Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways

Adipose mesenchymal stromal cell-derived exosomes (ADSC-Exos) have shown great potential in the treatment of oxidative stress induced by ischemia-reperfusion injury. However, alleviation of testicular torsion injury by ADSC-Exos has not been reported. Therefore, we investigated the protective effect...

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Autores principales: Liu, Hengchen, Shi, Manyu, Li, Xiangqi, Lu, Wenjun, Zhang, Mingzhao, Zhang, Tingting, Wu, Yang, Zhang, Zenan, Cui, Qingbo, Yang, Shulong, Li, Zhaozhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225846/
https://www.ncbi.nlm.nih.gov/pubmed/35757503
http://dx.doi.org/10.1155/2022/8065771
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author Liu, Hengchen
Shi, Manyu
Li, Xiangqi
Lu, Wenjun
Zhang, Mingzhao
Zhang, Tingting
Wu, Yang
Zhang, Zenan
Cui, Qingbo
Yang, Shulong
Li, Zhaozhu
author_facet Liu, Hengchen
Shi, Manyu
Li, Xiangqi
Lu, Wenjun
Zhang, Mingzhao
Zhang, Tingting
Wu, Yang
Zhang, Zenan
Cui, Qingbo
Yang, Shulong
Li, Zhaozhu
author_sort Liu, Hengchen
collection PubMed
description Adipose mesenchymal stromal cell-derived exosomes (ADSC-Exos) have shown great potential in the treatment of oxidative stress induced by ischemia-reperfusion injury. However, alleviation of testicular torsion injury by ADSC-Exos has not been reported. Therefore, we investigated the protective effect of ADSC-Exos against testicular torsion-detorsion injury. ADSC-Exos were isolated by ultracentrifugation and injected into torsion-detorsion-affected testes of rats. H&E staining and sperm quality were used to evaluate the therapeutic effects of ADSC-Exos, and tissue oxidative stress was measured by determining MDA and SOD levels. In addition, TUNEL staining and immunohistological analysis (Ki67, Cleaved Caspase-3, IL-6, IL-10, CCR7, and CD163) were used to clarify the effects of ADSC-Exos on spermatogenic cell proliferation, apoptosis, and the inflammatory microenvironment in vivo. Possible signaling pathways were predicted using sequencing technology and bioinformatics analysis. The predicted signaling pathways were validated in vitro by assessing the proliferation (EdU assay), migration (transwell assay and scratch test), and apoptosis (flow cytometry, TUNEL staining, and western blotting) of spermatogenic cells. The results showed that ADSC-Exos alleviated testicular torsion-detorsion injury by attenuating oxidative stress and the inflammatory response. In addition, ADSC-Exos promoted the proliferation and migration of spermatogenic cells and inhibited their apoptosis by activating the PI3K/AKT and MAPK/ERK1/2 signaling pathways.
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spelling pubmed-92258462022-06-24 Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways Liu, Hengchen Shi, Manyu Li, Xiangqi Lu, Wenjun Zhang, Mingzhao Zhang, Tingting Wu, Yang Zhang, Zenan Cui, Qingbo Yang, Shulong Li, Zhaozhu Oxid Med Cell Longev Research Article Adipose mesenchymal stromal cell-derived exosomes (ADSC-Exos) have shown great potential in the treatment of oxidative stress induced by ischemia-reperfusion injury. However, alleviation of testicular torsion injury by ADSC-Exos has not been reported. Therefore, we investigated the protective effect of ADSC-Exos against testicular torsion-detorsion injury. ADSC-Exos were isolated by ultracentrifugation and injected into torsion-detorsion-affected testes of rats. H&E staining and sperm quality were used to evaluate the therapeutic effects of ADSC-Exos, and tissue oxidative stress was measured by determining MDA and SOD levels. In addition, TUNEL staining and immunohistological analysis (Ki67, Cleaved Caspase-3, IL-6, IL-10, CCR7, and CD163) were used to clarify the effects of ADSC-Exos on spermatogenic cell proliferation, apoptosis, and the inflammatory microenvironment in vivo. Possible signaling pathways were predicted using sequencing technology and bioinformatics analysis. The predicted signaling pathways were validated in vitro by assessing the proliferation (EdU assay), migration (transwell assay and scratch test), and apoptosis (flow cytometry, TUNEL staining, and western blotting) of spermatogenic cells. The results showed that ADSC-Exos alleviated testicular torsion-detorsion injury by attenuating oxidative stress and the inflammatory response. In addition, ADSC-Exos promoted the proliferation and migration of spermatogenic cells and inhibited their apoptosis by activating the PI3K/AKT and MAPK/ERK1/2 signaling pathways. Hindawi 2022-06-16 /pmc/articles/PMC9225846/ /pubmed/35757503 http://dx.doi.org/10.1155/2022/8065771 Text en Copyright © 2022 Hengchen Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Hengchen
Shi, Manyu
Li, Xiangqi
Lu, Wenjun
Zhang, Mingzhao
Zhang, Tingting
Wu, Yang
Zhang, Zenan
Cui, Qingbo
Yang, Shulong
Li, Zhaozhu
Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways
title Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways
title_full Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways
title_fullStr Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways
title_full_unstemmed Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways
title_short Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 Pathways
title_sort adipose mesenchymal stromal cell-derived exosomes prevent testicular torsion injury via activating pi3k/akt and mapk/erk1/2 pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225846/
https://www.ncbi.nlm.nih.gov/pubmed/35757503
http://dx.doi.org/10.1155/2022/8065771
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