Cargando…

Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior

The interaction between the aluminum salt-based adjuvants and the antigen in the vaccine formulation is one of the determining factors affecting the immuno-potentiation effect of vaccines. However, it is not clear how the intrinsic properties of the adjuvants could affect this interaction, which lim...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Ge, Liang, Zhihui, Yu, Zilan, Li, Min, Yang, Wenqi, Zhang, Yawei, Zhao, Yuhang, Yang, Cheng, Xue, Changying, Shi, Li, Sun, Bingbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226065/
https://www.ncbi.nlm.nih.gov/pubmed/35739192
http://dx.doi.org/10.1038/s41541-022-00495-9
_version_ 1784733768506933248
author Yu, Ge
Liang, Zhihui
Yu, Zilan
Li, Min
Yang, Wenqi
Zhang, Yawei
Zhao, Yuhang
Yang, Cheng
Xue, Changying
Shi, Li
Sun, Bingbing
author_facet Yu, Ge
Liang, Zhihui
Yu, Zilan
Li, Min
Yang, Wenqi
Zhang, Yawei
Zhao, Yuhang
Yang, Cheng
Xue, Changying
Shi, Li
Sun, Bingbing
author_sort Yu, Ge
collection PubMed
description The interaction between the aluminum salt-based adjuvants and the antigen in the vaccine formulation is one of the determining factors affecting the immuno-potentiation effect of vaccines. However, it is not clear how the intrinsic properties of the adjuvants could affect this interaction, which limits to benefit the improvement of existing adjuvants and further formulation of new vaccines. Here, we engineered aluminum oxyhydroxide (AlOOH) nanorods and used a variety of antigens including hepatitis B surface antigen (HBsAg), SARS-CoV-2 spike protein receptor-binding domain (RBD), bovine serum albumin (BSA) and ovalbumin (OVA) to identify the key physicochemical properties of adjuvant that determine the antigen adsorption at the nano-bio interface between selected antigen and AlOOH nanorod adjuvant. By using various physicochemical and biophysical characterization methods, it was demonstrated that the surface hydroxyl contents of AlOOH nanorods affected the adsorptive strength of the antigen and their specific surface area determined the adsorptive capacity of the antigen. In addition, surface hydroxyl contents had an impact on the stability of the adsorbed antigen. By engineering the key intrinsic characteristics of aluminum-based adjuvants, the antigen adsorption behavior with the aluminum adjuvant could be regulated. This will facilitate the design of vaccine formulations to optimize the adsorption and stability of the antigen in vaccine.
format Online
Article
Text
id pubmed-9226065
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-92260652022-06-25 Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior Yu, Ge Liang, Zhihui Yu, Zilan Li, Min Yang, Wenqi Zhang, Yawei Zhao, Yuhang Yang, Cheng Xue, Changying Shi, Li Sun, Bingbing NPJ Vaccines Article The interaction between the aluminum salt-based adjuvants and the antigen in the vaccine formulation is one of the determining factors affecting the immuno-potentiation effect of vaccines. However, it is not clear how the intrinsic properties of the adjuvants could affect this interaction, which limits to benefit the improvement of existing adjuvants and further formulation of new vaccines. Here, we engineered aluminum oxyhydroxide (AlOOH) nanorods and used a variety of antigens including hepatitis B surface antigen (HBsAg), SARS-CoV-2 spike protein receptor-binding domain (RBD), bovine serum albumin (BSA) and ovalbumin (OVA) to identify the key physicochemical properties of adjuvant that determine the antigen adsorption at the nano-bio interface between selected antigen and AlOOH nanorod adjuvant. By using various physicochemical and biophysical characterization methods, it was demonstrated that the surface hydroxyl contents of AlOOH nanorods affected the adsorptive strength of the antigen and their specific surface area determined the adsorptive capacity of the antigen. In addition, surface hydroxyl contents had an impact on the stability of the adsorbed antigen. By engineering the key intrinsic characteristics of aluminum-based adjuvants, the antigen adsorption behavior with the aluminum adjuvant could be regulated. This will facilitate the design of vaccine formulations to optimize the adsorption and stability of the antigen in vaccine. Nature Publishing Group UK 2022-06-23 /pmc/articles/PMC9226065/ /pubmed/35739192 http://dx.doi.org/10.1038/s41541-022-00495-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yu, Ge
Liang, Zhihui
Yu, Zilan
Li, Min
Yang, Wenqi
Zhang, Yawei
Zhao, Yuhang
Yang, Cheng
Xue, Changying
Shi, Li
Sun, Bingbing
Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior
title Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior
title_full Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior
title_fullStr Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior
title_full_unstemmed Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior
title_short Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior
title_sort engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating the antigen adsorption behavior
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226065/
https://www.ncbi.nlm.nih.gov/pubmed/35739192
http://dx.doi.org/10.1038/s41541-022-00495-9
work_keys_str_mv AT yuge engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT liangzhihui engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT yuzilan engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT limin engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT yangwenqi engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT zhangyawei engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT zhaoyuhang engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT yangcheng engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT xuechangying engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT shili engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior
AT sunbingbing engineeringthehydroxylcontentonaluminumoxyhydroxidenanorodforelucidatingtheantigenadsorptionbehavior