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An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations

Simultaneous targeting multiple genes is a big advantage of CRISPR (clustered regularly interspaced short palindromic repeats) genome editing but challenging to achieve in CRISPR screening. The crosstalk among genes or gene products is a common and fundamental mechanism to ensure cellular stability...

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Detalles Bibliográficos
Autores principales:	Lu, Zhike, Ni, Ke, Wang, Yingying, Zhou, Yangfan, Li, Yini, Yan, Jianfeng, Song, Qingkai, Liu, Min, Xu, Yujun, Yu, Zhenxing, Guo, Tiannan, Ma, Lijia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226518/ https://www.ncbi.nlm.nih.gov/pubmed/35670669 http://dx.doi.org/10.1093/nar/gkac458

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