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False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators

Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard for diagnosing coronavirus disease 2019 (COVID-19). However, RT-PCR may yield false-positive results, leading to unnecessary countermeasures. Here, we report a “positive” nucleic acid test on a 10-pooled sample d...

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Detalles Bibliográficos
Autores principales: Kong, Xiang-Qi, Wang, Yong-Jing, Fang, Zan-Xi, Yang, Tian-Ci, Tong, Man-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226675/
https://www.ncbi.nlm.nih.gov/pubmed/35755058
http://dx.doi.org/10.3389/fmed.2022.847407
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author Kong, Xiang-Qi
Wang, Yong-Jing
Fang, Zan-Xi
Yang, Tian-Ci
Tong, Man-Li
author_facet Kong, Xiang-Qi
Wang, Yong-Jing
Fang, Zan-Xi
Yang, Tian-Ci
Tong, Man-Li
author_sort Kong, Xiang-Qi
collection PubMed
description Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard for diagnosing coronavirus disease 2019 (COVID-19). However, RT-PCR may yield false-positive results, leading to unnecessary countermeasures. Here, we report a “positive” nucleic acid test on a 10-pooled sample during the routine screening that caused many adverse societal effects, and financial and resource losses. However, they were subsequently determined to be a case of vaccine contamination. This case study increases awareness of false-positive RT-PCR results for SARS-CoV-2, especially when participants are vaccinators. Moreover, it could provide relevant suggestions to prevent the recurrence of such incidents.
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spelling pubmed-92266752022-06-25 False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators Kong, Xiang-Qi Wang, Yong-Jing Fang, Zan-Xi Yang, Tian-Ci Tong, Man-Li Front Med (Lausanne) Medicine Real-time reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard for diagnosing coronavirus disease 2019 (COVID-19). However, RT-PCR may yield false-positive results, leading to unnecessary countermeasures. Here, we report a “positive” nucleic acid test on a 10-pooled sample during the routine screening that caused many adverse societal effects, and financial and resource losses. However, they were subsequently determined to be a case of vaccine contamination. This case study increases awareness of false-positive RT-PCR results for SARS-CoV-2, especially when participants are vaccinators. Moreover, it could provide relevant suggestions to prevent the recurrence of such incidents. Frontiers Media S.A. 2022-06-10 /pmc/articles/PMC9226675/ /pubmed/35755058 http://dx.doi.org/10.3389/fmed.2022.847407 Text en Copyright © 2022 Kong, Wang, Fang, Yang and Tong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Kong, Xiang-Qi
Wang, Yong-Jing
Fang, Zan-Xi
Yang, Tian-Ci
Tong, Man-Li
False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators
title False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators
title_full False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators
title_fullStr False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators
title_full_unstemmed False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators
title_short False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators
title_sort false-positive results of sars-cov-2 rt-pcr in oropharyngeal swabs from vaccinators
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226675/
https://www.ncbi.nlm.nih.gov/pubmed/35755058
http://dx.doi.org/10.3389/fmed.2022.847407
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