Xing, Shihai Peng, Huasheng Zha, Liangping Gui, Shuangying Plant Signal Behav Article Platycodon grandiflorus, a perennial flowering plant widely distributed in China and South Korea, is an excellent resource for both food and medicine. The main active compounds of P. grandiflorus are triterpenoid saponins. WRKY transcription factors (TFs) are among the largest gene families in plants and play an important role in regulating plant terpenoid accumulation, physiological metabolism, and stress response. Numerous studies have been reported on other medicinal plants; however, little is known about WRKY genes in P. grandiflorus. In this study, 27 PgWRKYs were identified in the P. grandiflorus transcriptome. Phylogenetic analysis showed that PgWRKY genes were clustered into three main groups and five subgroups. Transcriptome analysis showed that the PgWRKY gene expression patterns in different tissues differed between those in Tongcheng City (Southern Anhui) and Taihe County (Northern Anhui). Gene expression analysis based on RNA sequencing and qRT-PCR analysis showed that most PgWRKY genes were expressed after induction with methyl jasmonate (MeJA). Co-expressing PgWRKY genes with triterpenoid biosynthesis pathway genes revealed four PgWRKY genes that may have functions in triterpenoid biosynthesis. Additionally, functional annotation and protein-protein interaction analysis of PgWRKY proteins were performed to predict their roles in potential regulatory networks. Thus, we systematically analyzed the structure, evolution, and expression patterns of PgWRKY genes to provide an important theoretical basis for further exploring the molecular basis and regulatory mechanism of WRKY TFs in triterpenoid biosynthesis. Taylor & Francis 2022-06-22 /pmc/articles/PMC9225661/ /pubmed/35730590 http://dx.doi.org/10.1080/15592324.2022.2089473 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Li, Jing
Yu, Hanwen
Liu, Mengli
Chen, Bowen
Dong, Nan
Chang, Xiangwei
Wang, Jutao
Xing, Shihai
Peng, Huasheng
Zha, Liangping
Gui, Shuangying
Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus
title Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus
title_full Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus
title_fullStr Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus
title_full_unstemmed Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus
title_short Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus
title_sort transcriptome-wide identification of wrky transcription factors and their expression profiles in response to methyl jasmonate in platycodon grandiflorus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225661/
https://www.ncbi.nlm.nih.gov/pubmed/35730590
http://dx.doi.org/10.1080/15592324.2022.2089473
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