Cargando…

Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production

Vertical transmission is a consistently discussed pathway of porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) transmission in pigs. To evaluate the presence of PCV2 and PCV3 in piglets, we collected tissue samples from 185 piglets that were crushed within the first week of life...

Descripción completa

Detalles Bibliográficos
Autores principales: Eddicks, Matthias, Maurer, Roland, Deffner, Pauline, Eddicks, Lina, Sipos, Wolfgang, Reese, Sven, Cvjetković, Vojislav, Krejci, Roman, Opriessnig, Tanja, Ritzmann, Mathias, Fux, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227362/
https://www.ncbi.nlm.nih.gov/pubmed/35745525
http://dx.doi.org/10.3390/pathogens11060671
_version_ 1784734156143460352
author Eddicks, Matthias
Maurer, Roland
Deffner, Pauline
Eddicks, Lina
Sipos, Wolfgang
Reese, Sven
Cvjetković, Vojislav
Krejci, Roman
Opriessnig, Tanja
Ritzmann, Mathias
Fux, Robert
author_facet Eddicks, Matthias
Maurer, Roland
Deffner, Pauline
Eddicks, Lina
Sipos, Wolfgang
Reese, Sven
Cvjetković, Vojislav
Krejci, Roman
Opriessnig, Tanja
Ritzmann, Mathias
Fux, Robert
author_sort Eddicks, Matthias
collection PubMed
description Vertical transmission is a consistently discussed pathway of porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) transmission in pigs. To evaluate the presence of PCV2 and PCV3 in piglets, we collected tissue samples from 185 piglets that were crushed within the first week of life from 16 farms located in Germany and Austria. Pooled samples consisting of thymus, inguinal lymph node, myocardium, lung and spleen were examined for PCV2 and PCV3 by qPCR. Furthermore, oral fluid samples (OFS) from grow–finish pigs were collected and examined the same way. In piglets, PCV2 was highly prevalent (litters: 69.4%; piglets: 61.6%), whereas PCV3 prevalence was low (litters: 13.4%; piglets: 13.0%). In total, 72.6% and 67.2% of all collected OFS were PCV2 or PCV3 positive, respectively. Sow vaccination against PCV2 was identified as a protective factor concerning PCV2 in piglets (OR: 0.279; CI: 0.134–0.578; p < 0.001), whereas the porcine reproductive and respiratory syndrome virus (PRRSV) vaccination of sows was identified as a protective factor concerning PCV3 in piglets (OR: 0.252 CI: 0.104–0.610; p = 0.002). Our results show that PCV2, but not PCV3, is ubiquitous in suckling piglets and that early PCV3 infections might be modulated by PRRSV–PCV3 interaction. However, the ubiquitous nature of both viruses in older pigs could be confirmed.
format Online
Article
Text
id pubmed-9227362
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92273622022-06-25 Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production Eddicks, Matthias Maurer, Roland Deffner, Pauline Eddicks, Lina Sipos, Wolfgang Reese, Sven Cvjetković, Vojislav Krejci, Roman Opriessnig, Tanja Ritzmann, Mathias Fux, Robert Pathogens Article Vertical transmission is a consistently discussed pathway of porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) transmission in pigs. To evaluate the presence of PCV2 and PCV3 in piglets, we collected tissue samples from 185 piglets that were crushed within the first week of life from 16 farms located in Germany and Austria. Pooled samples consisting of thymus, inguinal lymph node, myocardium, lung and spleen were examined for PCV2 and PCV3 by qPCR. Furthermore, oral fluid samples (OFS) from grow–finish pigs were collected and examined the same way. In piglets, PCV2 was highly prevalent (litters: 69.4%; piglets: 61.6%), whereas PCV3 prevalence was low (litters: 13.4%; piglets: 13.0%). In total, 72.6% and 67.2% of all collected OFS were PCV2 or PCV3 positive, respectively. Sow vaccination against PCV2 was identified as a protective factor concerning PCV2 in piglets (OR: 0.279; CI: 0.134–0.578; p < 0.001), whereas the porcine reproductive and respiratory syndrome virus (PRRSV) vaccination of sows was identified as a protective factor concerning PCV3 in piglets (OR: 0.252 CI: 0.104–0.610; p = 0.002). Our results show that PCV2, but not PCV3, is ubiquitous in suckling piglets and that early PCV3 infections might be modulated by PRRSV–PCV3 interaction. However, the ubiquitous nature of both viruses in older pigs could be confirmed. MDPI 2022-06-10 /pmc/articles/PMC9227362/ /pubmed/35745525 http://dx.doi.org/10.3390/pathogens11060671 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eddicks, Matthias
Maurer, Roland
Deffner, Pauline
Eddicks, Lina
Sipos, Wolfgang
Reese, Sven
Cvjetković, Vojislav
Krejci, Roman
Opriessnig, Tanja
Ritzmann, Mathias
Fux, Robert
Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production
title Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production
title_full Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production
title_fullStr Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production
title_full_unstemmed Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production
title_short Cross-Sectional Study on the Prevalence of PCV Types 2 and 3 DNA in Suckling Piglets Compared to Grow–Finish Pigs in Downstream Production
title_sort cross-sectional study on the prevalence of pcv types 2 and 3 dna in suckling piglets compared to grow–finish pigs in downstream production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227362/
https://www.ncbi.nlm.nih.gov/pubmed/35745525
http://dx.doi.org/10.3390/pathogens11060671
work_keys_str_mv AT eddicksmatthias crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT maurerroland crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT deffnerpauline crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT eddickslina crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT siposwolfgang crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT reesesven crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT cvjetkovicvojislav crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT krejciroman crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT opriessnigtanja crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT ritzmannmathias crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction
AT fuxrobert crosssectionalstudyontheprevalenceofpcvtypes2and3dnainsucklingpigletscomparedtogrowfinishpigsindownstreamproduction