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National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel

In this report, we describe a national-scale monitoring of the SARS-CoV-2 (SC-2) variant dynamics in Israel, using multiple-time sampling of 13 wastewater treatment plants. We used a combination of inclusive and selective quantitative PCR assays that specifically identify variants A19/A20 or B.1.1.7...

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Autores principales: Bar-Or, Itay, Indenbaum, Victoria, Weil, Merav, Elul, Michal, Levi, Nofar, Aguvaev, Irina, Cohen, Zvi, Levy, Virginia, Azar, Roberto, Mannasse, Batya, Shirazi, Rachel, Bucris, Efrat, Mor, Orna, Sela Brown, Alin, Sofer, Danit, Zuckerman, Neta S., Mendelson, Ella, Erster, Oran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227326/
https://www.ncbi.nlm.nih.gov/pubmed/35746700
http://dx.doi.org/10.3390/v14061229
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author Bar-Or, Itay
Indenbaum, Victoria
Weil, Merav
Elul, Michal
Levi, Nofar
Aguvaev, Irina
Cohen, Zvi
Levy, Virginia
Azar, Roberto
Mannasse, Batya
Shirazi, Rachel
Bucris, Efrat
Mor, Orna
Sela Brown, Alin
Sofer, Danit
Zuckerman, Neta S.
Mendelson, Ella
Erster, Oran
author_facet Bar-Or, Itay
Indenbaum, Victoria
Weil, Merav
Elul, Michal
Levi, Nofar
Aguvaev, Irina
Cohen, Zvi
Levy, Virginia
Azar, Roberto
Mannasse, Batya
Shirazi, Rachel
Bucris, Efrat
Mor, Orna
Sela Brown, Alin
Sofer, Danit
Zuckerman, Neta S.
Mendelson, Ella
Erster, Oran
author_sort Bar-Or, Itay
collection PubMed
description In this report, we describe a national-scale monitoring of the SARS-CoV-2 (SC-2) variant dynamics in Israel, using multiple-time sampling of 13 wastewater treatment plants. We used a combination of inclusive and selective quantitative PCR assays that specifically identify variants A19/A20 or B.1.1.7 and tested each sample for the presence and relative viral RNA load of each variant. We show that between December 2020 and March 2021, a complete shift in the SC-2 variant circulation was observed, where the B.1.1.7 replaced the A19 in all examined test points. We further show that the normalized viral load (NVL) values and the average new cases per week reached a peak in January 2021 and then decreased gradually in almost all test points, in parallel with the progression of the national vaccination campaign, during February–March 2021. This study demonstrates the importance of monitoring SC-2 variant by using a combination of inclusive and selective PCR tests on a national scale through wastewater sampling, which is far more amendable for high-throughput monitoring compared with sequencing. This approach may be useful for real-time dynamics surveillance of current and future variants, such as the Omicron (BA.1, BA.2) and other variants.
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spelling pubmed-92273262022-06-25 National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel Bar-Or, Itay Indenbaum, Victoria Weil, Merav Elul, Michal Levi, Nofar Aguvaev, Irina Cohen, Zvi Levy, Virginia Azar, Roberto Mannasse, Batya Shirazi, Rachel Bucris, Efrat Mor, Orna Sela Brown, Alin Sofer, Danit Zuckerman, Neta S. Mendelson, Ella Erster, Oran Viruses Article In this report, we describe a national-scale monitoring of the SARS-CoV-2 (SC-2) variant dynamics in Israel, using multiple-time sampling of 13 wastewater treatment plants. We used a combination of inclusive and selective quantitative PCR assays that specifically identify variants A19/A20 or B.1.1.7 and tested each sample for the presence and relative viral RNA load of each variant. We show that between December 2020 and March 2021, a complete shift in the SC-2 variant circulation was observed, where the B.1.1.7 replaced the A19 in all examined test points. We further show that the normalized viral load (NVL) values and the average new cases per week reached a peak in January 2021 and then decreased gradually in almost all test points, in parallel with the progression of the national vaccination campaign, during February–March 2021. This study demonstrates the importance of monitoring SC-2 variant by using a combination of inclusive and selective PCR tests on a national scale through wastewater sampling, which is far more amendable for high-throughput monitoring compared with sequencing. This approach may be useful for real-time dynamics surveillance of current and future variants, such as the Omicron (BA.1, BA.2) and other variants. MDPI 2022-06-06 /pmc/articles/PMC9227326/ /pubmed/35746700 http://dx.doi.org/10.3390/v14061229 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bar-Or, Itay
Indenbaum, Victoria
Weil, Merav
Elul, Michal
Levi, Nofar
Aguvaev, Irina
Cohen, Zvi
Levy, Virginia
Azar, Roberto
Mannasse, Batya
Shirazi, Rachel
Bucris, Efrat
Mor, Orna
Sela Brown, Alin
Sofer, Danit
Zuckerman, Neta S.
Mendelson, Ella
Erster, Oran
National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel
title National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel
title_full National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel
title_fullStr National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel
title_full_unstemmed National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel
title_short National Scale Real-Time Surveillance of SARS-CoV-2 Variants Dynamics by Wastewater Monitoring in Israel
title_sort national scale real-time surveillance of sars-cov-2 variants dynamics by wastewater monitoring in israel
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227326/
https://www.ncbi.nlm.nih.gov/pubmed/35746700
http://dx.doi.org/10.3390/v14061229
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