Cargando…

Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light

Carboplatin (CP), a platinum analog, is one of the most widely used chemotherapeutic agents in the treatment of colorectal cancer. Although platinum-based drugs are quite effective in anticancer treatments, their use in a wide spectrum and effective treatment possibilities are limited due to their s...

Descripción completa

Detalles Bibliográficos
Autores principales: Danışman-Kalındemirtaş, Ferdane, Kariper, İshak Afşin, Erdemir, Gökçe, Sert, Esra, Erdem-Kuruca, Serap
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226150/
https://www.ncbi.nlm.nih.gov/pubmed/35739313
http://dx.doi.org/10.1038/s41598-022-15051-7
_version_ 1784733788460285952
author Danışman-Kalındemirtaş, Ferdane
Kariper, İshak Afşin
Erdemir, Gökçe
Sert, Esra
Erdem-Kuruca, Serap
author_facet Danışman-Kalındemirtaş, Ferdane
Kariper, İshak Afşin
Erdemir, Gökçe
Sert, Esra
Erdem-Kuruca, Serap
author_sort Danışman-Kalındemirtaş, Ferdane
collection PubMed
description Carboplatin (CP), a platinum analog, is one of the most widely used chemotherapeutic agents in the treatment of colorectal cancer. Although platinum-based drugs are quite effective in anticancer treatments, their use in a wide spectrum and effective treatment possibilities are limited due to their systemic side effects and drug resistance development. In recent years, studies have focused on increasing the therapeutic efficacy of platinum-based drugs with drug delivery systems. Gelatin, a protein, obtained by the hydrolysis of collagen, is a biocompatible and biodegradable material that can be used in nano drug delivery systems. In this study, CP-loaded gelatin-based NPs (CP-NPs) were exposed to IR light in different temperatures at 30, 35, 40, 45, and 50 °C and characterized by FESEM-EDX, FTIR, UV–Vis, DLS. Accordingly, we synthesized gelatin-based CP-NPs of different sizes between 10–290 nm by exposure to IR. We found that CP-NPs-50, 16 nm nano-sized, obtained at 50 °C had the most cytotoxicity and was 2.2 times more effective than the free drug in HCT 116 colon cancer cells. Moreover, we showed that the cytotoxicity of CP-NPs-50 in normal HUVEC cells was lower. Additionally, we demonstrated that CP-NPs enhanced apoptotic activity while not developing MDR1-related resistance in colon cancer cells. In this study, for the first time drug loaded gelatin-based nanoparticles were synthesized in different sizes with a newly self-assembly method by exposing them to infrared light at different temperatures and their anticancer effects were evaluated subsequently.
format Online
Article
Text
id pubmed-9226150
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-92261502022-06-25 Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light Danışman-Kalındemirtaş, Ferdane Kariper, İshak Afşin Erdemir, Gökçe Sert, Esra Erdem-Kuruca, Serap Sci Rep Article Carboplatin (CP), a platinum analog, is one of the most widely used chemotherapeutic agents in the treatment of colorectal cancer. Although platinum-based drugs are quite effective in anticancer treatments, their use in a wide spectrum and effective treatment possibilities are limited due to their systemic side effects and drug resistance development. In recent years, studies have focused on increasing the therapeutic efficacy of platinum-based drugs with drug delivery systems. Gelatin, a protein, obtained by the hydrolysis of collagen, is a biocompatible and biodegradable material that can be used in nano drug delivery systems. In this study, CP-loaded gelatin-based NPs (CP-NPs) were exposed to IR light in different temperatures at 30, 35, 40, 45, and 50 °C and characterized by FESEM-EDX, FTIR, UV–Vis, DLS. Accordingly, we synthesized gelatin-based CP-NPs of different sizes between 10–290 nm by exposure to IR. We found that CP-NPs-50, 16 nm nano-sized, obtained at 50 °C had the most cytotoxicity and was 2.2 times more effective than the free drug in HCT 116 colon cancer cells. Moreover, we showed that the cytotoxicity of CP-NPs-50 in normal HUVEC cells was lower. Additionally, we demonstrated that CP-NPs enhanced apoptotic activity while not developing MDR1-related resistance in colon cancer cells. In this study, for the first time drug loaded gelatin-based nanoparticles were synthesized in different sizes with a newly self-assembly method by exposing them to infrared light at different temperatures and their anticancer effects were evaluated subsequently. Nature Publishing Group UK 2022-06-23 /pmc/articles/PMC9226150/ /pubmed/35739313 http://dx.doi.org/10.1038/s41598-022-15051-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Danışman-Kalındemirtaş, Ferdane
Kariper, İshak Afşin
Erdemir, Gökçe
Sert, Esra
Erdem-Kuruca, Serap
Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
title Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
title_full Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
title_fullStr Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
title_full_unstemmed Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
title_short Evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
title_sort evaluation of anticancer effects of carboplatin–gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to ir light
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226150/
https://www.ncbi.nlm.nih.gov/pubmed/35739313
http://dx.doi.org/10.1038/s41598-022-15051-7
work_keys_str_mv AT danısmankalındemirtasferdane evaluationofanticancereffectsofcarboplatingelatinnanoparticlesindifferentsizessynthesizedwithnewlyselfassemblymethodbyexposuretoirlight
AT kariperishakafsin evaluationofanticancereffectsofcarboplatingelatinnanoparticlesindifferentsizessynthesizedwithnewlyselfassemblymethodbyexposuretoirlight
AT erdemirgokce evaluationofanticancereffectsofcarboplatingelatinnanoparticlesindifferentsizessynthesizedwithnewlyselfassemblymethodbyexposuretoirlight
AT sertesra evaluationofanticancereffectsofcarboplatingelatinnanoparticlesindifferentsizessynthesizedwithnewlyselfassemblymethodbyexposuretoirlight
AT erdemkurucaserap evaluationofanticancereffectsofcarboplatingelatinnanoparticlesindifferentsizessynthesizedwithnewlyselfassemblymethodbyexposuretoirlight