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CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity

CRISPR-Cas12a (Cpf1) is a bacterial RNA-guided nuclease that cuts double-stranded DNA (dsDNA) at sites specified by a CRISPR RNA (crRNA) guide. Additional activities have been ascribed to this enzyme in vitro: site-specific (cis) single-stranded DNA (ssDNA) cleavage and indiscriminate (trans) degrad...

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Detalles Bibliográficos
Autores principales:	Marino, Nicole D, Pinilla-Redondo, Rafael, Bondy-Denomy, Joseph
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226536/ https://www.ncbi.nlm.nih.gov/pubmed/35670674 http://dx.doi.org/10.1093/nar/gkac462

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